Phytochemical profiles and antioxidant capacity of the crude extracts, aqueous- and saponin-enriched butanol fractions of Helicteres hirsuta Lour. leaves and stems
- 140 Downloads
This study aimed to compare phytochemical profiles and antioxidant capacity of various extracts including crude extracts, aqueous- and saponin-enriched butanol fractions prepared from the stems and leaves of Helicteres hirsuta Lour. The results revealed that all the three powdered extracts from the leaves and the stems possessed high levels of phenolics (177.07–241.03 mg GAE g−1), flavonoids (158.03–280.06 mg CE g−1) and saponins (165.77–1035.33 mg ESE g−1) and exhibited strong antioxidant capacity. HPLC analysis identified nine major compounds in the leaf powder crude extract; however, the leaf aqueous fraction had three extra compounds; whereas, the saponin-enriched butanol leaf fraction had seven extra compounds. For the stems, twelve main compounds were evident in either the powdered crude extract or the aqueous fraction, and five new compounds were revealed in the saponin-enriched butanol fraction. The findings revealed that the powdered aqueous fractions and saponin-enriched butanol fractions are potential sources of biologically active compounds for further investigation and industrial utilisation.
KeywordsHelicteres hirsuta Lour. Phytochemical profiles Phenolics Flavonoids Saponins Antioxidant capacity
We greatly thank the University of Newcastle International Postgraduate Research Scholarship (UNIPRS) and University of Newcastle Research Scholarship Central 50:50 (UNRSC 50:50) that awarded the Ph.D. scholarship to Hong Ngoc Thuy Pham in 2015.
- Apak R, Güçlü K, Özyürek M, Karademir SE (2004) Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. J Agric Food Chem 52:7970–7981. doi: 10.1021/jf048741x CrossRefGoogle Scholar
- Chuakul W, Saralamp P, Boonpleng A (2002) Medicinal plants used in the Kutchum District, Yasothon Province, Thailand. Thai J Phytopharm 9:22–49Google Scholar
- Kamonwannasit S, Nantapong N, Kumkrai P, Luecha P, Kupittayanant S, Chudapongse N (2013) Antibacterial activity of Aquilaria crassna leaf extract against Staphylococcus epidermidis by disruption of cell wall. Ann Clinic Microbiol Antimicrob 12:1–7. doi: 10.1186/1476-0711-12-20 CrossRefGoogle Scholar
- Lamson DW, Brignall MS (1999) Antioxidants in cancer therapy; their actions and interactions with oncologic therapies. Altern Med Rev 4:304–329Google Scholar
- Scarlett CJ, Vuong QV, Mccluskey A, Bowyer MC (2015) Pancreatic cancer drugs: case studies in synthesis and production. In: Scarlett CJ, Vuong QV (eds) Plant bioactive compounds for pancreatic cancer prevention and treatment. Nova Science Publishers, New York, pp 145–193Google Scholar
- Yan LL, Zhang YJ, Gao WY, Man SL, Wang Y (2009) In vitro and in vivo anticancer activity of steroid saponins of Paris polyphylla var. yunnanensis. Exp Oncol 31:27–32Google Scholar