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Evaluation of the volatile from Lonicera macranthoides obtained with different processing methods by headspace–solid-phase microextraction–gas chromatography–tandem mass spectrometry (HS–SPME–GC–MS)

Chemical Papers volume 75, pages 3847–3857 (2021)Cite this article

Abstract

Headspace–solid-phase microextraction coupled to gas chromatography–tandem mass spectrometry (HS–SPME–GC–MS) method was used to determine the composition and content of volatiles in dried flower buds of Lonicera macranthoides obtained by five processing methods (PMs). Sixty-five compounds were identified, which included alcohols, aldehydes, ketones, hydrocarbons, acids, esters, terpenoids, and others. Alcohols were the most abundant compounds in most of the samples while acids were present in negligible amounts. Samples obtained by sun drying (PM4) and by heating in an oven with a programmed temperature cycle (PM2) were characterized by higher amounts of esters and terpenoids. PM4 resulted in larger numbers of compounds albeit in lower amounts. The principal component analysis confirmed that terpenoids and esters were the most prominent volatiles from PM2. The HS–SPME–GC–MS method can be used to rapidly ascertain the volatile content in L. macranthoides samples; the results herein provided provide a valuable source for comparing the effects of applying different processing methods.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 31370360&81703845) and the National Administration of Traditional Chinese Medicine (JDZX2015275).

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Authors and Affiliations

  1. Department of Pathology, Wangjing Hospital, China Academy of Chinese Medical Sciences, Beijing, 100102, People’s Republic of China

    Jingru Zhang, Yuan Wang, Chen Yang, Qiuyue Li, Jianqi Bai & Ping Zhang

  2. School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 100029, People’s Republic of China

    Jingru Zhang & Weidong Li

  3. Beijing Aerospace Fudao High-Tech.Co.Ltd., Beijing, 100854, People’s Republic of China

    Chunxiang Yang

  4. Wangjing Hospital, China Academy of Chinese Medical Sciences, Beijing, 100102, People’s Republic of China

    Liguo Zhu

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  1. Jingru Zhang
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  2. Chunxiang Yang
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  3. Liguo Zhu
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  4. Yuan Wang
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  5. Chen Yang
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  6. Qiuyue Li
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  8. Weidong Li
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  9. Ping Zhang
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Contributions

W.L. and J.Z. conceived and designed of the work. Y.W., C.Y. (Chen Yang) and J.B. cooperative on the experiment. J.Z. and C.Y. analyzed or interpreted of data. J. Z. wrote the manuscript. P.Z. and W.L. ensured funding for project contributed materials, and they are the corresponding author. L.Z., Q.L and P.Z. helped to revise the manuscript. All authors have read and approved the manuscript.

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Correspondence to Weidong Li or Ping Zhang.

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Zhang, J., Yang, C., Zhu, L. et al. Evaluation of the volatile from Lonicera macranthoides obtained with different processing methods by headspace–solid-phase microextraction–gas chromatography–tandem mass spectrometry (HS–SPME–GC–MS). Chem. Pap. 75, 3847–3857 (2021). https://doi.org/10.1007/s11696-021-01560-8

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  • DOI: https://doi.org/10.1007/s11696-021-01560-8

Keywords

  • Lonicerae flos
  • HS–SPME–GC–MS
  • Volatiles
  • Processing methods
  • PCA