Abstract
The monomeric hemeprotein myoglobin (Mb) has immense biological importance as it is first released from damaged muscles in case of an injury. Since excessive release of Mb damages kidney and causes acute renal failure, monitoring of Mb is relevant. A novel and cost-effective fluorescence sensor for label free determination of Mb has been developed. The turn-off assay is based on methionine stabilized palladium nanoclusters (Met-PdNCs). The Met-PdNCs have been characterized by spectroscopic techniques such as fluorescence, UV–Vis spectroscopy, FTIR and microscopic technique TEM along with DLS. The quenching mechanism was attributed to the synergistic effects of agglomeration-induced quenching and inner filter effect. The developed sensor exhibited a linear range of 1.00 × 10−5 M to 8.00 × 10−7 M with a limit of detection of 1.67 × 10−7 M. The practicality of the developed Mb sensor was validated through spike recovery analysis and a classic spectrophotometric method.
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The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
The authors Goldamol S Pallam and Manna Rachel Mathew thank University Grants Commission for research fellowship. The author Sanu K. Anand thanks Cochin University of Science and Technology for research fellowship. The authors would like to acknowledge Department of Science and Technology, Government of India, for financial assistance in the form of research project (File. No. DST/INT/POL/P-24/2016).
Funding
The authors Goldamol S Pallam and Manna Rachel Mathew thank University Grants Commission for research fellowship. The author Sanu K. Anand thanks Cochin University of Science and Technology for research fellowship. The authors would like to acknowledge Department of Science and Technology, Government of India, for financial assistance in the form of research project (File. No. DST/INT/POL/P-24/2016).
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Goldamol S Pallam, Sanu K. Anand, Manna Rachel Mathew and K. Girish Kumar contributed to conceptualization; Goldamol S Pallam was involved in methodology, formal analysis and investigation and writing—original draft preparation; Sanu K. Anand and Manna Rachel Mathew contributed to writing—review and editing; and K. Girish Kumar was involved in funding acquisition, resources and supervision.
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Pallam, G.S., Anand, S.K., Mathew, M.R. et al. Palladium nanocluster-based fluorescent sensing platform via synergistic effects of inner filter effect and agglomeration-induced quenching for myoglobin determination. Chem. Pap. 76, 4807–4815 (2022). https://doi.org/10.1007/s11696-022-02194-0
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DOI: https://doi.org/10.1007/s11696-022-02194-0