Skip to main content
Log in

Lab-scale testing and evaluation of microbes' ability to reduce oil viscosity

  • Original Paper
  • Published:
Chemical Papers Aims and scope Submit manuscript

Abstract

Microbial enhanced oil recovery (MEOR) is a tertiary oil recovery method which involves the injection of carefully screened microorganisms into the formation resulting in in situ fermentation to improve oil recovery performance. Improvement in the recovery of oil with the help of microbes could be achieved through several efficient mechanisms mainly through interfacial tension and wettability reduction. This research is performed on a laboratory scale, focused on crude oil samples screening, taken from shallow reservoirs with lower API gravity to study the impact of microbes on the viscosity reduction to cause the degradation of heavy oil into light oil. The crude from the reservoir can be recovered to the surface using driving forces that can be evolved due to the by-product gases of microbes and the resultant elevated reservoir pressure.

In this study, the fusing and mixing of different bacteria strains are successfully conducted on multiple crude oil samples, with API gravity of 39, 34, and 30, while a sample of heavy crude oil with API gravity of 14. Detailed laboratory investigation is performed and results are discussed. The laboratory results demonstrate that viscosity of crude sample is reduced which contributes positively in oil recovery. Compared to other tertiary oil recovery methods, MEOR can be an economical and environmentally friendly alternative depending on the understanding of the reservoir physics and the chemistry of the injected microbe’s response which is found to be different for every individual crude oil sample.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  • Amro Mohammed M (2008) Multidisciplinary challenge for microbial enhanced oil recovery (MEOR). In SPE Saudi Arabia section technical symposium, OnePetro

    Google Scholar 

  • Aziz H, Muther T, Khan MJ, Syed FI (2021) A review on nanofluid water alternating gas (N-WAG): application, preparation, mechanism, and challenges. Arab J Geosci 14(14):1–12

    Article  Google Scholar 

  • Beckman JW (1926) "Action of bacteria on mineral oil."Ind Eng Chem., News Ed. 4, no. 21 : 3.

  • Behlülgil K, Mehmetoğlu MT (2002) Bacteria for improvement of oil recovery: a laboratory study. Energy Sources 24(5):413–421

    Article  Google Scholar 

  • Bryant RS, Douglas J (1988) Evaluation of microbial systems in porous media for EOR. SPE Reserv Eng 3(02):489–495

    Article  CAS  Google Scholar 

  • Chang PL, Yen TFu (1984) Interaction of Escherichia coli B and B/4 and bacteriophage T4D with Berea sandstone rock in relation to enhanced oil recovery. Appl Environ Microbiol 47(3):544–550

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Cheng M, Long Yu, Gao J, Lei G, Zhang Z (2021) Isolating, identifying and evaluating of oil degradation strains for the air-assisted microbial enhanced oil recovery process. Plos One 16(1):e0243976

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Gray M, Yeung A, Foght J, Yarranton HW (2008) Potential microbial enhanced oil recovery processes: a critical analysis. In SPE annual technical conference and exhibition, OnePetro

    Google Scholar 

  • Hamme V, Jonathan D, Singh A, Ward OP (2003) Recent advances in petroleum microbiology. Microbiol Mol Biol Rev 67(4):503–549

    Article  PubMed  PubMed Central  Google Scholar 

  • He J, Y Wang, G Liang (2018) "Emerging strategic technology of the oilfield development."

  • Hitzman DO"Review of microbial enhanced oil recovery field tests."In: Proceedings of the applications of microorganisms to petroleum technology (1988)

  • Hou Zhaowei, Han Peihui, Le Jianjun, Chang Jianfei, Dou Xumou, Guo Menghua, Chen Xinghong (2008) The application of hydrocarbon-degrading bacteria in Daqing’s low permeability, high paraffin content oilfields. In SPE Symposium on Improved Oil Recovery, OnePetro

    Google Scholar 

  • Jimoh, IA, Rudyk SN, Søgaard EG (2011) "Microbial enhanced oil recovery-laboratory experiments with a strain of Clostridium tyrobutyricum." In: 1st European studen conference on microbial communication

