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Integration of C4-specific PPDK gene of maize to C3 rice and its characteristics in relation to photosynthesis

Abstract

Pyruvate orthophosphate dikinase (PPDK) is a key enzyme in photosynthesis in some plants that exploit the C4 photosynthetic pathway for the fixation of CO2. The C4-specific PPDK encoding pyruvate orthophosphate dikinase was introduced into C3 plant, a rice (Oryza sativa L. cv. indica IR64) mediated by biolistic and Agrobacterium transformation. The C4-PPDK gene of maize was integrated to indica IR64 with polymerase chain reaction (PCR)-Southern blotting. The total nitrogen of flag leaves of transgenic IR64 was analyzed with Kjeldahl method for quantitative determination of nitrogen, indicating that the total nitrogen of flag leaves of most transgenic IR64 was higher than that of non-transgenic control IR64 formants in the greenhouse. The maximum value of total nitrogen of flag leaves was 3.61% among transgenic IR64 plants, 1.07% higher than that of non-transgenic control IR64 formants. The total nitrogen of flag leaves of transgenic IR64 was increased by 42.1%. The factors for yield of transgenic IR64 plants were analyzed, indicating there was a greater difference in yield-forming factors among transgenic IR64 plants in the greenhouse, i.e. dried plant weight, harvested index and so on. Thus, it could help rice breeders select different materials for breeding.

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References

  1. Bowes G, Ogren W L, Hageman R H (1971). Phosphoglycolate production catalyzed by ribulose diphosphate carboxylase. Biochem Biophys Res Commun, 45: 716–722

    PubMed  Article  CAS  Google Scholar 

  2. Brown N J, Parsley K, Hibberd J M (2005). The future of C4 research-maize, Flaveria or Cleome? Trends in Plant Science, 10(5): 215–221

    PubMed  Article  CAS  Google Scholar 

  3. Datta S K, Datta K, Soltanifar N, Donn G, Potrykus I (1992). Herbicide-resistant indica rice piants from IRRI breeding line IR 72 after PEG-mediated transformation of protoplasts. Plant Mol Biol, 20: 619–629

    PubMed  Article  CAS  Google Scholar 

  4. Datta S K, Torrizo L B, Tu J M, Oliva N P, Datta K (1997). Agrobacterium-mediated transformation, production and molecular evaluation of transgenic rice. In: International Rice Research Institute ed. IRRI Discussion Paper Series N0. 21. Manila: IRRI, 20–22

    Google Scholar 

  5. Deane-Drummond C E, Clarkson D T (1979). Effect of shoot removal and malate on the activity of nitrate reductase assayed in vivo in barley roots (Hordeum vulgare cv. Midas). Plant Physiol, 64: 660–662

    PubMed  CAS  Article  Google Scholar 

  6. Dellaporta S L, Wood J, Hicks J B (1983). A plant DNA minipreparation: Version II. Plant Mol Biol Rep, 1(4): 19–21

    CAS  Article  Google Scholar 

  7. Dunwell J M (2000). Transgenic approaches to crop improvement. Journal of Experimental Botany, 51: 487–496

    PubMed  Article  CAS  Google Scholar 

  8. Edwards G E, Ku M S B, Hatch M D (1982). Photosynthesis in Panicum milioides, a species with reduced photorespiration. Plant Cell Physiol, 23: 1,185–1,195

    CAS  Google Scholar 

  9. Edwards G E, Nakamoto H, Burnell J N, Hatch M D (1985). Pyruvate, Pi dikinase and NADP-malate dehydrogenase in C4 photosynthesis: Properties and mechanism of light/dark regulation. Annu Rev Plant Physiol, 36: 255–286

    Article  CAS  Google Scholar 

  10. Fukayama H, Tsuchida H, Agarie S, Nomura M, Onodera H, Ono K, Lee B-H, Hirose S, Toki S, Ku M S B, Makino A, Matsuoka M, Miyao M (2001). Significant accumulation of C4-specific pyruvate, orthophosphate dikinase in a C3 plant, rice. Plant Physiol, 127: 1136–1146

