Duodenojejunal Bypass Plus Sleeve Gastrectomy Reduces Infiltration of Macrophages and Secretion of TNF-α in the Visceral White Adipose Tissue of Goto-Kakizaki Rats

Abstract

Background

Current studies indicate that inflammation of white adipose tissue (WAT) is a pathogenic characteristic of insulin resistance. However, the significance of visceral WAT inflammation after bariatric surgery remains unclear.

Methods

Duodenojejunal bypass plus sleeve gastrectomy (DJB-SG) was performed on Goto-Kakisaki rats. Weight, fasting blood glucose (FBG), and homeostatic model assessment of insulin resistance (HOMA-IR) in the DJB-SG group were compared to those in a sham surgery (SHAM) group every 2 weeks. The results of an oral glucose tolerance test (OGTT) and the volume of visceral adipose tissue (Visc.Fat) were compared before and 8 weeks postsurgery. Eight weeks after surgery, the rats were sacrificed and visceral WAT collected from the greater omentum. Tumor necrosis factor-α (TNF-α) and cluster of differentiation 68 (CD68) expression in the WAT were evaluated in paraffin-embedded sections by immunohistochemistry.

Results

Compared with the SHAM group, the DJB-SG group demonstrated a significant reduction in weight, FBG, and HOMA-IR (P < 0.05), with elevation of insulin levels (P < 0.05) from 4 weeks after surgery. OGTT and the quantity of Visc.Fat were significantly reduced (P < 0.05) 8 weeks after surgery. Moreover, the expression of TNF-α and CD68 in the visceral white adipose tissue was significantly lower 8 weeks after surgery (P < 0.05).

Conclusions

The DJB-SG model established in Goto-Kakisaki rats achieved anticipated efficacy. Reduced TNF-α-related inflammation in visceral WAT may result in improved insulin resistance.

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Funding

This work was supported by the Science and Technology Planning Project of Guangzhou, China (Nos. 201508020002 and 201604020106), and the Science and Technology Planning Project of Guangdong Province, China (No. 2017ZC0324).

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Correspondence to Xiaojiang Dai or Liangping Wu.

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Conflict of Interest

Hao Yu, Zhigao Song, Hongbin Zhang, Kehong Zheng, Junfang Zhan,Jingbo Sun, Zhizhi Wang, Lucas Zellmer, Xiaojiang Dai, Wu Liangping declare that they have no conflicts of interest.

Ethical Approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of Guangzhou General Hospital of Guangzhou Military Command’s research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

All applicable Guangzhou General Hospital of Guangzhou Military Command’s guidelines for the care and use of animals were followed.

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All animal experimental procedures involved in this study were approved by the Animal Care and Utilization Committee of Guangzhou General Hospital of Guangzhou Military Command.

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Yu, H., Song, Z., Zhang, H. et al. Duodenojejunal Bypass Plus Sleeve Gastrectomy Reduces Infiltration of Macrophages and Secretion of TNF-α in the Visceral White Adipose Tissue of Goto-Kakizaki Rats. OBES SURG 29, 1742–1750 (2019). https://doi.org/10.1007/s11695-019-03755-1

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Keywords

  • DJB-SG
  • Insulin resistance
  • Inflammation
  • T2D