  • Ke C-Y, Sun W-J, Li Y-B, Hui J-F, Guo-Min Lu, Zheng X-Y, Zhang Q-Z, Zhang X-L (2018) Polymer-assisted microbial-enhanced oil recovery. Energy Fuels 32(5):5885–5892

    Article  CAS  Google Scholar 

  • Khire JM, Khan MI (1994) Microbially enhanced oil recovery (MEOR). I: importance and mechanism of MEOR. Enzyme Microb Technol 16(2):170–172

    Article  CAS  Google Scholar 

  • Kryachko Y (2018) Novel approaches to microbial enhancement of oil recovery. J Biotechnol 266:118–123

    Article  CAS  PubMed  Google Scholar 

  • Lan G, Fan Q, Yongqiang Liu Yu, Liu YL, Yin X, Luo M (2015) Effects of the addition of waste cooking oil on heavy crude oil biodegradation and microbial enhanced oil recovery using Pseudomonas sp. SWP-4. Biochem Eng J 103:219–226

    Article  CAS  Google Scholar 

  • Lazar I, Petrisor IG, Yen TF (2007) Microbial enhanced oil recovery (MEOR). Pet Sci Technol 25(11):1353–1366

    Article  CAS  Google Scholar 

  • McInerney MJ, Duncan KE, Youssef N, Fincher T, Maudgalya SK, Folmsbee MJ, Knapp R, Simpson RR, Ravi N, Nagle D (2005) Development of microorganisms with improved transport and biosurfactant activity for enhanced oil recovery. Univ. of Oklahoma, Norman, OK (United States)

    Book  Google Scholar 

  • Muther T, Qureshi HA, Syed FI, Aziz H, Siyal A, Dahaghi AK, Negahban S (2021) Unconventional hydrocarbon resources: geological statistics, petrophysical characterization, and field development strategies. J Pet Explor Prod Technol 12:1–26

    Google Scholar 

  • Nielsen SM, Shapiro AA, Michelsen ML, Stenby EH (2010) 1D simulations for microbial enhanced oil recovery with metabolite partitioning. Transp Porous Media 85(3):785–802

    Article  CAS  Google Scholar 

  • Nielsen SM, Shapiro A, Stenby EH, Michelsen ML (2011) Microbial enhanced oil recovery: advanced reservoir simulation. Center for Energy Resources Engineering Technical University of Denmark, Denmark

    Google Scholar 

  • Niu J, Liu Qi, Lv J, Peng Bo (2020) Review on microbial enhanced oil recovery: mechanisms, modeling and field trials. J Petrol Sci Eng 192:107350

    Article  CAS  Google Scholar 

  • Nnaemeka O, Franklin N, Stanley O (2018) A review of microbial enhanced oil recovery applications projects. Oil Gas Res 4(152):2472–518

    Google Scholar 

  • O’Bryan OD, Ling TWD (1949) The effect of the bacteria Vibrio desulfuricans on the permeability of limestone cores. Texas J Sci 1:117–128

    Google Scholar 

  • Patel J, Borgohain S, Kumar M, Rangarajan V, Somasundaran P, Sen R (2015) Recent developments in microbial enhanced oil recovery. Renew Sustain Energy Rev 52:1539–1558

    Article  CAS  Google Scholar 

  • Pereira JFB, Gudiña EJ, Costa R, Vitorino R, Teixeira JA, Coutinho JAP, Rodrigues LR (2013) Optimization and characterization of biosurfactant production by Bacillus subtilis isolates towards microbial enhanced oil recovery applications. Fuel 111:259–268

    Article  CAS  Google Scholar 

  • Quraishi M, Bhatia SK, Pandit S, Gupta PK, Rangarajan V, Lahiri D, Varjani S, Mehariya S, Yang Y-H (2021) Exploiting microbes in the petroleum field: analyzing the credibility of microbial enhanced oil recovery (MEOR). Energies 14(15):4684

    Article  CAS  Google Scholar 

  • Rellegadla S, Prajapat G, Agrawal A (2017) Polymers for enhanced oil recovery: fundamentals and selection criteria. Appl Microbiol Biotechnol 101(11):4387–4402

