Abstract
An efficient extraction method by ultrasound-assisted extraction (UAE) was developed for the extraction of polychlorinated biphenyls (PCB 28, 52, 101, 118, 130, 153, 180) from various shellfish. Analytes were determined by gas chromatography-mass spectrometry in selected ion monitoring (SIM) mode. All the samples were extracted by n-hexane and decontaminated by concentrated sulfuric acid. The resulting samples were determined by quantitative detection by comparing with external standards. The average recoveries of seven kinds of PCBs ranged from 80.92% to 93.89%, and the lowest detectable limit was 0.08-0.03 μg·kg−1. The average concentrations of total PCBs were 14.12–30.61 μg·kg−1 in the samples. This method was highly effective in reducing the cost and time for the pretreatment of samples. This method may be applied for the screening and monitoring of shellfish for organic pollutants in coastal waters.
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References
Alfonso D M, Tiziana G, Danilo A B (1997). Single-step separation of organochlorine pesticide residues from fatty materials by combined use of solid-matrix partition and C18 cartridges. Journal of Chromatography A, 765(1): 61–68
Berzas J J B, Martín-Doimeadiós R C R, Guzmán F J, Rodríguez N (2007). Development and validation of an analytical methodology for the determination of p,p-DDT, p,p-DDE and p,p-DDD in fish oil pills. Microchemical Journal, 86(2): 183–188
Bommanna G L, Kurunthachalam K (1994). Global organochlorine contamination trends: An overview. Ambio, 23(3): 187–191
FAO, IOC, IAEA (1993). Guidelines for monitoring chemical contaminants in the sea using marine organisms. Reference Methods for Marine Pollution Studies, No. 6, UNDP
Fontana A R, Silva M F, Martínez L D, Wuilloud R G (2009). Determination of polybrominated diphenyl ethers in water and soil samples by cloud point extraction-ultrasound-assisted back-extraction-gas chromatography-mass spectrometry. Journal of Chromatography A, 1216(20): 4339–4346
Gabriela R, Carla T, Inés A, Betsabet S (2008). Determination of polychlorinated biphenyls in biosolids using continuous ultrasound-assisted pressurized solvent extraction and gas chromatography-mass spectrometry. Journal of Chromatography A, 1193(1–2): 32–36
GB9674 - 88. Tolerance limit of polychlorobiphenyls (PCBs) in marine foods, China (S)
GB/T5009.190 - 2006. Determination of indicator polychlorinated biphenyls in foods, China (S)
Hutzinger O, Safe S, Zitko V (1974). The Chemistry of PCBs. Cleveland: CRC Press, 269
José L T, Consuelo S B, Beatriz A, Ana I (2010). Application of ultrasound-assisted extraction to the determination of contaminants in food and soil samples. Journal of Chromatography A, 1217(16): 2415–2440
Knorr D, Zenker M, Heinz V (2004). Applications and potential of ultrasonics in food processing. Trends in Food Science & Technology, 15(5): 261–266
Marc C, Martine B, Marie-Jeanne T, Anne-Marie C, Paul T, André C (2006). Evaluation of the pollution by organochlorinated compounds (polychlorobiphenyls and pesticides) and metals (Cd, Cr, Cu and Pb) in the water and in the zebra mussel (Dreissena polymorpha pallas) of the river Seine. Water, Air, & Soil Pollution, 88(3–4): 371–381
Nevado J J B, Martín-Doimeadiós R C, Guzmán Bernardo F J, Fariñas N R (2010). Multiresidue determination of organochlorines in fish oil by GC-MS: a new strategy in the sample preparation. Talanta, 81(3): 887–893
Orlinskii D, Priputina I, Popova A, Shalanda A, Tsongas T, Hinman G, Butcher W (2001). Influence of environmental contamination with PCBs on human health. Environmental Geochemistry and Health, 23(4): 313–328
Ozcan S, Tor A, Aydin M E (2009). Determination of selected polychlorinated biphenyls in water samples by ultrasound-assisted emulsification-microextraction and gas chromatography-mass-selective detection. Analytica Chimica Acta, 647(2): 182–188
Patel K, Fussell R J, Hetmanski M, Goodall D M, Keely B J (2005). Evaluation of gas chromatography-tandem quadrupole mass spectrometry for the determination of organochlorine pesticides in fats and oils. Journal of Chromatography A, 1068(2): 289–296
Patist A, Bates D (2008). Ultrasonic innovations in the food industry: From the laboratory to commercial production. Innovative Food Science & Emerging Technologies, 9(2): 147–154
SC/T-3111-2006. Quick frozen scallop, China (S)
Stephen A, Gian M C, David S (1997). GC/MS procedures for the determination of PCBs in environmental matrices. Chemosphere, 35(5): 905–917
Wu Y, Zhang J, Zhou Q (1999). Persistent orgnochlorine residues in sediments from Chinese river/estuary systems. Environmental Pollution, 105(1): 143–150
Yang N, Matsuda M, Kawano M (2006). PCBs and OCPs in edible fish and shellfish from China. Chemosphere, 63: 1342–1352
Zhou J L, Hong H, Zhang Z, Maskaoui K, Chen W (2000). Multi-phase distribution of organic micropollutants in Xiamen Harbour. Water Research, 34(7): 2132–2150
Zhou J L, Maskaoui K, Qiu Y W, Hong H S, Wang Z D (2001). Polychlorinated biphenyl congeners and organochlorine insecticides in the water column and sediments of Daya Bay, China. Environmental Pollution, 113(3): 373–384
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Zhou, Q., Sang, Y., Wang, L. et al. Determination of polychlorinated biphenyls by GC/MS with ultrasound-assisted extraction from shellfish. Front. Agric. China 4, 489–493 (2010). https://doi.org/10.1007/s11703-010-1032-8
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DOI: https://doi.org/10.1007/s11703-010-1032-8