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Biological characteristics of Bacillus thuringiensis strain Bt11 and identification of its cry-type genes

  • Research Article
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Frontiers of Agriculture in China

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Abstract

A novel strain of Bacillus thuringiensis Bt11, isolated from soil samples in China, was classified and characterized in terms of its crystal proteins, cry genes content. The Bt11 strain showed high toxicity against Spodoptera exigua and Helicoverpa armigera neonates. Bt11 strain shares morphological and biochemical characteristics with the previously described Bacillus thuringiensis subsp. kurstaki. SDS-polyacrylamide gel electrophoresis revealed that crystals were composed of several polypeptides ranging from 20 to 130 kDa, of which the 35, 80, and 130 kDa proteins were the major components. PCR-RFLP with total DNA from strain Bt11 and specific primers for cry1, cry2, cry3, cry4/10, cry7, cry8, cry9, and cry11 genes revealed that cry1Aa, cry1Ab, cry1Ia, and cry9Ea genes were present.

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Authors and Affiliations

  1. College of Plant Protection, Agricultural University of Hebei, Biological Control Center of Plant Diseases and Plant Pests of Hebei Province, Baoding, 071001, China

    Tinghui Liu & Wei Guo

  2. College of Life Sciences, Agricultural University of Hebei, Baoding, 071001, China

    Wei Guo, Weiming Sun & Yongxiang Sun

Authors
  1. Tinghui Liu
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  2. Wei Guo
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  3. Weiming Sun
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  4. Yongxiang Sun
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Correspondence to Wei Guo.

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Liu, T., Guo, W., Sun, W. et al. Biological characteristics of Bacillus thuringiensis strain Bt11 and identification of its cry-type genes. Front. Agric. China 3, 159–163 (2009). https://doi.org/10.1007/s11703-009-0040-z

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  • DOI: https://doi.org/10.1007/s11703-009-0040-z

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