Chemical Papers

, Volume 71, Issue 11, pp 2233–2242 | Cite as

Phytochemical profiles and antioxidant capacity of the crude extracts, aqueous- and saponin-enriched butanol fractions of Helicteres hirsuta Lour. leaves and stems

  • Hong Ngoc Thuy PhamEmail author
  • Q. V. Vuong
  • Michael C. Bowyer
  • Christopher J. ScarlettEmail author
Original Paper


This study aimed to compare phytochemical profiles and antioxidant capacity of various extracts including crude extracts, aqueous- and saponin-enriched butanol fractions prepared from the stems and leaves of Helicteres hirsuta Lour. The results revealed that all the three powdered extracts from the leaves and the stems possessed high levels of phenolics (177.07–241.03 mg GAE g−1), flavonoids (158.03–280.06 mg CE g−1) and saponins (165.77–1035.33 mg ESE g−1) and exhibited strong antioxidant capacity. HPLC analysis identified nine major compounds in the leaf powder crude extract; however, the leaf aqueous fraction had three extra compounds; whereas, the saponin-enriched butanol leaf fraction had seven extra compounds. For the stems, twelve main compounds were evident in either the powdered crude extract or the aqueous fraction, and five new compounds were revealed in the saponin-enriched butanol fraction. The findings revealed that the powdered aqueous fractions and saponin-enriched butanol fractions are potential sources of biologically active compounds for further investigation and industrial utilisation.


Helicteres hirsuta Lour. Phytochemical profiles Phenolics Flavonoids Saponins Antioxidant capacity 



We greatly thank the University of Newcastle International Postgraduate Research Scholarship (UNIPRS) and University of Newcastle Research Scholarship Central 50:50 (UNRSC 50:50) that awarded the Ph.D. scholarship to Hong Ngoc Thuy Pham in 2015.


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Copyright information

© Institute of Chemistry, Slovak Academy of Sciences 2017

Authors and Affiliations

  1. 1.School of Environmental and Life Sciences, Faculty of ScienceUniversity of NewcastleOurimbahAustralia
  2. 2.Faculty of Food TechnologyNha Trang UniversityNha Trang CityVietnam

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