Abstract
Cancer is the second foremost cause of death worldwide, and despite modern medicine development, it is needed to develop new plant-based drugs. The current study is mainly focused on the estimation of anticancerous activity of silver nanoparticles (AgNPs) fabricated by a simple and eco-friendly green approach by using leaf extract of the medicinal plant Blumea lacera (B. lacera). The prepared AgNPs were characterized by using different analytical tools. UV–visible spectra were recorded, which exhibited a sharp surface plasmonic resonance (SPR) band at 430 nm and confirmed the formation of AgNPs. The spherical morphology of synthesized AgNPs was determined with the help of field emission scanning electron microscopy (FE-SEM) and transmission electron microscopy (TEM), and the average particle size was calculated using TEM and found to be 12.52 nm. The Fourier transformed infrared (FT-IR) spectrum of AgNPs showed characteristic bands of functional groups present in the biomolecules adsorb onto AgNPs, acting as a stabilizing agent. The crystallite nature of AgNPs formed was confirmed by the powder X-ray diffraction (PXRD) technique. The anticancerous activity of synthesized AgNPs was investigated against adherent human lung carcinoma cell A549. The minimal inhibition concentration (IC50 or MIC) value was found to be ~ 20 μg/mL for human lung carcinoma cell A549, and the result so obtained suggests that synthesized AgNPs via B. lacera possess a good ability to be used as an eco-friendly anticancerous agent. Moreover, the synthesized AgNPs possess good antioxidant activity compared to B. lacera plant leaves. The minimal inhibition concentration (IC50) of ~ 6 μg/mL for synthesized AgNPs was found.
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Abbreviations
- AgNPs:
-
Silver nanoparticles
- NPs:
-
Nanoparticles
- B. lacera:
-
Blumea lacera
- Fig.:
-
Figure
- FT-IR:
-
Fourier transformed infrared
- UV–Vis:
-
Ultraviolet–visible
- FE-SEM:
-
Field emission scanning electron microscopy
- DLS:
-
Dynamic light scattering
- EDX:
-
Energy-dispersive X-ray spectroscopy
- TEM:
-
Transmission electron microscopy
- PXRD:
-
Powder X-ray diffraction
- Eq.:
-
Equation
- MTT:
-
3-(4,5-Dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide tetrazolium salt
- DPPH:
-
2,2-Diphenylpicryl-1-hydrazyl
- SPR:
-
Surface plasmon resonance
References
Abdel-Aziz MS, Shaheen MS, EI-Nekeety AA, Abdel-Wahhab MA (2013) Antioxidant and antibacterial activity of silver nanoparticles using Chenopodium murale leaf extract. J Saudi Chem Soc 18:356–363. https://doi.org/10.1016/j.jscs.2013.09.011
Adewale OB, Egbeyemi KA, Onwuelu JO, Potts-Johnson SS, Anadozie SO, Fadaka AO, Osukoya OA, Aluko BT, Johnson J, Obafemi TO (2020) Biological synthesis of gold and silver nanoparticles using leaf extracts of Crassocephalum rubens and their comparative in vitro antioxidant activities. Heliyon 6:e05501. https://doi.org/10.1016/j.heliyon.2020.e05501
Ahmed FA, Rahman A, Mubassara S (2014) Phytochemical composition, antioxidant activity and cytotoxicity of Blumea lacera Linn. from two different habitats. Jahangirnagar Univ J Biol Sc 3:37–45. https://doi.org/10.3329/jujbs.v3i1.28276
Alahmad A, Feldhoff A, Bigall NC, Rusch P, Scheper T, Walter JG (2021) Hypericum perforatum L.-mediated green synthesis of silver nanoparticles exhibiting antioxidant and anticancer activities. Nanomaterials 11:487. https://doi.org/10.3390/nano11020487
AlNadhari S, Al-Enazi N, Alshehrei F, Ameen F (2020) A review on biogenic synthesis of metal nanoparticles using marine algae and its applications. Environ Res 194:110672. https://doi.org/10.1016/j.envres.2020.110672
