Kinetics of DNA condensation with DPPC: effect of calcium and sodium cations
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Kinetics of DNA condensation with zwitterionic lipid, dipalmitoylphosphatidylcholine, in the presence of calcium and sodium cations was investigated using fluorescence stopped-flow technique. The process was followed from two points of view: the changes on DNA were monitored with ethidium bromide, and fluorescently labelled phospholipid was used to follow the changes in the lipid bilayer. The measurements showed different reaction kinetics. In the case of the DNA side, a consecutive reaction mechanism was found, while two-step first-order reaction mechanism described well the processes on the lipid side. Both, sodium and calcium cations influence significantly the reaction mechanism. Calcium is necessary to condensate DNA with lipid, while sodium modulates the kinetics of the reactions considerably.
KeywordsLipoplex Reaction kinetics Fluorescent probe Fluorescent label
Authors gratefully acknowledge financial support from the Grant Agency of the Ministry of Education, Science, Research and Sport of the Slovak Republic Grant VEGA 1/0916/16 and from Joint Institute for Nuclear Research in Dubna, Russia JINR Project 04-4-1121-2015/2017.
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