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Evaluation of different conventional lipid extraction techniques’ efficiency in obtaining oil from oleaginous seeds

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Vegetable oils present great economic importance as they can be used and marketed by different industries based on their properties. Currently, several techniques can be employed to extract oils from seeds. However, novel techniques are not easily applicable in any environment. Thus, this study aimed to evaluate the efficiency of simple techniques; Bligh and Dyer, Soxhlet, and acid hydrolysis, in extracting oil from canola, flaxseed, soybean, and sunflower seeds. The sum of fatty acids found in extracted oils indicated Bligh and Dyer as the most efficient method among those assessed. Furthermore, the lower content of polyunsaturated fatty acids quantified in oils obtained by Soxhlet or acid hydrolysis may result from oxidation triggered using high temperatures required by both methods.

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Different lipid extraction methodologies were compared for the determination of fatty acid content in seeds.

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The data supporting the conclusions of this study are available within the article.


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The authors are grateful to CNPq, CAPES, and fundação araucária for providing financial backing.


Financial support was provided to the authors by CAPES, CNPq and Fundação Araucária.

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OOS conceived and directed the project. JVV granted the equipment, reagents, and laboratory to carry out the experiments. PDSS, AdFBP, and RLGP performed the experiments. PDSS, AdFBP, RLGP, and VHMdC contributed to the results’ interpretation. CSFF did the results’ statistical analysis. VHMdC took the lead in writing the manuscript. AdFBP, PDSS, and RLGP revised the manuscript. RdS and VHMdC translated the article.

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Correspondence to Oscar O. Santos.

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da Cruz, V.H.M., Priori, R.L.G., Santos, P.D.S. et al. Evaluation of different conventional lipid extraction techniques’ efficiency in obtaining oil from oleaginous seeds. Chem. Pap. 75, 515–522 (2021).

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