    PubMed  Article  CAS  Google Scholar 

  11. Hatch M D, Slack C R (1986). A new enzyme for the interconversion of pyruvate and phosphoenolpyruvate and its role in the C4 dicarboxylic acid pathway of photosynthesis. Biochem J, 106: 141–146

    Google Scholar 

  12. Hatch, M D (1987). C4 photosynthesis: A unique blend of modified biochemistry, anatomy and ultrastructure. Biochim Biophys Acta, 895: 81–106

    CAS  Google Scholar 

  13. Hatch, M D (1978). Regulation of enzymes in C4 photosynthesis. Curr Top Cell Reg, 14: 1–27

    CAS  Google Scholar 

  14. Kluge M, Osmond C B (1971). Pyruvate Pi dikinase in crassulaceous acid metabolism. Naturwissenschaften, 58: 414–415

    Article  CAS  Google Scholar 

  15. Matsuoka M (1995). The gene for pyruvate, orthophosphate dikinase in C4 plants: Structure, regulation and evolution: Amini review. Plant Cell Physiol, 36: 937–943

    PubMed  CAS  Google Scholar 

  16. McCormac D, Boinski Joseph J, Ramsperger Vincent C, Berry James O (1997). C4 gene expression in photosynthetic and nonphotosynthetic leaf regions of Amaranfhus trkolor. Plant Physiol, 114: 801–815

    PubMed  CAS  Google Scholar 

  17. Meyer A O, Kelly G J, Latzko E (1982). Pyruvate orthophosphate-dikinase from the immature grain of cereal grasses. Plant Physiol, 69: 7–10

    PubMed  CAS  Google Scholar 

  18. Miyao-Tokutomi M, Fukayama H (2005). Overproduction of C4 enzymes in transgenic rice: An approach for improved photosynthesis and crop yield. In: Toriyama K, Heong K L, Hardy B, eds. Proceedings of World Rice Research Conference “Rice is life: Scientific perspectives for the 21st century”. Los Baños (Philippines): International Rice Research Institute, and Tsukuba (Japan): Japan International Research Center for Agricultural Sciences, 87–90

    Google Scholar 

  19. Nelson T, Langdale J A (1992). Developmental genetics of C4 photosynthesis. Annu Rev Plant Physiol Plant Mol Biol, 43: 25–47

    Article  CAS  Google Scholar 

  20. Oaks A (1994). Efficiency of nitrogen utilization in C3 and C4 cereals. Plant Physiol, 106: 407–414

    PubMed  CAS  Google Scholar 

  21. Oaks Ann, Hirel B (1985). Nitrogen metabolism in roots. Ann Rev Plant Physiol, 36: 345–365

    CAS  Google Scholar 

  22. Sugiyama T (1998). Nitrogen-responsive expression of C4 photosynthesis genes in maize. In: Satoh K, Murata N, eds. Stress Responses of Photosynthetic Organisms. Tokyo: Elsevier, 167–180

    Google Scholar 

  23. Tobin E M, Silverthome J (1985). Light regulation of gene expression in higher plants. Annu Rev Plant Physiol, 36: 569–593

    Article  CAS  Google Scholar 

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Correspondence to Wang Guoying or Xie Huaan.

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Translated from Molecular Plant Breeding, 2006, 4(6): 797–804 [译自: 分子植物育种]

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Zhang, J., Datta, S.K., Wang, G. et al. Integration of C4-specific PPDK gene of maize to C3 rice and its characteristics in relation to photosynthesis. Front. Agric. China 1, 243–249 (2007). https://doi.org/10.1007/s11703-007-0042-7

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Keywords

  • characteristics
  • integration
  • maize (Zea mays)
  • photosynthesis
  • pyruvate orthophosphate dikinase (PPDK) gene
  • rice (Oryza sativa L.)