Abstract
A sensitive and straightforward magnetic dispersive micro-solid-phase extraction as a sample preparation procedure coupled with high-performance liquid chromatography was developed to preconcentrate and clean up four antibiotics, including azithromycin (AZI), doxycycline (DOX), amoxicillin (AMO), and tetracycline (TC), from biological samples. For this purpose, several nanofluids with a magnetic colloidal gel structure were prepared based on the dispersion of three synthetic nanoparticles in dimethylformamide in the presence of an ionic liquid individually. The nanofluid preparation leads to a reduction in the extraction and desorption time and sorbent consumption through an increase in the sorbent surface area. Usage of the nanofluid can also increase the sorbent dispersion efficiency without using a dispersion agent. The ability of the magnetic colloidal gels to simultaneously extract the antibiotics was compared together. An experimental design including screening and optimization steps was utilized to optimize effective factors in the micro-extraction method. Under the optimum condition, the proposed method has linearity in the concentration ranges of 0.01–12.0, 0.01–12.0, 0.01–15.0, 0.05–15.0 ng mL−1 with a determination coefficient (R2) of 0.9940, 0.9951, 0.9939, and 0.9946, for the determination of AZI, DOX, AMO, and TC in water samples, respectively. LODs and LOQs were in the range of 0.003–0.015 and 0.01–0.05 ng mL−1 to determine the analytes, respectively. Intra-day RSDs of the method (n = 5) for the determination of AZI, DOX, AMO, and TC with a concentration of 1.0 ng mL−1 in water samples were 3.63, 3.49, 3.91, and 3.50%, respectively. Analysis of real samples such as urine and hair samples indicated that the proposed method has proper recoveries in the range of 86.0–101.4% with a relative standard deviation in the range of 3.76–5.36%.
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References
Aga DS, Lenczewski M, Snow D, Muurinen J, Sallach JB, Wallace JS (2016) Challenges in the measurement of antibiotics and in evaluating their impacts in agroecosystems: a critical review. J Environ Qual 45:407–419. https://doi.org/10.2134/jeq2015.07.0393
Andrade-Eiroa A, Canle M, Leroy-Cancellieri V, Cerdà V (2016) Solid-phase extraction of organic compounds: a critical review (Part I). TrAC Trends Anal Chem 80:641–654. https://doi.org/10.1016/j.trac.2015.08.015
Asadi A, Aberoumand S, Moradikazerouni A, Pourfattah F, Żyła G, Estellé P, Mahian O, Wongwises S, Nguyen HM, Arabkoohsar A (2019) Recent advances in preparation methods and thermophysical properties of oil-based nanofluids: a state-of-the-art review. Powder Technol 352:209–226. https://doi.org/10.1016/j.powtec.2019.04.054
Ayazi Z (2017) Application of nanocomposite-based sorbents in microextraction techniques: a review. Analyst 142:721–739. https://doi.org/10.1039/C6AN02744J
Azzouz A, Kailasa SK, Lee SS, Rascón AJ, Ballesteros E, Zhang M, Kim K-H (2018) Review of nanomaterials as sorbents in solid-phase extraction for environmental samples. TrAC Trends Anal Chem 108:347–369. https://doi.org/10.1016/j.trac.2018.08.009
Bitew Z, Amare M (2020) Recent reports on electrochemical determination of selected antibiotics in pharmaceutical formulations: a mini review. Electrochem Commun 121:106863. https://doi.org/10.1016/j.elecom.2020.106863
Chen Z, Yu G, Wang Q (2020) Effects of climate and forest age on the ecosystem carbon exchange of afforestation. J Forest Res 31:365–374. https://doi.org/10.1007/s11676-019-00946-5
Cherkashina K, Vakh C, Lebedinets S, Pochivalov A, Moskvin L, Lezov A, Bulatov A (2018) An automated salting-out assisted liquid-liquid microextraction approach using 1-octylamine: On-line separation of tetracycline in urine samples followed by HPLC-UV determination. Talanta 184:122–127. https://doi.org/10.1016/j.talanta.2018.02.112
Devendiran DK, Amirtham VA (2016) A review on preparation, characterization, properties and applications of nanofluids. Renew Sustain Energy Rev 60:21–40. https://doi.org/10.1016/j.rser.2016.01.055
Ebrahimzadeh H, Yamini Y, Ara KM, Kamarei F, Khalighi-Sigaroodi F (2010) Determination of azithromycin in biological samples by LLLME combined with LC. Chromatographia 72:731–735. https://doi.org/10.1365/s10337-010-1692-9
Farouk F, Azzazy HM, Niessen WM (2015) Challenges in the determination of aminoglycoside antibiotics, a review. Anal Chim Acta 890:21–43. https://doi.org/10.1016/j.aca.2015.06.038
