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Determination of citrate released from stabilized gold nanoparticles by capillary zone electrophoresis

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Abstract

In this article, we report a pilot study on release of citrate ions from citrate-stabilized gold nanoparticles by capillary zone electrophoresis. First, a method for determination of citrate released from nanoparticles was developed using 5 mM phthalate buffer pH 6.5 with polybrene capillary inner wall coating. Within these conditions, citrate migrated in 1.8 min and it can be determined with LOD of 7 µmol/L using molybdate as the internal standard. The release of citrate was initiated by addition of common MOPS buffer ions used in capillary electrophoresis to nanoparticles samples. Non-linear behavior was found that proves release of citrate from stabilized gold nanoparticles. The release is initiated when 5 mM MOPS is added to nanoparticles’ solution. This behavior can partially explain zeta potential change of the nanoparticles from –34 mV in bulk solution to –28 mV in 50 mM MOPS.

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Acknowledgements

The financial support of the research by the Ministry of Education, Youth, and Sports of the Czech Republic (project NPU LO 1305) and the Czech Science Foundation (project 16-23938Y) is gratefully acknowledged.

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Authors and Affiliations

  1. Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University in Olomouc, 17. listopadu 12, 77146, Olomouc, Czech Republic

    Jana Horská, Juraj Ševčík & Jan Petr

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  1. Jana Horská
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  2. Juraj Ševčík
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  3. Jan Petr
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Correspondence to Jan Petr.

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Horská, J., Ševčík, J. & Petr, J. Determination of citrate released from stabilized gold nanoparticles by capillary zone electrophoresis. Chem. Pap. 72, 419–424 (2018). https://doi.org/10.1007/s11696-017-0291-8

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  • DOI: https://doi.org/10.1007/s11696-017-0291-8

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