Abstract
In this study, polyphenols from sweet potato leaves were purified by macroporous resin, and antioxidant activity was evaluated by free radical scavenging. AB-8, D-101, HPD-100 and DM301 four resins were selected for purification. The antioxidant activity was evaluated by 2,4-dinitrophenylhydrazine (DPPH) and 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulfonate) (ABTS) methods. The results showed that the DM-301 macroporous adsorption resin was most suitable by static adsorption–desorption method, and the optimal purification conditions were determined by single factor experiment as follows: the concentration of sample solution 6.0 mg/mL, the flow rate of sample solution 60 mL/min, the volume fraction of ethanol desorption 60%, and the flow rate 40 mL/h. The scavenging rates of DPPH and ABTS free radicals by purified polyphenols from sweet potato leaves were 74.04% and 98.20%, respectively. The research results provide reference for further development and utilization of polyphenols from sweet potato leaves.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ahmadi S, Lecompagnon J, Hirsch PD, Burgholzer P, Jung P, Caire G, Ziegler M (2020) Laser excited super resolution thermal imaging for nondestructive inspection of internal defects. Sci Rep 10(1):22357. https://doi.org/10.1038/s41598-020-77979-y
Buran TJ, Sandhu AK, Li Z, Rock CR, Yang WW, Gu L (2014) Adsorption/desorption characteristics and separation of anthocyanins and polyphenols from blueberries using macroporous adsorbent resins. J Food Eng 128:167–173. https://doi.org/10.1016/j.jfoodeng.2013.12.029
Chen Y, Du X-J, Zhang Y, Liu X-H, Wang X-D (2017) Ultrasound extraction optimization, structural features, and antioxidant activity of polysaccharides from Tricholoma matsutake. J Zhejiang Univ Sci B 18(8):674–684. https://doi.org/10.1631/jzus.B1600239
Dandan K, Shaodan P, Jihua L, Yupo C (2018) Study on the effect of extraction process of moringa instant tea on its sensory quality. IOP Conf Ser Earth Environ Sci. https://doi.org/10.1088/1755-1315/153/2/022026
de Sousa Junior FC, de Araújo Padilha CE, Chibério AS, Ribeiro VT, Martins DRA, de Oliveira JA, de Macedo GR, dos Santos ES (2016) Modeling and simulation of breakthrough curves of recombinant 503 antigen using immobilized metal affinity expanded bed adsorption chromatography. Sep Purif Technol 164:34–40. https://doi.org/10.1016/j.seppur.2016.03.019
Fan Q, Li Y (2023) Enrichment of rosmarinic acid from Salvia przewalskii Maxim. leaves using macroporous resin: adsorption/desorption behavior, process optimization followed by scale-up. Ind Crops Prod 191:115931. https://doi.org/10.1016/j.indcrop.2022.115931
Feng JY, Li M, Zhao S, Zhang C, Yang ST, Qiao S, Tan WF, Qu HJ, Wang DY, Pu ZG (2018) Analysis of evolution and genetic diversity of sweetpotato and its related different polyploidy wild species I. trifida using RAD-seq. BMC Plant Biol 18(1):181. https://doi.org/10.1186/s12870-018-1399-x
Fu R, Wang Y, Yu F, Wu X, Gu Y, Chen W (2016) Optimization of the macroporous resin-based adsorption of apple polyphenol through response surface methodology. Toxicol Environ Chem 98(3–4):479–491. https://doi.org/10.1080/02772248.2015.1123490
Hossain MN, Sarker U, Raihan MS, Al-Huqail AA, Siddiqui MH, Oba S (2022) Influence of salinity stress on color parameters, leaf pigmentation, polyphenol and flavonoid contents, and antioxidant activity of Amaranthus lividus leafy vegetables. Molecules. https://doi.org/10.3390/molecules27061821
Huang H, Li Y, Gui F, Yang P, Zhang J, Li W, Zhong C, Cao L (2023) Optimizing the purification process of polyphenols of sea buckthorn seed and its potential freshness effect. LWT 173:114380. https://doi.org/10.1016/j.lwt.2022.114380
Jahan F, Bhuiyan MNH, Islam MJ, Ahmed S, Hasan MS, Bashera MA, Waliullah M, Chowdhury AN, Islam MB, Saha BK, Moulick SP (2022) Amaranthus tricolor (red amaranth), an indigenous source of nutrients, minerals, amino acids, phytochemicals, and assessment of its antibacterial activity. J Agric Food Res 10:100419. https://doi.org/10.1016/j.jafr.2022.100419
Li D, Xiao C (2019) Effects of temperature and pH on the physiological activity of grape juice 8(02):35–38+41. https://doi.org/10.19759/j.cnki.2164-4993.2019.02.009
Li J, Yang X, Zhao M, Wang L (2019) Study on the antioxidation of mango polyphenols. IOP Conf Ser Earth Environ Sci. https://doi.org/10.1088/1755-1315/332/3/032004
Liu Y, Liu J, Chen X, Liu Y, Di D (2010) Preparative separation and purification of lycopene from tomato skins extracts by macroporous adsorption resins. Food Chem 123(4):1027–1034. https://doi.org/10.1016/j.foodchem.2010.05.055
Liu Y, Kong KW, Wu D-T, Liu H-Y, Li H-B, Zhang J-R, Gan R-Y (2022) Pomegranate peel-derived punicalagin: ultrasonic-assisted extraction, purification, and its α-glucosidase inhibitory mechanism. Food Chem 374:131635. https://doi.org/10.1016/j.foodchem.2021.131635