Ameen F, Srinivasan P, Selvankumar T, Kamala-Kannan S, Al Nadhari S, Almansob A, Dawoud T, Govarthanan M (2019) Phytosynthesis of silver nanoparticles using Mangifera indica flower extract as bioreductant and their broad-spectrum antibacterial activity. Bioorgan Chem 88:102970. https://doi.org/10.1016/j.bioorg.2019.102970
Anandalakshmi K, Venugobal J, Ramasamy V (2016) Characterization of silver nanoparticles by green synthesis method using Pedalium murex leaf extract and their antibacterial activity. Appl Nanosci 6:399–408. https://doi.org/10.1007/s13204-015-0449-z
Aziz SB (2017) Investigation of metallic silver nanoparticles through UV–Vis and optical micrograph techniques. Int J Electrochem Sci. https://doi.org/10.20964/2017.01.22
Badán JA, Navarrete-Astorga E, Henriquez R, Martin F, Marotti RE, Ramos-Barrado JR, Dalchiele EA (2020) Optical properties of silver nanoparticles deposited onto silicon substrates by different soft-solution processing techniques. Opt Mater 100:1096651. https://doi.org/10.1016/j.optmat.2020.109651
BalaKumaran M, Ramachandran R, Balashanmugam P, Jagadeeswari S, Kalaichelvan PJMT (2022) Comparative analysis of antifungal, antioxidant and cytotoxic activities of mycosynthesized silver nanoparticles and gold nanoparticles. Mater Technol 37:411–421. https://doi.org/10.1080/10667857.2020.1854518
Braga LR, Rangel ET, Suarez PAZ, Machado F, Life S (2018) Simple synthesis of active films based on PVC incorporated with silver nanoparticles: evaluation of the thermal, structural and antimicrobial properties. Food Packag Shelf Life 15:122–129. https://doi.org/10.1016/j.fpsl.2017.12.005
Calderón-Jiménez B, Montoro Bustos AR, Pereira Reyes R, Paniagua SA, Vega-Baudrit JR (2022) Novel pathway for the sonochemical synthesis of silver nanoparticles with near-spherical shape and high stability in aqueous media. Sci Rep 12:1–17. https://doi.org/10.1038/s41598-022-04921-9
Gangwar C, Yaseen B, Kumar I, Singh NK, Naik RM (2021) Growth kinetic study of tannic acid mediated monodispersed silver nanoparticles synthesized by chemical reduction method and its characterization. ACS Omega 6:22344–22356. https://doi.org/10.1021/acsomega.1c03100
Gangwar C, Yaseen B, Nayak R, Praveen S, Singh NK, Sarkar J, Banerjee M, Naik RM (2022) Silver nanoparticles fabricated by tannic acid for their antimicrobial and anticancerous activity. Inorg Chem Commun 141:109532. https://doi.org/10.1016/j.inoche.2022.109532
Gangwar C, Yaseen B, Nayak R, Baker A, Bano N, Singh NK, Naik RM (2023) Madhuca longifolia leaves-mediated palladium nanoparticles synthesis via a sustainable approach to evaluate its biomedical application. Chem Pap. https://doi.org/10.1007/s11696-023-02688-5
Garibo D, Borbón-Nuñez HA, de León JND et al (2020) Green synthesis of silver nanoparticles using Lysiloma acapulcensis exhibit high-antimicrobial activity. Sci Rep 10:12805. https://doi.org/10.1038/s41598-020-69606-7
Guilger-Casagrande M, Germano-Costa T, Bilesky-José N, Pasquoto-Stigliani T, Carvalho L, Fraceto LF, de Lima R (2021) Influence of the capping of biogenic silver nanoparticles on their toxicity and mechanism of action towards Sclerotinia sclerotiorum. J Nanobiotechnol 19:1–18. https://doi.org/10.1186/s12951-021-00797-5
Htwe Y, Abdullah M, Mariatti M (2022) Water-based graphene/AgNPs hybrid conductive inks for flexible electronic applications. J Mater Res Technol 16:59–73. https://doi.org/10.1016/j.jmrt.2021.11.159
Hussein HA, Abdullah MA (2021) Novel drug delivery systems based on silver nanoparticles, hyaluronic acid, lipid nanoparticles and liposomes for cancer treatment. Appl Nanosci. https://doi.org/10.1007/s13204-021-02018-9
