Frontiers of Agriculture in China

, Volume 2, Issue 3, pp 314–316

Multicolor FISH analysis of rDNA and telomere on spinach

  • Tianying Lan
  • Bo Liu
  • Fengping Dong
  • Ruiyang Chen
  • Xiulan Li
  • Chengbin Chen
Research Article

DOI: 10.1007/s11703-008-0059-6

Cite this article as:
Lan, T., Liu, B., Dong, F. et al. Front. Agric. China (2008) 2: 314. doi:10.1007/s11703-008-0059-6
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Abstract

In this study, multicolor fluorescence in situ hybridization (FISH) analysis on metaphase chromosomes of spinach with biotin-labeled 25S rDNA, DIG-labeled telomere sequences and biotin-labeled and DIG-labeled 5S rDNA was performed. There were six 25S rDNA loci located on the satellites of the third, the fifth and the sixth chromosomes, and four 5S rDNA loci located on the long arms of the third and the fifth chromosomes. The telomere loci were located on the end of the sixth chromosome and also on both the end and centromeric regions of other chromosomes. This study is an important complement to both traditional karyotype analysis and FISH karyotype analysis in spinach.

Keywords

spinach multicolor FISH rDNA telomere 

Copyright information

© Higher Education Press and Springer-Verlag GmbH 2008

Authors and Affiliations

  • Tianying Lan
    • 1
  • Bo Liu
    • 1
  • Fengping Dong
    • 1
  • Ruiyang Chen
    • 1
  • Xiulan Li
    • 1
  • Chengbin Chen
    • 1
  1. 1.Molecular Cytogenetics Research Laboratory, College of Life SciencesNankai UniversityTianjinChina

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