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Effects of traditional Chinese medicine “Yimu Shenghuatang” on cytochrome P450 in cow inflammatory endometrial cells

  • Research Article
  • Published:
Frontiers of Agriculture in China

Abstract

To investigate the metabolism mechanism of traditional Chinese medicine “Yimu Shenghuatang” on dairy cow endometrial cells, the primary cultured endometrial cells in dairy cows were isolated by a collagenase digestion method, and an inflammatory model of the endometrial cells by lipopolysaccharide (LPS) was made. The cells were treated with gradient concentrations of LPS, the cultured supernatants were collected in different periods, and the contents of TNF-α, IL-1β were determined by Enzyme-linked immunosorbent assay (ELISA). The results showed that 100 ng·mL−1 LPS was the optimal concentration to induce the inflammation of cultured endometrial cells. The inflammatory endometrial cells were treated with traditional Chinese medicine “Yimu Shenghuatang” and its main components during the period of 24–72 h, and the expression of cytochrome P450 in cow endometrial cells was determined by Western blot. Our result lays a foundation for treating cow endometritis by using traditional Chinese medicines.

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References

  • Abdel-Salam B K, Ebaid H (2008). Upregulation of major histocompatibility complex class II, CD83, CD64, and CD14 on polymorphonuclear neutrophils stimulated with interleukin-15. J Microbiol Immunol Infect, 41(6): 462–468

    PubMed  CAS  Google Scholar 

  • Azawi O I (2008). Postpartum uterine infection in cattle. Anim Reprod Sci, 105(3–4): 187–208

    Article  PubMed  CAS  Google Scholar 

  • Banu S K, Lee J, Speights V O Jr, Starzinski-Powitz A, Arosh J A (2008). Cyclooxygenase-2 regulates survival, migration, and invasion of human endometriotic cells through multiple mechanisms. Endocrinology, 149(3): 1180–1189

    Article  PubMed  CAS  Google Scholar 

  • Hu Y, Ouyang W Q, Sun J C, Wang X F, Zhao X D (2007). Preparation of compound furacilin nanoemulsion and its clinic therapeutic effect on bovine endometritis. Journal of Northwest A&F University, 37(9): 18–22 (in Chinese)

    Google Scholar 

  • Ishikawa T, Harada T, Kubota T, Aso T (2007). Testosterone inhibits matrix metalloproteinase-1 production in human endometrial stromal cells in vitro. Reproduction, 133(6): 1233–1239

    Article  PubMed  CAS  Google Scholar 

  • Jia X L, Qi C M (2005). Advance of dairy cow endometritis. Chin J Vet Med, 41(2): 34–36 (in Chinese)

    Google Scholar 

  • LeBlanc S J (2008). Postpartum uterine disease and dairy herd reproductive performance: a review. Vet J, 176(1): 102–114

    Article  PubMed  Google Scholar 

  • LeBlanc S J, Duffield T F, Leslie K E, Bateman K G, Keefe G P, Walton J S, Johnson W H (2002). The effect of treatment of clinical endometritis on reproductive performance in dairy cows. J Dairy Sci, 85(9): 2237–2249

    Article  PubMed  CAS  Google Scholar 

  • Leung S T, Cheng Z, Sheldrick E L, Derecka K, Derecka K, Flint A P, Wathes D C (2001). The effects of lipopolysaccharide and interleukins-1alpha, -2 and -6 on oxytocin receptor expression and prostaglandin production in bovine endometrium. J Endocrinol, 168(3): 497–508

    Article  PubMed  CAS  Google Scholar 

  • Li D J, Guo D Z, Pei X Y (2010). Oxidative damage and changes of antioxidant defense capability in postpartum endometritis cows. China Animal Husbandry & Veterinary Medicine, 37(4): 174–177 (in Chinese)

    CAS  Google Scholar 

Download references

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Correspondence to Yuzhong Ma.

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Du, J., Qin, J., Chu, J. et al. Effects of traditional Chinese medicine “Yimu Shenghuatang” on cytochrome P450 in cow inflammatory endometrial cells. Front. Agric. China 5, 102–105 (2011). https://doi.org/10.1007/s11703-010-1046-6

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  • DOI: https://doi.org/10.1007/s11703-010-1046-6

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