Jadhav K, Dhamecha D, Bhattacharya DD, Patil M (2016) Green and ecofriendly synthesis of silver nanoparticles: characterization, biocompatibility studies and gel formulation for treatment of infections in burns. J Photochem Photobiol B: Biol. https://doi.org/10.1016/j.jphotobiol.2016.01.002
Jadhav K, Deore S, Dhamecha DHRR, Jagwani S, Jalalpure S, Bohara R (2018) Phytosynthesis of silver nanoparticles: characterization, biocompatibility studies, and anticancer activity. ACS Biomater Sci Eng. https://doi.org/10.1021/acsbiomaterials.7b00707
Jagwani S, Jalalpure S, Dhamecha D, Jadhav K, Bohara R (2020) Pharmacokinetic and pharmacodynamic evaluation of resveratrol loaded cationic liposomes for targeting hepatocellular carcinoma. ACS Biomater Sci Eng. https://doi.org/10.1021/acsbiomaterials.0c00429
Jha M, Shimpi NG (2018) Green synthesis of zero valent colloidal nanosilver targeting A549 lung cancer cell: in vitro cytotoxicity. J Genetic Eng Biotechnol 16:115–124. https://doi.org/10.1016/j.jgeb.2017.12.001
Karthik P, Singh SP (2015) Conductive silver inks and their applications in printed and flexible electronics. RSC Adv 5:77760–77790. https://doi.org/10.1039/C5RA12013F
Khalir WKAWM, Shameli K, Jazayeri SD, Othman NA, Jusoh NWC, Hassan NM (2020) Biosynthesized silver nanoparticles by aqueous stem extract of Entada spiralis and screening of their biomedical activity. Front Chem 8:620. https://doi.org/10.3389/fchem.2020.00620
Khatoon UT, Rao GN, Mohan KM, Ramanaviciene A, Ramanavicius A (2017) Antibacterial and antifungal activity of silver nanospheres synthesized by tri-sodium citrate assisted chemical approach. Vacuum 146:259–265. https://doi.org/10.1016/j.vacuum.2017.10.003
Kumar I, Gangwar C, Yaseen B, Pandey PK, Mishra SK, Naik RM (2022a) Kinetic and mechanistic studies of the formation of silver nanoparticles by nicotinamide as a reducing agent. ACS Omega 7:13778–13788. https://doi.org/10.1021/acsomega.2c00046
Kumar I, Nayak R, Chaudhary LB, Pandey VN, Mishra SK, Singh NK, Srivastava A, Prasad AS, Naik RM (2022b) Fabrication of α-Fe2O3 nanostructures: synthesis, characterization, and their promising application in the treatment of carcinoma A549 lung cancer cells. ACS Omega 25:21882–21890. https://doi.org/10.1021/acsomega.2c02083
Labulo AH, David OA, Terna AD (2022) Green synthesis and characterization of silver nanoparticles using Morinda lucida leaf extract and evaluation of its antioxidant and antimicrobial activity. Chem Pap. https://doi.org/10.1007/s11696-022-02392-w
Lugani Y, Sooch BS, Singh P, Kumar S (2021) Nanobiotechnology applications in food sector and future innovations. Microbial biotechnology in food and health. Elsevier, pp 197–225. https://doi.org/10.1016/B978-0-12-819813-1.00008-6
Mamdouh S, Mahmoud A, Samir A, Mobarak M, Mohamed T (2022) Using femtosecond laser pulses to investigate the nonlinear optical properties of silver nanoparticles colloids in distilled water synthesized by laser ablation. Physica B: Condensed Matter 631:413727. https://doi.org/10.1016/j.physb.2022.413727
Mendhulkar VD, Patade P, Vakil M (2016) Elicitation of flavonoids in Blumea lacera (Burm.f.) DC. Cell culture using chemical elicitor, salicylic acid and biological elicitor, aspergillus Niger. Int J Curr Res Biosci Plant Biol 3(11):85–91. https://doi.org/10.20546/ijcrbp.2016.311.013
Mishra SK, Tripathi U, Awasthi RR, Shukla R, Kumar I, Naik RM, Mishra D (2021) CTAB mediated synthesis of ZnO nanoparticles: Structural, optical and enhanced blue-green optical emission. Mater Today: Proc 46:2229–2234. https://doi.org/10.1016/j.matpr.2021.03.481
