Abstract
Diethylstilbestrol-hemi-succinate (DES-HS) and diethylstilbestrol-monocarboxylic-propyl-ether (DESMCPE) were synthesized in different ways. The two kinds of haptens were conjugated to bovine serum albumin (BSA) and ovalbumin (OVA) to form the immuno-antigens and the coating-antigen, respectively. The antibody with high titer was obtained after immunising rabbits. The results showed that the antibody had a high affinity to diethylstilbestrol tested by enzyme-linked immunosorbent assay (ELISA) and Western blot. This study lays a basis for further research on the immune test kit for the DES remnant.
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Fan, Q., Wang, X., Zhou, Q. et al. Synthesis of artificial antigens of diethylstilbestrol and preparation of its antibody. Front. Agric. China 4, 188–194 (2010). https://doi.org/10.1007/s11703-010-0097-8
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DOI: https://doi.org/10.1007/s11703-010-0097-8