Skip to main content


Log in

Regenerated silk fibroin-based dressing modified with carnosine-bentonite nanosheets accelerates healing of second-degree burn wound

  • Original Paper
  • Published:
Chemical Papers Aims and scope Submit manuscript


Regenerated silk fibroin (RSF) is a promising candidate for skin repair due to its wound healing properties. RSF films have some drawbacks such as weak mechanical property, low porosity and poor absorption which limits their application as an ideal wound healing dressing. In this study, a novel RSF-based nanohybrid bearing carnosine (Car) intercalated bentonite (CarBen) nanosheets was prepared using solvent casting method and was applied in a second-degree burn wound model. RSF bearing different amounts of CarBen nanosheets (0, 1, 3 and 5 wt%) was prepared and fully characterized. RSF having 5 wt% of the modified Ben (RSF-CarBen5) showed superior mechanical property (ultimate stress of 8.36 ± 01 MPa and ultimate strain of 1501.33 ± 1.24%), and higher absorption capacity (1653.57 ± 0.05%) with favorable porosity (80.27%). RSF-CarBen5 was employed as dressing for healing of second-degree burn wound in rats. Histological evaluation showed considerable inflammation reduction in wound sites compared to the control groups. The wound treated with the nanohybrid showed significant epithelialization and angiogenesis on day 14 post-treatment, compared to the other groups (P < 0.05). The results showed that RSF bearing CarBen nanosheets have suitable therapeutic potential and can heal burn wounds appropriately.

Graphic abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others


Download references


This study was supported by a grant from the Research Council of Tehran University of Medical Sciences.

Author information

Authors and Affiliations


Corresponding authors

Correspondence to Ali Ramazani or Mehdi Khoobi.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 1826 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Azimzadeh Asiabi, P., Ramazani, A., Khoobi, M. et al. Regenerated silk fibroin-based dressing modified with carnosine-bentonite nanosheets accelerates healing of second-degree burn wound. Chem. Pap. 74, 3243–3257 (2020).

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: