Abstract
This study focuses on the synthesize of magnetic nanoparticles (MNPs) that can be used practically in the separation and purification processes of DNA. For this purpose, firstly, bilayer oleic acid coated Fe3O4 (magnetite:M) nanoparticles with high water dispersibility and dispersion stability were synthesized. Subsequently, various amounts of acrylamide and crosslinker N, N-methylenebisacrylamide (NMBA) were added to the aqueous solution containing 4% by weight of magnetic particles, and the surface of the magnetic particles was coated with a cross-linked polyacrylamide (pAAm) by using reverse emulsion polymerization technique. Finally, these pAAm coated particles were modified by transamidation reaction with ethylene diamine yielding aminofunctional core–shell structure MNPs. The synthesized MNPs were characterized by various tests and techniques such as amine value (AV), FTIR, XRD, DLS, and TGA. The results showed that lowest particle size, narrow size distribution, and highest AV without any significant loss of magnetic properties can be obtained after EDA modification when acrylamide mole amount is fixed to 10–2 mol in the polymerization reaction (particle size = 180 nm; AV:19.53 mmol/g). Finally, the DNA isolation studies showed that 4.5 μg/mg DNA adsorption capacity can be achieved at pH4 in 30 min; while 80% of the adsorbed DNA can be desorbed at pH 10 in 10 min. The obtained results indicated the effectiveness and convenience of the synthesized MNPs in biotechnology and DNA separation processes.
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Abbreviations
- Fe3O4 :
-
M
- NP:
-
Nano particle
- DNA:
-
Deoxyribonucleic acid
- RNA:
-
Ribonucleic acid
- OA:
-
Oleic acid
- pAAm:
-
Polyacrylamide
- EDA:
-
Ethylenediamine
- AV:
-
Amine value
- PVA:
-
Poly(vinyl alcohol)
- NMBA:
-
N,N'-Methylenebisacrylamide
References
Abakumov MA, Semkina AS, Skorikov AS, Vishnevskiy DA, Ivanova AV, Mironova E, Davydova GA, Majouga AG, Chekhonin VP (2018) Toxicity of iron oxide nanoparticles: size and coating effects. J Biochem Mol Toxicol 32:1–6. https://doi.org/10.1002/jbt.22225
Ansari SA, Husain Q (2012) Potential applications of enzymes immobilized on/in nano materials: a review. Biotechnol Adv 30:512–523. https://doi.org/10.1016/j.biotechadv.2011.09.005
Baharuddin AA, Ang BC, Abu Hussein NA, Andriyana A, Wong YH (2018) Mechanisms of highly stabilized ex-situ oleic acid-modified iron oxide nanoparticles functionalized with 4-pentynoic acid. Mater Chem Phys. https://doi.org/10.1016/j.matchemphys.2017.09.051
Bai Y, Roncancio D, Suo Y, Shao Y, Zhang D, Zhou C (2019) A method based on amino-modified magnetic nanoparticles to extract DNA for PCR-based analysis. Colloids Surf Biointerfaces. https://doi.org/10.1016/j.colsurfb.2019.03.005
Bai Y, Cui Y, Paoli GC, Shi C, Wang D, Zhou M, Zhang L, Shi X (2016) Synthesis of amino-rich silica-coated magnetic nanoparticles for the efficient capture of DNA for PCR. Colloids Surf Biointerfaces. https://doi.org/10.1016/j.colsurfb.2016.05.003
Corem-Salkmon E, Ram Z, Daniels D, Perlstein B, Last D, Salomon S, Tamar G, Shneor R, Guez D, Margel S, Mardor Y (2011) Convection-enhanced delivery of methotrexate-loaded maghemite nanoparticles. Int J Nanomedicine 6:1595–1602. https://doi.org/10.2147/IJN.S23025
da Silva RJ, Maciel BG, Medina-Llamas JC, Chávez-Guajardo AE, Alcaraz-Espinoza JJ, Pinto de Melo C (2019) Extraction of plasmid DNA by use of a magnetic maghemite-polyaniline nanocomposite. Anal Biochem 575:27–35. https://doi.org/10.1016/j.ab.2019.03.013
Demangeat E, Pédrot M, Dia A, Bouhnik-Le-Coz M, Grasset F, Hanna K, Kamagate M, Cabello-Hurtado F (2018) Colloidal and chemical stabilities of iron oxide nanoparticles in aqueous solutions: The interplay of structural, chemical and environmental drivers. Environ Sci NANO 5:992–1001. https://doi.org/10.1039/c7en01159h
