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Changes in Glucose Transporters, Gluconeogenesis, and Circadian Clock after Duodenal–Jejunal Bypass Surgery

Obesity Surgery volume 25, pages 635–641 (2015)Cite this article

Abstract

Background

Bariatric surgery improves obesity and ameliorates glucose tolerance. This study was conducted to evaluate circadian clocks, gluconeogenesis, and glucose transport changes in hepatic and intestinal tissues after duodenal–jejunal bypass (DJB) surgery in a rat model.

Methods

Twenty-five rats were randomly assigned to either sham group (10 rats) or DJB group (15 rats). Food intake, body weight, blood glucose, and serum insulin levels were measured. Quantitative RT-PCR, immunoblot, and immunohistochemistry were used to analyze genes and proteins in the liver and intestine.

Results

Food intake and body weight were not different between sham and DJB groups. Blood glucose level was significantly lower in the DJB group compared with that in the sham group. Although not significant, serum insulin level showed an increased tendency in DJB group. DJB induced marked expressions of glucose transporter-2 (GLUT2) in the liver and GLUT2 and sodium-dependent glucose transporter-1 (SGLT1) in the intestine. Gluconeogenic enzymes [phosphoenolpyruvate carboxykinase-1 (Pck1) and glucose-6-phosphatase (G6Pase)] decreased in the liver and increased in the intestine of the DJB group. Circadian transcription factor cryptochrome-1 (Cry1) increased in the liver and decreased in the intestine of the DJB group. Another circadian transcription factor period-2 (Per2) also increased in the liver and decreased in the intestine of the DJB group.

Conclusion

In conclusion, this study suggests the possibility that Cry1 and Per2 may mediate decreased gluconeogenesis in the liver and increased gluconeogenesis in the intestine of the DJB group.

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Acknowledgments

This work was supported by the research fund of Hanyang University (HY-20010-MC).

Conflict of Interest

Mikyung Kim, Young Gil Son, Yu Na Kang, Tae Kyung Ha, and Eunyoung Ha have no conflicts of interest or other financial ties to disclose.

Author information

Affiliations

  1. Department of Biochemistry, Pain Research Center, School of Medicine, Keimyung University, Daegu, South Korea

    Mikyung Kim & Eunyoung Ha

  2. Department of General Surgery, School of Medicine, Keimyung University, Daegu, South Korea

    Young Gil Son

  3. Department of Pathology, School of Medicine, Keimyung University, Daegu, South Korea

    Yu Na Kang

  4. Department of Surgery, College of Medicine, Hanyang University, Seoul, South Korea

    Tae Kyung Ha

Authors
  1. Mikyung Kim
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  2. Young Gil Son
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  3. Yu Na Kang
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  4. Tae Kyung Ha
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  5. Eunyoung Ha
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Corresponding authors

Correspondence to Tae Kyung Ha or Eunyoung Ha.

Additional information

M. Kim and Y.G. Son contributed equally to this work.

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Kim, M., Son, Y.G., Kang, Y.N. et al. Changes in Glucose Transporters, Gluconeogenesis, and Circadian Clock after Duodenal–Jejunal Bypass Surgery. OBES SURG 25, 635–641 (2015). https://doi.org/10.1007/s11695-014-1434-4

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  • DOI: https://doi.org/10.1007/s11695-014-1434-4

Keywords

  • Circadian clock
  • Gluconeogenesis
  • Glucose transporters
  • Duodenal–Jejunal Bypass