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Duodenal-Jejunal Bypass Maintains Gut Permeability by Suppressing Gut Inflammation

Obesity Surgery volume 29, pages 2745–2749 (2019)Cite this article

Abstract

Background

The aim of this study was to investigate gut inflammation and permeability in rats after duodenal-jejunal bypass (DJB) and in rats injected with a glucagon-like peptide-1 (GLP-1) receptor analog.

Methods

Twelve male 16-week-old obese diabetic rats were divided into three groups: the DJB group, the sham group, and the group injected daily with a GLP-1 receptor agonist (liraglutide). Gut inflammation and the expression of tight junction protein (claudin-1) were analyzed in the three groups at 8 weeks after surgery.

Results

The DJB group showed significantly lower levels of gut inflammatory cytokines than the liraglutide group. Claudin-1 showed stronger intensity on immunofluorescent staining in the DJB group than that in the liraglutide group.

Conclusions

In summary, DJB surgery might maintain gut permeability via suppression of gut inflammation.

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Abbreviations

DJB:

Duodenal-jejunal bypass

GLP-1:

Glucagon-like peptide-1

HFD:

High-fat diet

RNA:

Ribonucleic acid

RT:

Reverse transcription

PBS:

Phosphate-buffered salts

DAPI:

4′,6-Diamidino-2-phenylindole

LPS:

Lipopolysaccharide

NASH:

Nonalcoholic steatohepatitis

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Affiliations

  1. Department of Surgery, Tokushima University, 3-18-15 Kuramoto-cho, Tokushima, Tokushima, 770-8503, Japan

    Hideya Kashihara, Mitsuo Shimada, Kozo Yoshikawa, Jun Higashijima, Tomohiko Miyatani, Takuya Tokunaga, Masaaki Nishi & Chie Takasu

Authors
  1. Hideya Kashihara
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  2. Mitsuo Shimada
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  3. Kozo Yoshikawa
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  4. Jun Higashijima
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  5. Tomohiko Miyatani
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  6. Takuya Tokunaga
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  7. Masaaki Nishi
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  8. Chie Takasu
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Corresponding author

Correspondence to Hideya Kashihara.

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All applicable institutional and/or national guidelines for the care and use of animals were followed. Informed consent statement does not apply to this study.

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The authors declare that they have no conflict of interest.

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Kashihara, H., Shimada, M., Yoshikawa, K. et al. Duodenal-Jejunal Bypass Maintains Gut Permeability by Suppressing Gut Inflammation. OBES SURG 29, 2745–2749 (2019). https://doi.org/10.1007/s11695-019-03922-4

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Keywords

  • Gut inflammation
  • Gut permeability
  • Tight junction protein (claudin-1)
  • GLP-1 receptor agonist (liraglutide)
  • Gut inflammatory cytokines (IFN-γ, IL-1, IL-6, and TNF-α)
  • Roux-en-Y reconstruction
  • Bariatric surgery