Diogo MM, Queiroz JA, Prazeres DMF (2005) Chromatography of plasmid DNA. J Chromatogr A 1069:3–22. https://doi.org/10.1016/j.chroma.2004.09.050
Gai L, Li Z, Hou Y, Jiang H, Han X, Ma W (2010) Preparation of core-shell Fe3O4/SiO2 microspheres as adsorbents for purification of DNA. J Phys D Appl Phys 43:254. https://doi.org/10.1088/0022-3727/43/44/445001
Ghaemi M, Absalan G (2014) Study on the adsorption of DNA on Fe3O4 nanoparticles and on ionic liquid-modified Fe3O4 nanoparticles. Microchim Acta. https://doi.org/10.1007/s00604-013-1040-5
Gómez Pérez A, González-Martínez E, Díaz Águila CR, González-Martínez DA, González Ruiz G, García Artalejo A, Yee-Madeira H (2020) Chitosan-coated magnetic iron oxide nanoparticles for DNA and rhEGF separation. Colloids Surf Phys Eng Asp 591:124500. https://doi.org/10.1016/j.colsurfa.2020.124500
Gupta AK, Gupta M (2005) Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications. Biomaterials 26:3995–4021. https://doi.org/10.1016/j.biomaterials.2004.10.012
Gutiérrez L, De La Cueva L, Moros M, Mazarío E, De Bernardo S, De La Fuente JM, Morales MP, Salas G (2020) Aggregation effects on the magnetic properties of iron oxide colloids. Nanotechnology 30:2025. https://doi.org/10.1088/1361-6528/aafbff
He X, Huo H, Wang K, Tan W, Gong P, Ge J (2007) Plasmid DNA isolation using amino-silica coated magnetic nanoparticles (ASMNPs). Talanta 73:764–769. https://doi.org/10.1016/j.talanta.2007.04.056
Hou Y, Han X, Chen J, Li Z, Chen X, Gai L (2013) Isolation of PCR-ready genomic DNA from Aspergillus niger cells with Fe3O4/SiO2 microspheres. Sep Purif Technol 116:5421. https://doi.org/10.1016/j.seppur.2013.05.033
Hu LL, Hu B, Shen LM, Zhang DD, Chen XW, Wang JH (2015) Polyethyleneimine-iron phosphate nanocomposite as a promising adsorbent for the isolation of DNA. Talanta 132:857–863. https://doi.org/10.1016/j.talanta.2014.10.047
Huang J, Wang L, Lin R, Wang AY, Yang L, Kuang M, Qian W, Mao H (2013) Casein-coated iron oxide nanoparticles for high MRI contrast enhancement and efficient cell targeting. ACS Appl Mater Interfaces 5:4632–4639. https://doi.org/10.1021/am400713j
Jain TK, Foy SP, Erokwu B, Dimitrijevic S, Flask CA, Labhasetwar V (2009) Magnetic resonance imaging of multifunctional pluronic stabilized iron-oxide nanoparticles in tumor-bearing mice. Biomaterials 30:6748–6756. https://doi.org/10.1016/j.biomaterials.2009.08.042
Je HH, Noh S, Hong SG, Ju Y, Kim J, Hwang DS (2017) Cellulose nanofibers for magnetically-separable and highly loaded enzyme immobilization. Chem Eng J 323:425–433. https://doi.org/10.1016/j.cej.2017.04.110
Jiang H, Han X, Li Z, Chen X, Hou Y, Gai L, Li D, Lu X, Fu T (2012) Superparamagnetic core-shell structured microspheres carrying carboxyl groups as adsorbents for purification of genomic DNA. Colloids Surf Phys Eng Asp 401:74–80. https://doi.org/10.1016/j.colsurfa.2012.03.024
Kajjumba GW, Yıldırım E, Aydın S, Emik S, Ağun T, Osra F, Wasswa J (2019) A facile polymerisation of magnetic coal to enhanced phosphate removal from solution. J Environ Manage 247:356–362. https://doi.org/10.1016/j.jenvman.2019.06.088
Kaşgöz H, Özgümüš S, Orbay M (2003) Modified polyacrylamide hydrogels and their application in removal of heavy metal ions. Polymer (guildf) 44:1785–1793. https://doi.org/10.1016/S0032-3861(03)00033-8
Khramtsov P, Barkina I, Kropaneva M, Bochkova M, Timganova V, Nechaev A, Byzov I, Zamorina S, Yermakov A, Rayev M (2019) Magnetic nanoclusters coated with albumin, casein, and gelatin: Size tuning, relaxivity, stability, protein corona, and application in nuclear magnetic resonance immunoassay. Nanomaterials. https://doi.org/10.3390/nano9091345
Korolev VV, Ramazanova AG, Blinov AV (2002) Adsorption of surfactants on superfine magnetite, 2002.