Park JJ, Lee WY (2021) Adsorption and desorption characteristics of a phenolic compound from Ecklonia cava on macroporous resin. Food Chem 338:128150. https://doi.org/10.1016/j.foodchem.2020.128150
Sarker U, Oba S (2018) Drought stress enhances nutritional and bioactive compounds, phenolic acids and antioxidant capacity of Amaranthus leafy vegetable. BMC Plant Biol 18(1):258. https://doi.org/10.1186/s12870-018-1484-1
Sarker U, Oba S (2019) Antioxidant constituents of three selected red and green color Amaranthus leafy vegetable. Sci Rep 9(1):18233. https://doi.org/10.1038/s41598-019-52033-8
Sarker U, Oba S (2020) Nutraceuticals, phytochemicals, and radical quenching ability of selected drought-tolerant advance lines of vegetable amaranth. BMC Plant Biol 20(1):564. https://doi.org/10.1186/s12870-020-02780-y
Sarker U, Oba S, Ercisli S, Assouguem A, Alotaibi A, Ullah R (2022) Bioactive phytochemicals and quenching activity of radicals in selected drought-resistant Amaranthus tricolor vegetable amaranth. Antioxidants (basel). https://doi.org/10.3390/antiox11030578
Sarker U, Hossain MN, Oba S, Ercisli S, Marc RA, Golokhvast KS (2023) Salinity stress ameliorates pigments, minerals, polyphenolic profiles, and antiradical capacity in Lalshak. Antioxidants 12(1):173. https://doi.org/10.3390/antiox12010173
Singleton VL, Orthofer R, Lamuela-Raventós RM (1999) [14] Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. In: Methods in enzymology, vol 299. Academic Press, Cambridge, pp 152–178
Sun H, Mu B, Song Z, Ma Z, Mu T (2018) The in vitro antioxidant activity and inhibition of intracellular reactive oxygen species of sweet potato leaf polyphenols. Oxid Med Cell Longev 2018:9017828. https://doi.org/10.1155/2018/9017828
Sun N, Xie J, Xie J, Chen Y, Hu X, Yu Q (2022) Purification, composition and activity of bound polyphenols from mung bean coat dietary fiber. Food Res Int 162:111997. https://doi.org/10.1016/j.foodres.2022.111997
Tang W, Yao W, Lei X, Zhou Y, Liu M, Zeng Z, Zhao C (2023) Efficient enrichment and purification of anti-inflammatory nuezhenoside G13 from Osmanthus fragrans fruit by macroporous resin adsorption. Ind Crops Prod 192:116092. https://doi.org/10.1016/j.indcrop.2022.116092
Wan R, Liu J, Yang Z, Zhu P, Cao Q, Xu T (2020) Genome-wide identification, characterisation and expression profile analysis of DEAD-box family genes in sweet potato wild ancestor Ipomoea trifida under abiotic stresses. Genes Genomics 42(3):325–335. https://doi.org/10.1007/s13258-019-00910-x
Xi L, Mu T, Sun H (2015) Preparative purification of polyphenols from sweet potato (Ipomoea batatas L.) leaves by AB-8 macroporous resins. Food Chem 172:166–174. https://doi.org/10.1016/j.foodchem.2014.09.039
Xiaoting W, Kangmingli, Lijun S, Jiayin Y (2022) Purification of black corn anthocyanins by macroporous adsorption resin. Food Ind 43(05):19–24
Yan P, Kehua T, Aiwen D (2019) Purification of polyphenols from rhizoma bletillariae by macroporous resin and its antioxidant activity in vitro. For Prod Chem Ind 39(04):91–99
Zhang C, Liu D, Wu L, Zhang J, Li X, Wu W (2020) Chemical characterization and antioxidant properties of ethanolic extract and its Fractions from sweet potato (Ipomoea batatas L.) leaves. Foods https://doi.org/10.3390/foods9010015
Zhang L, Yong YY, Deng L, Wang J, Law BY, Hu ML, Wu JM, Yu L, Wong VK, Yu CL, Qin DL (2023) Therapeutic potential of polygala saponins in neurological diseases. Phytomedicine 108:154483. https://doi.org/10.1016/j.phymed.2022.154483
Funding
Fund projects: Shandong Vegetable Industry Technology System (SDAIT-05); The scientific research project of young teachers of Shandong Agriculture and Engineering University, "Extraction, component analysis and antioxidant activity of polyphenols in foods" (project number: QNKZY201902); "Postharvest quality control and comprehensive utilization of characteristic agricultural products", the key laboratory of Shandong Provincial Department of Education.
Author information
Authors and Affiliations
Contributions
All authors have contributed to the conception and design of the study. All authors collected and analyzed data. All authors commented on the previous version of the manuscript. All authors have read and approved the final manuscript.
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no competing financial interests.
Ethical approval
Not applicable.
Consent to participate
Not applicable.
Consent for publication
Not applicable.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Yang, LF., Nie, W., Cui, YP. et al. Purification with macroporous resin and antioxidant activity of polyphenols from sweet potato leaves. Chem. Pap. 78, 181–188 (2024). https://doi.org/10.1007/s11696-023-03058-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11696-023-03058-x