Abstract
Vegetable oils present great economic importance as they can be used and marketed by different industries based on their properties. Currently, several techniques can be employed to extract oils from seeds. However, novel techniques are not easily applicable in any environment. Thus, this study aimed to evaluate the efficiency of simple techniques; Bligh and Dyer, Soxhlet, and acid hydrolysis, in extracting oil from canola, flaxseed, soybean, and sunflower seeds. The sum of fatty acids found in extracted oils indicated Bligh and Dyer as the most efficient method among those assessed. Furthermore, the lower content of polyunsaturated fatty acids quantified in oils obtained by Soxhlet or acid hydrolysis may result from oxidation triggered using high temperatures required by both methods.
Graphic abstract
Different lipid extraction methodologies were compared for the determination of fatty acid content in seeds.
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References
Akkaya MR (2018) Fatty acid compositions of sunflowers (Helianthus annuus L.) grown in east Mediterranean region. Rivista Italiana Delle Sostanze Grasse 95(4):239–247
Alves AQ, da Silva Jr VA, Góes AJS, Silva MS et al (2019) The fatty acid composition of vegetable oils and their potential use in wound care. Adv Skin Wound Care 32(8):1–8. https://doi.org/10.1097/01.ASW.0000557832.86268.64
AOAC (1995) Association of official analytical chemists international. Official methods of analysis, 16th edn. AOAC, Arlington
Biondo PB, dos Santos VJ, Montanher PF, de OS Junior O, Matsushita M, Almeida VC, Visentainer JV (2015) A new method for lipid extraction using low-toxicity solvents developed for canola (Brassica napus L.) and soybean (Glycine max L. Merrill) seeds. Anal Methods 7(23):9773–9778. https://doi.org/10.1039/C5AY02243F
Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol A 37:911–917
Brum AAS, Arruda LFD (2009) métodos de extração e qualidade da fração lipídica de matérias-primas de origem vegetal e animal. Química Nova 32(4):849–854. https://doi.org/10.1590/s0100-40422009000400005
Codex Alimentarius (2019) Standard for Named Vegetable Oils, Codex Stan 210–1999; Food and Agriculture Organization of the United States (FAO), World Health Organization (WHO), p 1–15. https://www.fao.org/fao-who-codexalimentarius/codex-texts/list-standards/en/. Accessed 30 Mar 2020
De Souza AT et al (2008) Supercritical extraction process and phase equilibrium of Candeia (Eremanthus erythropappus) oil using supercritical carbon dioxide. J Supercritical Fluids 47(2):182–187. https://doi.org/10.1016/j.supflu.2008.08.001
Galão OF, Carrão-Panizzi MC, Mandarino JMG, Júnior OOS et al (2014) Differences of fatty acid composition in Brazilian genetic and conventional soybeans (Glycine max (L.) Merrill) grown in different regions. Food Res Int 62:589–594. https://doi.org/10.1016/j.foodres.2014.04.025
Guidoni M, de Christo Scherer MM, Figueira MM, Schmitt EFP et al (2019) Fatty acid composition of vegetable oil blend and in vitro effects of pharmacotherapeutical skin care applications. Braz J Med Biol Res. https://doi.org/10.1590/1414-431x20188209
Hartman L, Lago RC (1973) Rapid preparation of fatty acid methyl esters from lipids. Lab Pract 22(6):475–476
Indelicato S, Bongiorno D, Pitonzo R, Di Stefano V, Calabrese V, Indelicato S, Avellone G (2017) Triacylglycerols in edible oils: determination, characterization, quantitation, chemometric approach and evaluation of adulterations. J Chromatogr A 1515:1–16. https://doi.org/10.1016/j.chroma.2017.08.002
Iverson SJ, Lang SL, Cooper MH (2001) Comparison of the Bligh and Dyer and Folch methods for total lipid determination in a broad range of marine tissue. Lipids 36(11):1283–1287. https://doi.org/10.1007/s11745-001-0843-0
Koubaa M, Mhemdi H, Barba FJ, Roohinejad S, Greiner R, Vorobiev E (2016) Oilseed treatment by ultrasounds and microwaves to improve oil yield and quality: an overview. Food Res Int 85:59–66. https://doi.org/10.1016/j.foodres.2016.04.007
Li P, Sakuragi K, Makino H (2019) Extraction techniques in sustainable biofuel production: a concise review. Fuel Process Technol 193:295–303. https://doi.org/10.1016/j.fuproc.2019.05.009
Mansour EA, El-Enin SA, Hamouda AS, Mahmoud HM (2019) Efficacy of extraction techniques and solvent polarity on lipid recovery from domestic wastewater microalgae. Environ Nanotechnol Monit Manag 12:100271. https://doi.org/10.1016/j.enmm.2019.100271
Maia EL, Rodriguez-Amaya DB (1993) Avaliação de um método simples e econômico para a metilação de ácidos graxos com lipídios de diversas espécies de peixes. Revista do Instituto Adolfo Lutz 53(1/2):27–35