Li K, Lai Y, Zhang W, Jin L (2011) Fe2O3@Au core/shell nanoparticle-based electrochemical DNA biosensor for Escherichia coli detection. Talanta 84:607–613. https://doi.org/10.1016/j.talanta.2010.12.042
Mahdavi M, Bin Ahmad M, Haron MJ, Namvar F, Nadi B, Ab Rahman MZ, Amin J (2013) Synthesis, surface modification and characterisation of biocompatible magnetic iron oxide nanoparticles for biomedical applications. Molecules 18:7533–7548. https://doi.org/10.3390/molecules18077533
Maity D, Agrawal DC (2007) Synthesis of iron oxide nanoparticles under oxidizing environment and their stabilization in aqueous and non-aqueous media. J Magn Magn Mater 308:46–55. https://doi.org/10.1016/j.jmmm.2006.05.001
Massart R (1981) Preparation of aqueous magnetic liquids in alkaline and acidic media. IEEE Trans Magn 17:1247–1248. https://doi.org/10.1109/TMAG.1981.1061188
Min JH, Woo MK, Yoon HY, Jang JW, Wu JH, Lim CS, Kim YK (2014) Isolation of DNA using magnetic nanoparticles coated with dimercaptosuccinic acid. Anal Biochem 447:114–118. https://doi.org/10.1016/j.ab.2013.11.018
Mohammed L, Gomaa HG, Ragab D, Zhu J (2017) Magnetic nanoparticles for environmental and biomedical applications: a review. Particuology 30:1–14. https://doi.org/10.1016/j.partic.2016.06.001
Nakagawa T, Tanaka T, Niwa D, Osaka T, Takeyama H, Matsunaga T (2005) Fabrication of amino silane-coated microchip for DNA extraction from whole blood. J Biotechnol 116:105–111. https://doi.org/10.1016/j.jbiotec.2004.08.018
Namdeo M, Saxena S, Tankhiwale R, Bajpai M, Mohan YM, Bajpai SK (2008) Magnetic nanoparticles for drug delivery applications. J Nanosci Nanotechnol 8:3247–3271. https://doi.org/10.1166/jnn.2008.399
Perçin I, Karakoç V, Akgöl S, Aksöz E, Denizli A (2012) Poly(hydroxyethyl methacrylate) based magnetic nanoparticles for plasmid DNA purification from Escherichia coli lysate. Mater Sci Eng C 32:1133–1140. https://doi.org/10.1016/j.msec.2012.02.031
Pershina AG, Sazonov AE, Filimonov VD (2014) Magnetic nanoparticles–DNA interactions: design and applications of nanobiohybrid systems. Russ Chem Rev 83:299–322. https://doi.org/10.1070/rc2014v083n04abeh004412
Pourjavadi A, Kohestanian M, Streb C (2020) pH and thermal dual-responsive poly(NIPAM-co-GMA)-coated magnetic nanoparticles via surface-initiated RAFT polymerization for controlled drug delivery. Mater Sci Eng C 108:110418. https://doi.org/10.1016/j.msec.2019.110418
Rahman MM, Elaissari A (2011) Temperature and magnetic dual responsive microparticles for DNA separation. Sep Purif Technol. https://doi.org/10.1016/j.seppur.2011.07.030
Rittich B, Španová A (2013) SPE and purification of DNA using magnetic particles. J Sep Sci 36:2472–2485. https://doi.org/10.1002/jssc.201300331
Rittich B, Španová A, Horák D, Beneš MJ, Klesnilová L, Petrová K, Rybnikář A (2006) Isolation of microbial DNA by newly designed magnetic particles. Colloids Surfaces B Biointerfaces 52:143–148. https://doi.org/10.1016/j.colsurfb.2006.04.012
Shaterabadi Z, Nabiyouni G, Soleymani M (2017) High impact of in situ dextran coating on biocompatibility, stability and magnetic properties of iron oxide nanoparticles. Mater Sci Eng C 75:947–956. https://doi.org/10.1016/j.msec.2017.02.143
Sheng W, Wei W, Li J, Qi X, Zuo G, Chen Q, Pan X, Dong W (2016) Amine-functionalized magnetic mesoporous silica nanoparticles for DNA separation. Appl Surf Sci. https://doi.org/10.1016/j.apsusc.2016.07.061
