Abstract
Starch granule proteins (SGPs) are minor components bound with starch granule, whose variation could impact starch properties. This study investigated, for the first time, the variation of SGPs in the cultivated naked barley from Qinghai-Tibet Plateau in China. The relationship between SGPs and starch content was preliminarily dealt with. Ten major SGPs and 16 types of patterns were present in the 66 cultivated naked varieties, indicating that the SGPs in cultivated naked barley from Qinghai-Tibet Plateau in China are polymorphic. The SGPs of naked barley in Tibet and Sichuan were greatly different and the SGP patterns were specific to sampling regions. Significance test analysis demonstrated that the SGPs described in this study, except for SGP1, could be related with the variation of starch content in the different naked barleys.
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References
Bernardo A, Luque A, Cuadrado A, Negro A, Jouve N, Soler C (1997). The assessment of genetic variation in Spanish primitive cultivars of barley, Hordeum vulgare L., by a combination of isozyme and hordeins. Genet Res Crop Evol, 44(3): 217–226
Burton R A, Jenner H, Carrangis L, Fahy B, Finche G B, Hylton C, Laurie D A, Parker M, Waite D, van Wegen S, Verhoeven T, Denyer K (2002). Starch granule initiation and growth are altered in barley mutants that lack isoamylase activity. Plant Journal, 31(1): 97–112
Denyer K, Hylton C M, Jenner C F, Smith A M (1995). Identification of multiple isoforms of soluble and granule-bound starch synthase in developing wheat endosperm. Planta, 196: 256–265
Edwards A, Fulton D C, Hylton C M, Jobling S A, Gidley M, Rössner U, Martin C, Smith A M (1999). A combined reduction in activity of starch synthases II and III of potato has novel effects on the starch of tubers. Plant J, 17(11): 251–261
Ishikawa N, Ishihara J, Itoh M (1994). Artificial induction and characterization of amylose-free mutants of barley. Barley Genet Newsletter, 24: 49–53
Johnson P E, Patron N J, Bottrill A R, Dinges J R, Fahy, B F, Parker M L, Waite D N, Denyer K A (2003). Low-starch barley mutant, Risø 16, lacking the cytosolic small subunit of ADP-glucose pyrophosphorylase, reveals the importance of the cytosolic isoform and the identity of the plastidial small subunit. Plant Physiology, 131(2): 684–696
Morell M K, Kosar-Hashemi B, Cmiel M, Samuel M S, Chandler P, Rahman S, Buleon A, Batey I L, Li Z (2003). Barley sex6 mutants lack starch synthase Iia activity and contain a starch with novel properties. Plant Journal, 34(13): 173–185
Morrison W R, Laignelet B (1983). An improved colorimetric procedure for determining apparent and total amylose in cereal and other starches. Journal of Cereal Science, 2: 257–271
Mouille G, Maddelein M L, Libessart N, Talaga P, Decq A, Delrue B, Ball S (1996). Preamylopectin processing: a mandatory step for starch biosynthesis in plants. Plant Cell, 8(8): 1353–1366
Pan Z F, Deng G B, Wang T, Yu M Q (2000). An improved 1DSDS-PAGE method for identification of wheat waxy protein. Chin J Appl Environ Biol, 6(5): 487–489 (in Chinese)
Peng M S, Gao M, Båga M (2000). Starch-branching enzymes preferentially associated with A-type starch granules in wheat endosperm. Plant Physiol, 124(1): 265–272 (in Chinese)
Rahman S, Hashemi B K, Samuel M S, Hill A, Abbott D C, Skerritt J H, Preiss J, Appels R, Morell M K (1995). The major proteins of wheat endosperm starch granules. Aust J Plant Physiol, 22: 793–803
Rohlf F J (1998). NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System, version 2.0, Exeter Software, New York
Schulman A H, Ahokas H (1990). A novel shrunken endosperm mutant of barley. Physiol Plant, 78(4): 583–589
Shure M, Wessler S, Fedoroff N (1983). Molecular identification and isolation of waxy locus in maize. Cell, 35: 225–233
Sulaiman B D, Morrison W R (1990). Proteins associated with the surface of wheat starch granules purified by centrifuging through cesium chloride. J Cereal Sci, 12: 53–61
Tyynela J, Schulman A H (1993). An analysis of soluble starch synthase isozymes from the developing grains of normal and shx cv. Bomi barley (Hordeum vulgare). Physiol Plant, 89(4): 835–841
Tyynela J, Stitt M, Lönneborg A, Smeekens S, Schulman A H (1995). Metabolism of starch synthases in developing grains of the shx shrunken mutant of barley (Hordeum vulgare). Physiol Plant, 93(1): 77–84
Yamamori M, Endo T R (1996). Variation of starch granule proteins and chromosome mapping of their coding genes in common wheat. Theor Appl Genet, 93(2): 275–281
Yamamori M, Fujita S, Hayakawa K, Matsuki J, Yasui T (2000). Genetic elimination of a starch granule protein, SGP-1, of wheat generates an altered starch with apparent high amylose. Theor Appl Genet, 101(1): 21–29
Yeh F C, Yang R C, Tim B (1999). POPGENE: Microsoft Window-based Freeware for Population Genetic Analysis. Version 1.31
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Translated from Hereditas, 2007, 29(5): 599–606 [译自: 遗传]
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Pan, Z., Zou, Y., Zhao, T. et al. Starch granule protein (SGP) polymorphism in cultivated naked barley from Qinghai-Tibet Plateau in China and relationship between SGPs and starch/amylose content. Front. Agric. China 2, 10–15 (2008). https://doi.org/10.1007/s11703-008-0018-2
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DOI: https://doi.org/10.1007/s11703-008-0018-2