Chemical Papers

, Volume 72, Issue 4, pp 993–1005 | Cite as

Kinetics of DNA condensation with DPPC: effect of calcium and sodium cations

  • Alexander Búcsi
  • Gilda Liskayová
  • Dominika Galliková
  • Daniela Uhríková
Original Paper
  • 36 Downloads

Abstract

Kinetics of DNA condensation with zwitterionic lipid, dipalmitoylphosphatidylcholine, in the presence of calcium and sodium cations was investigated using fluorescence stopped-flow technique. The process was followed from two points of view: the changes on DNA were monitored with ethidium bromide, and fluorescently labelled phospholipid was used to follow the changes in the lipid bilayer. The measurements showed different reaction kinetics. In the case of the DNA side, a consecutive reaction mechanism was found, while two-step first-order reaction mechanism described well the processes on the lipid side. Both, sodium and calcium cations influence significantly the reaction mechanism. Calcium is necessary to condensate DNA with lipid, while sodium modulates the kinetics of the reactions considerably.

Keywords

Lipoplex Reaction kinetics Fluorescent probe Fluorescent label 

Notes

Acknowledgements

Authors gratefully acknowledge financial support from the Grant Agency of the Ministry of Education, Science, Research and Sport of the Slovak Republic Grant VEGA 1/0916/16 and from Joint Institute for Nuclear Research in Dubna, Russia JINR Project 04-4-1121-2015/2017.

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Copyright information

© Institute of Chemistry, Slovak Academy of Sciences 2017

Authors and Affiliations

  1. 1.Department of Physical Chemistry of Drugs, Faculty of PharmacyComenius University in BratislavaBratislavaSlovak Republic

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