Mistry H, Thakor R, Patil C, Trivedi J, Bariya H (2020) Biogenically proficient synthesis and characterization of silver nanoparticles employing marine procured fungi Aspergillus brunneoviolaceus along with their antibacterial and antioxidative potency. Biotechnol Lett 43:307–316. https://doi.org/10.1007/s10529-020-03008-7
Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63. https://doi.org/10.1016/0022-1759(83)90303-4
Paaniappan P, Satishkumar G, Sankar R (2015) Fabrication of nano-silver particles using Cymodocea serrulata and its cytotoxicity effect against human lung cancer A549 cells line. Spectro Acta Part A Mol Biomol Spectrosc 138:885–890. https://doi.org/10.1016/j.saa.2014.10.072
Padmini R, Nallal UM, Razia M, Sivaramakrishnan S, Alodaini HA, Hatamleh AA, AL-Dosary MA, Rangnathan V, Chung WJ (2022) Cytotoxic effect of silver nanoparticles synthesized from ethanolic extract of Allium sativum on A549 lung cancer cell line. J King Saud Univ Sci. https://doi.org/10.1016/j.jksus.2022.102001
Pulit-Prociak J, Grabowska A, Chwastowski J, Majka TM, Banach M (2019) Safety of the application of nanosilver and nanogold in topical cosmetic preparations. Coll Surf B: Biointerfaces 183:110416. https://doi.org/10.1016/j.colsurfb.2019.110416
Ratan ZA, Haidere MF, Nurunnabi M, Shahriar SM, Ahammad AJS, Shim YY, Reaney MJT, Cho JY (2020) Green chemistry synthesis of silver nanoparticles and their potential anticancer effects. Cancers (basel) 12:885. https://doi.org/10.3390/cancers12040855
Ravichandrana V, Vasanthi S, Shalini S, Shah SAA, Haris R (2016) Green synthesis of silver nanoparticles using Atrocarpus altilis leaf extract and the study oftheir antimicrobial and antioxidant activity. Mater Lett 180:264–267. https://doi.org/10.1016/j.matlet.2016.05.172
Sankar R, Karthik A, Prabu A, Karthik S, Shivashangari K S, Ravikumar V (2013) Origanum vulgare mediated biosynthesis of silver nanoparticles for its antibacterial and anticancer activity. Coll Surf B: Biointerfaces 108:80–84. https://doi.org/10.1016/j.colsurfb.2013.02.033
Selvan DA, Mahendiran D, Kumar RS, Rahiman AK (2018) Garlic, green tea and turmeric extracts-mediated green synthesis of silver nanoparticles: phytochemical, antioxidant and in vitro cytotoxicity studies. J Photochem Photobiol, B 180:243–252. https://doi.org/10.1016/j.jphotobiol.2018.02.014
Shahcheraghi N, Golchin H, Sadri Z, Tabari Y, Borhanifar F, Makani S (2022) Nano-biotechnology, an applicable approach for sustainable future. Biotech 12:65. https://doi.org/10.1007/s13205-021-031089
Shalini A, Priya K, Kothai S, Kannaiyan P, Anbalagan G, Jaisankar V (2022) Synthesis and characterisation of graphene oxide decorated gold nano particles and their application towards antibacterial activity. Chem Pap. https://doi.org/10.1007/s11696-022-02375-x
Sharma D, Kanchi S, Bisetty K (2015) Biogenic synthesis of nanoparticles: a review. Arab J Chem 12:3576–3600. https://doi.org/10.1016/j.arabjc.2015.11.002
Shen L, Chen M, Hu L, Chen X, Wang J (2013) Growth and stabilization of silver nanoparticles on carbon dots and sensing application. Langmuir 29:16135–16140. https://doi.org/10.1021/la404270w
Singh I, Al-Wahaibi LH, Srivastava R, Prasad O, Pathak SK, Kumar S, Parveen S, Banerjee M, El-Emam AA, Sinha L (2020) DFT study on the electronic properties, spectroscopic profile, and biological activity of 2-Amino-5-trifluoromethyl-1,3,4-thiadiazole with anticancer properties. ACS Omega 5(46):30073–30087. https://doi.org/10.1021/acsomega.0c04474
Sreelekha E, George B, Shyam A, Sajina N, Mathew B (2021) A comparative study on the synthesis, characterization, and antioxidant activity of green and chemically synthesized silver nanoparticles. BioNanoScience 11:489–496. https://doi.org/10.1007/s12668-021-00824-7
