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Antioxidative, anticancer, and antibacterial activities of a nanoemulsion-based gel containing Myrtus communis L. essential oil

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Abstract

Myrtus communis L. is a common medicinal plant with a wide range of biological properties. In this study, an attempt was made to improve its essential oil’s antioxidant, anticancer, and antibacterial activities by preparing a nanogel dosage form. Alpha-pinene (29.7%), 1,8-cineole (25.8%), linalool (9.1%), linalool acetate (5.9%), and geranyl acetate (3.4%) were identified as five major compounds in the essential oil using GC–MS analysis. Optimum nanoemulsion with a droplet size of 179 ± 7 nm and a narrow droplet size distribution (SPAN 0.96) was gelified by the addition of carboxymethyl cellulose (3 w/v %). The rheometry analysis at shear rates of 0.1–100 1/s showed the viscosity was fully fitted with the Carreau-Yasuda model. The nanogel with IC50 132.6 µg/mL was 4 folds more potent than the bulk essential oil (IC50: 580.8 µg/mL) against A-375 melanoma cells. Besides, after treatment of Escherichia coli and Staphylococcus aureus with 1000 µg/mL of the nanogel and the bulk essential oil, their growths were observed at 37.5 and 59.1% as well as 21.4 and 40.6%. Besides, antioxidant activity was investigated using DPPH assay; the nanogel was significantly more potent (P < 0.001) than that of bulk essential oil at all examined concentrations (62.6–1000 µg/mL). Furthermore, polyethylene oxide-gelatin electrospun nanofibers (diameter of 359 ± 36 nm) with no effects on the cancer cell and the bacterial growth were proposed as lesion dressing after-treatment with the nanogel. Therefore, the stained nanofiber with the nanogel could be considered a natural potent anticancer and antibacterial agent in vivo study.

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Funding

Fasa University of Medical Sciences supported this study, Grant No. 400178.

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Authors and Affiliations

  1. Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran

    Ghazaal Roozitalab & Fatemeh Rasti

  2. Student Research Center Committee, Fasa University of Medical Sciences, Fasa, Iran

    Ghazaal Roozitalab & Fatemeh Rasti

  3. Research Center of Advanced Technologies in Medicine, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran

    Yaser Yousefpoor

  4. Khalil Abad Health Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Yaser Yousefpoor

  5. Department of Microbiology, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran

    Abbas Abdollahi

  6. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Mojdeh Safari

  7. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Fasa University of Medical Sciences, Fasa, Iran

    Mahmoud Osanloo

Authors
  1. Ghazaal Roozitalab
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  2. Yaser Yousefpoor
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  3. Abbas Abdollahi
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  4. Mojdeh Safari
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  5. Fatemeh Rasti
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  6. Mahmoud Osanloo
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Contributions

GhR prepared the nanogel and nanofibers and performed MTT and DPPH assays. GhR and YY wrote the MS. AA performed the antibacterial tests. MS interpreted ATR-FTIR. FR contributed to the preparation of nanogel and MTT assay. MO design of the study, statistical analysis, and revised the MS. All authors contributed to drafting MS and approved the final version.

Corresponding author

Correspondence to Mahmoud Osanloo.

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Researchers have no conflict of interest in this study.

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This study has been ethically approved by the Fasa University of Medical Sciences’ ethical committee, IR.FUMS.REC.1400.129.

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This research did not involve human study; thus, no constant form was used.

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Roozitalab, G., Yousefpoor, Y., Abdollahi, A. et al. Antioxidative, anticancer, and antibacterial activities of a nanoemulsion-based gel containing Myrtus communis L. essential oil. Chem. Pap. 76, 4261–4271 (2022). https://doi.org/10.1007/s11696-022-02185-1

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