Sosa-Acosta JR, Silva JA, Fernández-Izquierdo L, Díaz-Castañón S, Ortiz M, Zuaznabar-Gardona JC, Díaz-García AM (2018) Iron Oxide Nanoparticles (IONPs) with potential applications in plasmid DNA isolation. Colloids Surfaces Physicochem Eng Asp. https://doi.org/10.1016/j.colsurfa.2018.02.062
Sun H, Hong J, Meng F, Gong P, Yu J, Xue Y, Zhao S, Xu D, Dong L, Yao S (2006) Novel core-shell structure polyacrylamide-coated magnetic nanoparticles synthesized via photochemical polymerization. Surf Coatings Technol 201:250–254. https://doi.org/10.1016/j.surfcoat.2005.11.112
Tanaka T, Sakai R, Kobayashi R, Hatakeyama K, Matsunaga T (2009) Contributions of phosphate to DNA adsorption/desorption behaviors on aminosilane-modified magnetic nanoparticles contributions of phosphate to DNA adsorption/desorption behaviors on aminosilane-modified magnetic nanoparticles. Society 25:2956–2961
Tietze R, Zaloga J, Unterweger H, Lyer S, Friedrich RP, Janko C, Pöttler M, Dürr S, Alexiou C (2015) Magnetic nanoparticle-based drug delivery for cancer therapy. Biochem Biophys Res Commun 468:463–470. https://doi.org/10.1016/j.bbrc.2015.08.022
Trachtová Š, Španová A, Tóth J, Prettl Z, Horák D, Gyenis J, Rittich B (2015) Solid-phase DNA isolation from food matrices using hydrophilic magnetic microspheres. Food Bioprod Process 94:375–381. https://doi.org/10.1016/j.fbp.2014.05.001
Unsoy G, Khodadust R, Yalcin S, Mutlu P, Gunduz U (2014) Synthesis of Doxorubicin loaded magnetic chitosan nanoparticles for pH responsive targeted drug delivery. Eur J Pharm Sci 62:243–250. https://doi.org/10.1016/j.ejps.2014.05.021
Wei H, Bruns OT, Kaul MG, Hansen EC, Barch M, Wiśniowska A, Chen O, Chen Y, Li N, Okada S, Cordero JM, Heine M, Farrar CT, Montana DM, Adam G, Ittrich H, Jasanoff A, Nielsen P, Bawendi MG (2017) Exceedingly small iron oxide nanoparticles as positive MRI contrast agents. Proc Natl Acad Sci U S A 114:2325–2330. https://doi.org/10.1073/pnas.1620145114
Xu H, Aguilar ZP, Yang L, Kuang M, Duan H, Xiong Y, Wei H, Wang A (2011) Antibody conjugated magnetic iron oxide nanoparticles for cancer cell separation in fresh whole blood. Biomaterials 32:9758–9765. https://doi.org/10.1016/j.biomaterials.2011.08.076
Yang K, Peng H, Wen Y, Li N (2010) Re-examination of characteristic FTIR spectrum of secondary layer in bilayer oleic acid-coated Fe 3 O 4 nanoparticles. Appl Surf Sci. https://doi.org/10.1016/j.apsusc.2009.11.079
Yoshikawa K, Hirota S, Makita N, Yoshikawa Y (2010) Compaction of DNA induced by like-charge protein: opposite salt-effect against the polymer-salt-induced condensation with neutral polymer. J Phys Chem Lett 1:1763–1766. https://doi.org/10.1021/jz100569e
Yoza B, Matsumoto M, Matsunaga T (2002) DNA extraction using modified bacterial magnetic particles in the presence of amino silane compound. J Biotechnol 94:217–224. https://doi.org/10.1016/S0168-1656(01)00427-8
Acknowledgements
This work was supported financially by the Research Fund of Istanbul University, Turkey (Grant Number FBA-2017-23031.)
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Yıldırım, E., Arıkan, B., Yücel, O. et al. Synthesis and characterization of amino functional poly(acrylamide) coated Fe3O4 nanoparticles and investigation of their potential usage in DNA isolation. Chem. Pap. 76, 5747–5759 (2022). https://doi.org/10.1007/s11696-022-02293-y
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DOI: https://doi.org/10.1007/s11696-022-02293-y