Ghorbani M, Chamsaz M, Rounaghi GH, Aghamohammadhasani M, Seyedin O, Lahoori NA (2016) Development of a novel ultrasonic-assisted magnetic dispersive solid-phase microextraction method coupled with high performance liquid chromatography for determination of mirtazapine and its metabolites in human urine and water samples employing experimental design. Anal Bioanalytical Chem 408:7719–7729. https://doi.org/10.1007/s00216-016-9869-1
Ghorbani M, Akbarzade S, Aghamohammadhasan M, Seyedin O, Lahoori NA (2018) Pre-concentration and determination of cadmium and lead ions in real water, soil and food samples using a simple and sensitive green solvent-based ultrasonic assisted dispersive liquid–liquid microextraction and graphite furnace atomic absorption spectrometry. Anal Methods 10:2041–2047. https://doi.org/10.1039/C8AY00398J
Ghorbani M, Aghamohammadhasan M, Shams A, Tajfirooz F, Pourhassan R, Khosravi SRB, Karimi E, Jampour A (2019a) Ultrasonic assisted magnetic dispersive solid phase microextraction for preconcentration of two nonsteroidal anti-inflammatory drugs in real water, biological and milk samples employing an experimental design. Microchem J 145:1026–1035. https://doi.org/10.1016/j.microc.2018.12.019
Ghorbani M, Aghamohammadhassan M, Chamsaz M, Akhlaghi H, Pedramrad T (2019b) Dispersive solid phase microextraction. TrAC Trends Anal Chem 118:793–809. https://doi.org/10.1016/j.trac.2019.07.012
Ghorbani M, Pedramrad T, Aghamohammadhasan M, Seyedin O, Akhlaghi H, Lahoori NA (2019c) Simultaneous clean-up and determination of Cu (II), Pb (II) and Cr (III) in real water and food samples using a magnetic dispersive solid phase microextraction and differential pulse voltammetry with a green and novel modified glassy carbon electrode. Microchem J 147:545–554. https://doi.org/10.1016/j.microc.2019.03.072
Ghorbani M, Aghamohammadhassan M, Ghorbani H, Zabihi A (2020) Trends in sorbent development for dispersive micro-solid phase extraction. Microchem J 158:105250. https://doi.org/10.1016/j.microc.2020.105250
Ghorbani M, Mohammadi P, Keshavarzi M, Saghi MH, Mohammadi M, Shams A, Aghamohammadhasan M (2021) Simultaneous determination of organophosphorus pesticides residues in vegetable, fruit juice, and milk samples with magnetic dispersive micro solid-phase extraction and chromatographic method; recruitment of simplex lattice mixture design for optimization of novel sorbent composites. Anal Chim Acta 1178:338802. https://doi.org/10.1016/j.aca.2021.338802
Gissawong N, Boonchiangma S, Mukdasai S, Srijaranai S (2019) Vesicular supramolecular solvent-based microextraction followed by high performance liquid chromatographic analysis of tetracyclines. Talanta 200:203–211. https://doi.org/10.1016/j.talanta.2019.03.049
Gordi Z, Ghorbani M, Ahmadian Khakhiyani M (2020) Adsorptive removal of enrofloxacin with magnetic functionalized graphene oxide@ Metal-organic frameworks employing D-optimal mixture design. Water Environ Res 92:1935–1947. https://doi.org/10.1002/wer.1346
Hamidi S, Azami A, Aghdam EM (2019) A novel mixed hemimicelles dispersive micro-solid phase extraction using ionic liquid functionalized magnetic graphene oxide/polypyrrole for extraction and preconcentration of methotrexate from urine samples followed by the spectrophotometric method. Clin Chim Acta 488:179–188. https://doi.org/10.1016/j.cca.2018.11.006
Jha RR, Singh N, Kumari R, Patel DK (2017) Ultrasound-assisted emulsification microextraction based on a solidified floating organic droplet for the rapid determination of 19 antibiotics as environmental pollutants in hospital drainage and Gomti river water. J Separation Sci 40:2694–2702. https://doi.org/10.1002/jssc.201700170
Jia S, Li J, Park S-R, Ryu Y, Park IH, Park JH, Hong S-S, Kwon SW, Lee J (2013) Combined application of dispersive liquid–liquid microextraction based on the solidification of floating organic droplets and charged aerosol detection for the simple and sensitive quantification of macrolide antibiotics in human urine. J Pharmaceutical Biomed Analysis 86:204–213. https://doi.org/10.1016/j.jpba.2013.07.024
Khoshnood RS, Khoshnoud DS (2019) Structural, magnetic, and photocatalytic properties in Bi 0.83− x La 0.17 Y x FeO 3 nanoparticles. Appl Phys A 125:750. https://doi.org/10.1007/s00339-019-3040-8
Kim C, Ryu H-D, Chung EG, Kim Y, Lee J-K (2018) A review of analytical procedures for the simultaneous determination of medically important veterinary antibiotics in environmental water: sample preparation, liquid chromatography, and mass spectrometry. J Environ Manage 217:629–645. https://doi.org/10.1016/j.jenvman.2018.04.006
