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Upregulation of Ghrelin Gene Expression in the Excluded Stomach of Obese Women with Type 2 Diabetes After Roux-en-Y Gastric Bypass in the SURMetaGIT Study

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Abstract

Background

Mechanisms of type 2 diabetes remission (T2Dr) after Roux-en-Y gastric bypass (RYGB) in obese patients appear to involve gastrointestinal hormones.

Objective

The objective of this study is to explore changes in ghrelin plasma levels and ghrelin gastrointestinal gene expression (GHRL) after RYGB, and their relationships to T2Dr.

Setting

In 20 obese women with T2D, before and 3 months after RYGB, we assessed GHRL expression by microarray and quantitative RT-PCR in gastrointestinal biopsy samples and plasma levels of ghrelin.

Results

After RYGB, GHRL expression increased in the excluded stomach (p < 0.05) with no change in other gastrointestinal sites. There were no significant changes in ghrelin plasma levels and no correlations with T2Dr.

Conclusions

After RYGB, over-expression of GHRL gene occurs only in the excluded stomach with no correlation to T2Dr.

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Fig. 1

Abbreviations

GIT:

Gastrointestinal tract

GLP-1:

Glucagon-like peptide-1

GHRL:

Ghrelin gene

HC-FMUSP:

Hospital das Clinicas, Faculdade de Medicina da Universidade de Sao Paulo

RYGB:

Roux-en-Y gastric bypass

T2D:

Type 2 diabetes

T2Dr:

Type 2 diabetes remission

BMI:

Body mass index

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Acknowledgements

We thank Daniele Yumi for the statistical discussion of the data and the late Prof Daniel Giannella Neto who had an important role and participated in this study.

Funding

This study was funded by Fundação de Amparo à Pesquisa do Estado de São Paulo (Fapesp) project (no. 2011/09612–3) and scholarship DD (2013/03246-0) and by the CAPES scholarship.

Author information

Authors and Affiliations

  1. Departamento de Gastroenterologia, Laboratório Metanutri (LIM 35), Faculdade de Medicina FMUSP, Universidade de Sao Paulo, Sao Paulo, SP, Brazil

    Danielle Cristina Fonseca, Priscila Sala, Raquel Susana Torrinhas & Dan Linetzky Waitzberg

  2. Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel

    Joelle Singer & Pierre Singer

  3. Endocrine Institute, Rabin Medical Center, Belinson Hospital, Jabotinski Street, Petach Tikva, Israel

    Joelle Singer & Pierre Singer

Authors
  1. Danielle Cristina Fonseca
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  2. Priscila Sala
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  3. Joelle Singer
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  4. Pierre Singer
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  5. Raquel Susana Torrinhas
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  6. Dan Linetzky Waitzberg
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Corresponding author

Correspondence to Danielle Cristina Fonseca.

Ethics declarations

This study was performed according to the Declaration of Helsinki and the protocol was approved by the local ethics committee (CAPPesq 1011/09) and registered at www.ClinicalTrials.gov (NCT01251016). Written informed consent was obtained from all patients.

Conflicts of Interest

The authors declare that they have no conflict of interest.

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Fonseca, D.C., Sala, P., Singer, J. et al. Upregulation of Ghrelin Gene Expression in the Excluded Stomach of Obese Women with Type 2 Diabetes After Roux-en-Y Gastric Bypass in the SURMetaGIT Study. OBES SURG 28, 877–880 (2018). https://doi.org/10.1007/s11695-017-3098-3

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  • DOI: https://doi.org/10.1007/s11695-017-3098-3

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