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Analysis of Gene Candidate SNP and Ancestral Origin Associated to Obesity and Postoperative Weight Loss in a Cohort of Obese Patients Undergoing RYGB

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Abstract

Introduction

Obesity is the result of a complex interaction between multiple genetic traits and psychological, behavioral, nutritional and environmental factors.

Objectives

The aims of the study were (a) to comparatively evaluate the presence of 20 candidate gene single nucleotide polymorphisms (SNPs) in morbidly obese patients, (2) their association to comorbid conditions and (3) their impact on weight loss after a Roux-en-Y gastric bypass (RYGB).

Patients and Methods

Two hundred forty-nine patients were eligible for this study. Clinical, anthropometric, biochemical and demographic variables were analyzed. Body mass index (BMI) and composition were assessed by bioelectrical impedance. Twenty SNPs were included for analysis.

Results

There were 168 Mexican mestizos (67.5 %) and 81 (32.5 %) patients with other ancestral origin. One hundred fifty-nine (64.1 %) were females. Mean ± SD age of the general cohort was 41.1 ± 11.3 years (17–71). Preoperative mean ± SD BMI was 42.5 ± 6.5 kg/m2. There were no significant differences between mestizo and non-mestizo for most SNPs except for IFI, LIPC, and ST8SIA2. FTO (OR = 1.71; CI95 % = 1.14–2.57; p = 0.008) and APOB (OR = 0.31; CI95 % = 0.14–0.72; p = 0.004) result is statistically associated to high blood pressure and FTO (OR = 2.0; CI95 % = 1.3–3.1; p = 0.001), GNB3 (OR = 2.69; CI95 % = 1.0–7.2; p = 0.04), IFI30 (OR = 2.0; CI95 % = 1.16–3.6; p = 0.01), and MC4R (OR = 1.81; CI95 % = 1.13–2.9; p = 0.01) to type 2 diabetes (T2D). Based on ANOVA analysis, POMC (rs1042571) was the SNP most significantly associated to a higher weight loss after RYGB.

Conclusions

Obese patients have similar SNP frequencies. Several SNP results are statistically associated to high blood pressure and T2D. POMC was significantly associated to a higher surgically induced weight loss.

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Acknowledgements

We would like to express our gratitude to all the members of our clinic (CNOAM) for their commitment and support on this project.

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Authors and Affiliations

  1. The American British Cowdray (ABC) Medical Center, Center for Nutrition, Obesity and Metabolic Alterations (CNOAM), Mexico City, Mexico

    David Velázquez-Fernández, Diana Clavellina-Gaytán, Ramón Vidrio, Eduardo Vidrio, Maureen Mosti, Hugo Sánchez-Aguilar, Donaji Rodriguez & Miguel F. Herrera

  2. Departamento de Cirugía, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Av. Vasco de Quiroga No. 15, Tlalpan, Belisario Domínguez Sección XVI, CP 14080, Ciudad de Mexico, Mexico

    David Velázquez-Fernández, Pablo León & Miguel F. Herrera

  3. National Institute for Genomic Medicine (INMEGEN), Mexico City, Mexico

    Gabriela Mercado-Celis & Jeny Flores-Morales

  4. Laboratorio de Genómica, División de Estudios de Posgrado e Investigación, Facultad de Odontología UNAM, Mexico City, Mexico

    Gabriela Mercado-Celis & Jeny Flores-Morales

Authors
  1. David Velázquez-Fernández
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  2. Gabriela Mercado-Celis
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  8. Hugo Sánchez-Aguilar
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  11. Miguel F. Herrera
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Corresponding author

Correspondence to Miguel F. Herrera.

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Funding Source

This project was supported by a research grant from Johnson & Johnson Medical Mexico.

Conflict of Interest

The manuscript has been read and approved by all authors. All the authors declare that they have no potential conflicts of interest.

Ethical Statement

This protocol was approved by our institutional research committee in humans and followed the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

Consent Statement

Informed consent was obtained from all individual participants included in this study. All informed consent sheets were signed, properly filed, and saved. Samples and clinical data were unidentified for anonymity rights.

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Velázquez-Fernández, D., Mercado-Celis, G., Flores-Morales, J. et al. Analysis of Gene Candidate SNP and Ancestral Origin Associated to Obesity and Postoperative Weight Loss in a Cohort of Obese Patients Undergoing RYGB. OBES SURG 27, 1481–1492 (2017). https://doi.org/10.1007/s11695-016-2501-9

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  • DOI: https://doi.org/10.1007/s11695-016-2501-9

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