    Article  CAS  PubMed  Google Scholar 

  • Safdel M, Anbaz MA, Daryasafar A, Jamialahmadi M (2017) Microbial enhanced oil recovery, a critical review on worldwide implemented field trials in different countries. Renew Sust Energy Rev 74:159–172

    Article  CAS  Google Scholar 

  • Saravanan A, Kumar PS, Vardhan KH, Jeevanantham S, Karishma SB, Yaashikaa PR, Vellaichamy P (2020) A review on systematic approach for microbial enhanced oil recovery technologies: Opportunities and challenges. J Clean Product 258:120777

    Article  CAS  Google Scholar 

  • Sen R (2008) Biotechnology in petroleum recovery: the microbial EOR. Prog Energy Combust Sci 34(6):714–724

    Article  CAS  Google Scholar 

  • Singh NK, Choudhary S (2021) Bacterial and archaeal diversity in oil fields and reservoirs and their potential role in hydrocarbon recovery and bioprospecting. Env Sci Pollut Res 28(42):58819–58836

    Article  CAS  Google Scholar 

  • Song Z, Zhu W, Sun G, Blanckaert K (2015) Dynamic investigation of nutrient consumption and injection strategy in microbial enhanced oil recovery (MEOR) by means of large-scale experiments. Appl Microbiol Biotechnol 99(15):6551–6561

    Article  CAS  PubMed  Google Scholar 

  • Suthar H, Hingurao K, Desai A, Nerurkar A (2009) Selective plugging strategy based microbial enhanced oil recovery using Bacillus licheniformis TT33. J Microbiol Biotechnol 19(10):1230–1237

    CAS  PubMed  Google Scholar 

  • Syed FI, Negahban S, Dahaghi AK (2021) Infill drilling and well placement assessment for a multi-layered heterogeneous reservoir. J Pet Explor Prod 11(2):901–910

    CAS  Google Scholar 

  • Syed FI, Dahaghi AK, Muther T (2022) Laboratory to field scale assessment for EOR applicability in tight oil reservoirs. Pet Sci. https://doi.org/10.1016/j.petsci.2022.04.014

    Article  Google Scholar 

  • Syed FI, Muther T, Van VP, Dahaghi AK, Negahban S (2022) Numerical trend analysis for factors affecting EOR performance and CO2 storage in tight oil reservoirs. Fuel 316:123370

    Article  CAS  Google Scholar 

  • Syed FI, Muther T, Dahaghi AK, Neghabhan S (2022) CO2 EOR performance evaluation in an unconventional reservoir through mechanistic constrained proxy modeling. Fuel 310:122390

    Article  CAS  Google Scholar 

  • Updegraff, David M. (1983) "Plugging and penetration of petroleum reservoir rock by microorganisms." In Proceedings of the 1982 International Conference on Microbial Enhancement of Oil Recovery, vol. 80, p. 85. Shangri-La, Afton, Oklahoma. B. Linville, Bartlesville Energy Technology Center, Bartlesville, OK

  • Xia W, Shen W, Li Yu, Zheng C, Weichu Yu, Tang Y (2016) Conversion of petroleum to methane by the indigenous methanogenic consortia for oil recovery in heavy oil reservoir. Appl Energy 171:646–655

    Article  CAS  Google Scholar 

  • Yen TF (1989) "Microbial enhanced oil recovery"

  • Zhang X, Xiang T (2010) Review on microbial enhanced oil recovery technology and development in China. Int J Pet Sci Technol 4(1):61–81

    Google Scholar 

  • Zhang J, Gao H, Xue Q (2020) Potential applications of microbial enhanced oil recovery to heavy oil. Crit Rev Biotechnol 40(4):459–474

    Article  CAS  PubMed  Google Scholar 

  • ZoBell C (1947) Bacterial release of oil from oil-bearing materials. World oil 127:35–41

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Muhammad Murtaza.

Ethics declarations

Conflict of interest

On behalf of all authors, the corresponding author states that there is no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Murtaza, M., Khan, I., Muther, T. et al. Lab-scale testing and evaluation of microbes' ability to reduce oil viscosity. Chem. Pap. 77, 1869–1878 (2023). https://doi.org/10.1007/s11696-022-02581-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11696-022-02581-7

Keywords

Navigation