2-Phenylethanol biooxidation by Gluconobacter oxydans: influence of cultivation conditions on biomass production and biocatalytic activity of cells
- 44 Downloads
Gluconobacter oxydans can be used as a whole-cell biocatalyst in many industrial processes focused on production of carbonyl and carboxylic compounds by oxidation of respective alcohols. However, high biocatalyst production costs limit its widespread industrial application. Therefore, the influence of Gluconobacter oxydans cultivation conditions on cell growth and the activity in biooxidation of 2-phenylethanol to phenylacetaldehyde and phenylacetic acid was investigated using batch and fed-batch cultivation cultures. The maximum total activity (given by the product of specific activity of cells and biomass concentration) was obtained by batch cultivation in a medium containing 25 g/L glycerol, at pH 4.5 and 20% oxygen saturation. In a fed-batch culture the best biomass growth was obtained with the feeding medium containing 125 g/L glycerol at a flow rate of 0.02 L/h per 0.5 L of starting culture volume. Although the final biomass concentration was around two times higher, the specific biotransformation activity of cells was only around 20% compared to the biocatalyst prepared by batch culture.
KeywordsGluconobacter oxydans Cultivation conditions 2-Phenylethanol oxidation Phenylacetic acid Phenylacetaldehyde
This article was created with the support of the Ministry of Education, Science, Research and Sport of the Slovak Republic within the Research and Development Operational Programme for the project ‘University Science Park of STU Bratislava’, ITMS 26240220084.
- Flickinger MC, Perlman D (1977) Application of oxygen enriched aeration in the conversion of glycerol to dihydroxyacetone by Gluconobacter melanogenus IFO 3293. Appl Environ Microbiol 33:706–712Google Scholar
- Gupta A, Singh VK, Qazi GN, Kumar A (2001) Gluconobacter oxydans: its biotechnological applications. J Mol Microbiol Biotechnol 3:445–456Google Scholar
- Hu Z-C, Tian S-Y, Ruan L-J, Zheng Y-G (2017) Repeated biotransformation of glycerol to 1,3-dihydroxyacetone by immobilized cells of Gluconobacter oxydans with glycerol- and urea-feeding strategy in a bubble column bioreactor. Bioresour Technol 233:144–149. https://doi.org/10.1016/j.biortech.2017.02.096 CrossRefGoogle Scholar
- Luttik MAH, Van Spanning R, Schipper D, Van Dijken JP, Pronk JT (1997) The low biomass yields of the acetic acid bacterium Acetobacter pasteurianus are due to a low stoichiometry of respiration-coupled proton translocation. Appl Environ Microbiol 63:3345–3351Google Scholar
- Navrátil M, Tkáč J, Švitel J, Danielsson B, Šturdík E (2001) Monitoring of the bioconversion of glycerol to dihydroxyacetone with immobilized Gluconobacter oxydans cell using thermometric flow injection analysis. Process Biochem 36:1045–1052. https://doi.org/10.1016/S0032-9592(00)00298-3 CrossRefGoogle Scholar
- Rabenhorst J, Gatfield I-L, Hilmer J-M (2003) Fermentative process for obtaining natural aromatic, aliphatic and thiocarboxylic acids and microorganism therefor. United States PatentGoogle Scholar
- Wei LJ, Zhou JL, Zhu DN, Cai BY, Lin JP, Hua Q, Wei DZ (2012) Functions of membrane-bound alcohol dehydrogenase and aldehyde dehydrogenase in the bio-oxidation of alcohols in Gluconobacter oxydans DSM 2003. Biotechnol Bioprocess Eng 17:1156–1164. https://doi.org/10.1007/s12257-012-0339-0 CrossRefGoogle Scholar
- White SA, Claus GW (1982) Effect of intracytoplasmic membrane development on oxidation of sorbitol and other polyols by Gluconobacter oxydans. J Bacteriol 150:934–943Google Scholar
- Zhou X, Zhou X, Xu Y (2017a) Improvement of fermentation performance of Gluconobacter oxydans by combination of enhanced oxygen mass transfer in compressed-oxygen-supplied sealed system and cell-recycle technique. Bioresour Technol 244(Part 1):1137–1141. https://doi.org/10.1016/j.biortech.2017.08.107 CrossRefGoogle Scholar