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Determination of lansoprazole, 5-hydroxylansoprazole, and lansoprazole sulfone in human plasma for CYP2C19 and CYP3A4 phenotyping

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Abstract

A simple and sensitive liquid chromatography method with absorbance or mass spectrometry detection was developed and validated for the determination of lansoprazole, lansoprazole sulfone, and 5-hydroxylansorpazole in human serum with omeprazole as an internal standard. Analytes were, after an extraction step with a mixture of isopropyl alcohol: dichloromethane (2:8, v/v), separated in a gradient elution (acetonitrile: 20 mM ammonium formate) and detected at 280 and 300 nm or by monitoring selective reactions in a triple quadrupole mass analyzer. The limits of detection were, for all analytes, below 2 ng/mL and 3 pg/mL in the LC UV and LC MS method, respectively. The combined linear dynamic range exceeded 5 orders of magnitude. The method was successfully applied in the assessment of CYP2C19 and CYP3A4 metabolic phenotypes in ST-elevated myocardial infarction patients receiving novel anticoagulant treatment.

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Acknowledgements

This work was supported by funds from the Faculty of Medicine MU to a junior researcher Ondřej Peš and by the Specific University Research Grant (MUNI/A/1132/2017, MUNI/A/0910/2017) provided by the Ministry of Education, Youth and Sports of the Czech Republic and by the project no. LQ1605 from the National Program of Sustainability II (MEYS CR).

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Medicine, Masaryk University, Kamenice 5, 62500, Brno, Czech Republic

    Katarína Kostolanská & Ondřej Peš

  2. Department of Pharmacology, Faculty of Medicine, Masaryk University, Kamenice 5, 62500, Brno, Czech Republic

    Ondřej Zendulka & Jan Juřica

  3. Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Kamenice 5, 62500, Brno, Czech Republic

    Jan Máchal

  4. Department of Human Pharmacology and Toxicology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho tr. 1946/1, 61242, Brno, Czech Republic

    Jan Juřica

  5. Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, 62500, Brno, Czech Republic

    Jan Juřica

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  1. Katarína Kostolanská
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  2. Ondřej Peš
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  3. Ondřej Zendulka
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  4. Jan Máchal
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Correspondence to Jan Juřica.

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Kostolanská, K., Peš, O., Zendulka, O. et al. Determination of lansoprazole, 5-hydroxylansoprazole, and lansoprazole sulfone in human plasma for CYP2C19 and CYP3A4 phenotyping. Chem. Pap. 73, 2955–2963 (2019). https://doi.org/10.1007/s11696-019-00682-4

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  • DOI: https://doi.org/10.1007/s11696-019-00682-4

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