Sytu MRC, Camacho DH (2018) Green synthesis of silver nanoparticles (AgNPs) from Lenzites betulina and the potential synergistic effect of AgNPs and capping biomolecules in enhancing antioxidant activity. BioNanoScience 8:835–844. https://doi.org/10.1007/s12668-018-0548-x
Thampi N, Shalini VJ (2015) Bio-prospecting the in-vitro antioxidant and anti-cancer activities of silver nanoparticles synthesized from the leaves of syzygium samarangense. Int J Pharm Pharm Sci 7:269–274
Venkatesan B, Subramanian V, Tumala A, Vellaichamy E (2014) Rapid synthesis of biocompatible silver nanoparticles using aqueous extract of Rosa damascena petals and evaluation of their anticancer activity. Asian Pac J Trop Med 7S1:S294-300. https://doi.org/10.1016/s1995-7645(14)60249-2
Wu J, Zhao N, Zhang X, Xu J (2012) Cellulose/silver nanoparticles composite microspheres: eco-friendly synthesis and catalytic application. Cellulose 19:1239–1249. https://doi.org/10.1007/s10570-012-9731-3
Xu S, Zhu Q, Lin X, Lin W, Qin Y, Li Y (2020) The phase behavior of n-ethylpyridinium tetrafluoroborate and sodium-based salts ATPS and its application in 2-chlorophenol extraction Chinese. J Chem Eng. https://doi.org/10.1016/j.cjche.2020.07.024
Yadav P, Pandey SK, Shama P, Kumar S, Banerjee M, Sethi A (2021) Experimental and theoretical investigation of synthesized pregnenolone derivatives via palladium catalyzed cross coupling reactions, their anticancer activity against lung cancer cells. J Mol Struct 1245:131115. https://doi.org/10.1016/j.molstruc.2021.131115
Yaseen B, Gangwar C, Kumar I, Sarkar J, Naik RM (2022) Detailed kinetic and mechanistic study for the preparation of silver nanoparticles by a chemical reduction method in the presence of a neuroleptic agent (Gabapentin) at an Alkaline pH and its characterization. ACS Omega 7:5739–5750. https://doi.org/10.1021/acsomega.1c05499
Zhu Q, Xu S, Wu W, Qi Y, Lin Z, Li Y, Qin Y (2022) Hierarchical hollow zinc oxide nanocomposites derived from morphology-tunable coordination polymers for enhanced solar hydrogen production. Angewandte Chemie Int Edition. https://doi.org/10.1002/anie.202205312
Acknowledgements
The authors are highly grateful to the Head of Department (HoD), Department of Chemistry (DoC), University of Lucknow (UoL), Lucknow (UP), India, for providing basic infrastructure facilities, like UV–visible spectrophotometry and FT-IR spectroscopy for performing experimental work for my Ph.D. degree. The authors are also thankful to Vice-Chancellor Prof. Alok Kumar Rai of Lucknow University for partially assisting with funds through the Award of Chhatra Kalyan scholarship to PP funded by University of Lucknow, India. The authors are also grateful to the Advanced Materials Research Centre (AMRC), Kamand campus, IIT Mandi, H.P, the sophisticated analytical instrumentation facility (SAIF), All India Institute of Medical Science (AIIMS), New Delhi, and Birbal Sahni institute of Palaeosciences, Lucknow, UP, India, for providing X-ray diffraction, transmission electron microscopy and field emission scanning electron microscopy facilities, respectively. The authors are indebted to the Centre of Excellence scheme, Government of Uttar Pradesh, India, for cell culture facility in Molecular and Human Genetics Laboratory, Department of Zoology, University of Lucknow, Lucknow.
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Pandey, P.K., Gangwar, C., Yaseen, B. et al. Anticancerous and antioxidant properties of fabricated silver nanoparticles involving bio-organic framework using medicinal plant Blumea lacera. Chem. Pap. 77, 3603–3617 (2023). https://doi.org/10.1007/s11696-023-02723-5
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DOI: https://doi.org/10.1007/s11696-023-02723-5