Kumar SB, Arnipalli SR, Ziouzenkova O (2020) Antibiotics in food chain: the consequences for antibiotic resistance. Antibiotics 9:688. https://doi.org/10.3390/antibiotics9100688
Li S, Lin Y-H, Zhang B-P, Wang Y, Nan C-W (2010) Controlled fabrication of BiFeO3 uniform microcrystals and their magnetic and photocatalytic behaviors. J Phys Chem C 114:2903–2908. https://doi.org/10.1021/jp910401u
Minea AA, Murshed SS (2018) A review on development of ionic liquid based nanofluids and their heat transfer behavior. Renew Sustain Energy Rev 91:584–599. https://doi.org/10.1016/j.rser.2018.04.021
Mohammadi P, Ghorbani M, Mohammadi P, Keshavarzi M, Rastegar A, Aghamohammadhassan M, Saghafi A (2021) Dispersive micro solid-phase extraction with gas chromatography for determination of Diazinon and Ethion residues in biological, vegetables and cereal grain samples, employing D-optimal mixture design. Microchem J 160:105680. https://doi.org/10.1016/j.microc.2020.105680
Mookantsa S, Dube S, Nindi M (2016) Development and application of a dispersive liquid–liquid microextraction method for the determination of tetracyclines in beef by liquid chromatography mass spectrometry. Talanta 148:321–328. https://doi.org/10.1016/j.talanta.2015.11.006
Omidi F, Behbahani M, Khadem M, Golbabaei F, Shahtaheri SJ (2019) Application of a new sample preparation method based on surfactant-assisted dispersive micro solid phase extraction coupled with ultrasonic power for easy and fast simultaneous preconcentration of toluene and xylene biomarkers from human urine samples. J Iranian Chem Soc 16:1131–1138. https://doi.org/10.1007/s13738-018-01588-w
Oniciuc E-A, Likotrafiti E, Alvarez-Molina A, Prieto M, López M, Alvarez-Ordóñez A (2019) Food processing as a risk factor for antimicrobial resistance spread along the food chain. Curr Opin Food Sci 30:21–26. https://doi.org/10.1016/j.cofs.2018.09.002
Rezaeifar Z, Es’ haghi, Z, Rounaghi GH, Chamsaz M (2016) Hyperbranched polyglycerol/graphene oxide nanocomposite reinforced hollow fiber solid/liquid phase microextraction for measurement of ibuprofen and naproxen in hair and waste water samples. J Chromatogr B 1029:81–87. https://doi.org/10.1016/j.jchromb.2016.07.010
Sarafraz-Yazdi A, Amiri A (2010) Liquid-phase microextraction. TrAC Trends. Anal Chem 29:1–14. https://doi.org/10.1016/j.trac.2009.10.003
Shirani M, Akbari-Adergani B, Nodeh HR, Shahabuddin S (2020) Ultrasonication-facilitated synthesis of functionalized graphene oxide for ultrasound-assisted magnetic dispersive solid-phase extraction of amoxicillin, ampicillin, and penicillin G. Microchim Acta 187:1–11. https://doi.org/10.1007/s00604-020-04605-z
Spurling GK, Del Mar CB, Dooley L, Foxlee R, Farley R (2013) Delayed antibiotics for respiratory infections. Cochrane Database of Systematic Reviews.
Xie WY, Shen Q, Zhao F (2018) Antibiotics and antibiotic resistance from animal manures to soil: a review. Euro J Soil Sci 69:181–195. https://doi.org/10.1111/ejss.12494
Yang Y, Song W, Lin H, Wang W, Du L, Xing W (2018) Antibiotics and antibiotic resistance genes in global lakes: a review and meta-analysis. Environ Int 116:60–73. https://doi.org/10.1016/j.envint.2018.04.011
Yousefi SM, Shemirani F, Ghorbanian SA (2017) Deep eutectic solvent magnetic bucky gels in developing dispersive solid phase extraction: application for ultra trace analysis of organochlorine pesticides by GC-micro ECD using a large-volume injection technique. Talanta 168:73–81. https://doi.org/10.1016/j.talanta.2017.03.020
Zabawa TP, Pucci MJ, Parr TR Jr, Lister T (2016) Treatment of Gram-negative bacterial infections by potentiation of antibiotics. Curr Opin Microbiol 33:7–12. https://doi.org/10.1016/j.mib.2016.05.005
Acknowledgements
The Azad University of Mashhad is appreciated for providing the laboratory space and also thank Dr. Parisa Mohammadi, Dr. Majid Keshavarzi, and Dr. Amir Esmailzadeh for their scientific support.
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Saghafi, A., Sanavi Khoshnood, R., Sanavi Khoshnoud, D. et al. Preconcentration and determination of four antibiotics in biological samples using nanofluid-assisted magnetic dispersive micro-solid-phase extraction coupled with high-performance liquid chromatography. Chem. Pap. 76, 901–911 (2022). https://doi.org/10.1007/s11696-021-01903-5
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DOI: https://doi.org/10.1007/s11696-021-01903-5