Mejri J, Zarrouk Y, Hammami M (2018) Effect of torrefaction on the lipidic profile of sunflower seed oil. Rivista Italiana Delle Sostanze Grasse 95(3):161–166
Okolie CL, Akanbi TO, Mason B, Udenigwe CC, Aryee AN (2019) Influence of conventional and recent extraction technologies on physicochemical properties of bioactive macromolecules from natural sources: a review. Food Res Int 116:827–839. https://doi.org/10.1016/j.foodres.2018.09.018
Pederssetti MM, Palú F, Da Silva EA, Rohling JH, Cardozo-Filho L, Dariva C (2011) Extraction of canola seed (Brassica napus) oil using compressed propane and supercritical carbon dioxide. J Food Eng 102(2):189–196. https://doi.org/10.1016/j.jfoodeng.2010.08.018
Pereira MG et al (2017) Assessment of subcritical propane, ultrasound-assisted and Soxhlet extraction of oil from sweet passion fruit (Passiflora alata Curtis) seeds. J Supercrit Fluids 128:338–348. https://doi.org/10.1016/j.supflu.2017.03.021
Pradhan RC, Meda V, Rout PK, Naik S, Dalai AK (2010) Supercritical CO2 extraction of fatty oil from flaxseed and comparison with screw press expression and solvent extraction processes. J Food Eng 98(4):393–397. https://doi.org/10.1016/j.jfoodeng.2009.11.021
Rai A, Mohanty B, Bhargava R (2016) Supercritical extraction of sunflower oil: a central composite design for extraction variables. Food Chem 192:647–659. https://doi.org/10.1016/j.foodchem.2015.07.070
Ranjan A, Patil C, Moholkar VS (2010) Mechanistic assessment of microalgal lipid extraction. Ind Eng Chem Res 49(6):2979–2985. https://doi.org/10.1021/ie9016557
Ribeiro PPC, Dantas MM, Ribeiro KDDS, Dimenstein R, Damasceno KSFDS (2019) Determination of tocopherols and physicochemical properties of faveleira (Cnidoscolus quercifolius) seed oil extracted using different methods. Food Sci Technol (AHEAD). https://doi.org/10.1590/fst.24017
Sahena F, Zaidul ISM, Jinap S, Karim AA, Abbas KA, Norulaini NAN, Omar AKM (2009) Application of supercritical CO2 in lipid extraction—a review. J Food Eng 95(2):240–253. https://doi.org/10.1016/j.jfoodeng.2009.06.026
Savva SC, Kafatos A (2016) Vegetable oils: Dietary importance. https://doi.org/10.1016/B978-0-12-384947-2.00709-1
Senrayan J, Venkatachalam S (2019) A short extraction time of vegetable oil from Carica papaya L. seeds using continuous ultrasound acoustic cavitation: analysis of fatty acid profile and thermal behavior. J Food Process Eng 42(1):e12950. https://doi.org/10.1111/jfpe.12950
Shin EC, Hwang CE, Lee BW, Kim HT, Ko JM, Baek IY et al (2012) Chemometric approach to fatty acid profiles in soybean cultivars by principal component analysis (PCA). Prevent Nutr Food Sci 17(3):184. https://doi.org/10.3746/pnf.2012.17.3.184
Soxhlet F (1879) Die gewichtsanalytische bestimmung des milchfettes. Polytechnisches J 232:461–465
Tanamati AAC, Tanamati A, Ganzaroli JF, Sanchez JL, da Silva MV (2011) Comparative study of total lipids extraction methods in vegetable and animal samples. Rev Bras de Pesquisa em Alimentos 1(2):73–77
Ullah SR et al (2011) Fat extraction from acid-and base-hydrolyzed food samples using accelerated solvent extraction. J Agric Food Chem 59(6):2169–2174. https://doi.org/10.1021/jf104001d
Unglert M, Bockey D, Bofinger C, Buchholz B et al (2020) Action areas and the need for research in biofuels. Fuel 268:117227. https://doi.org/10.1016/j.fuel.2020.117227
Zanqui AB, de Morais DR, da Silva CM, Santos JM et al (2015) Subcritical extraction of flaxseed oil with n-propane: composition and purity. Food Chem 188:452–458. https://doi.org/10.1016/j.foodchem.2015.05.033
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The authors are grateful to CNPq, CAPES, and fundação araucária for providing financial backing.
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Financial support was provided to the authors by CAPES, CNPq and Fundação Araucária.
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OOS conceived and directed the project. JVV granted the equipment, reagents, and laboratory to carry out the experiments. PDSS, AdFBP, and RLGP performed the experiments. PDSS, AdFBP, RLGP, and VHMdC contributed to the results’ interpretation. CSFF did the results’ statistical analysis. VHMdC took the lead in writing the manuscript. AdFBP, PDSS, and RLGP revised the manuscript. RdS and VHMdC translated the article.
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da Cruz, V.H.M., Priori, R.L.G., Santos, P.D.S. et al. Evaluation of different conventional lipid extraction techniques’ efficiency in obtaining oil from oleaginous seeds. Chem. Pap. 75, 515–522 (2021). https://doi.org/10.1007/s11696-020-01324-w
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DOI: https://doi.org/10.1007/s11696-020-01324-w