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The Effect of PPI Use on Human Gut Microbiota and Weight Loss in Patients Undergoing Laparoscopic Roux-en-Y Gastric Bypass

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Laparoscopic Roux-en-Y gastric bypass (LRYGB) achieves sustainable weight loss possibly by altering the gut microbiota. The effect of a proton pump inhibitor (PPI) on weight loss and the gut microbiota has not been explored. PPI use and the gut microbiota were assessed before and 6 months after LRYGB in eight patients. Bacterial profiles were generated by 16S ribosomal RNA (rRNA) gene sequencing. Prior to LRYGB, PPI users had a higher percent relative abundance (PRA) of Firmicutes compared to nonusers. PPI users at 6 months post-LRYGB had a higher PRA of Firmicutes [48.6 versus 35.6 %, p = nonsignificant (NS)] and a trend toward significantly lower percent excess weight loss (49.3 versus 61.4 %, p = 0.067) compared to nonusers. PPI use post-LRYGB may impair weight loss by modifying gut microbiota.

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Fig. 1
Fig. 2



Percent relative abundance


Proton pump inhibitor


Roux-en-Y gastric bypass


Laparoscopic Roux-en-Y gastric bypass


Percent weight loss


Percent excess weight loss




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This project was supported by a grant from the Colorado Clinical Translational Science Institute Project Grant (5UL1RR025780).

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No conflicts of interest exist for any of the authors.


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Correspondence to Gregory L. Austin.

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Ward, E.K., Schuster, D.P., Stowers, K.H. et al. The Effect of PPI Use on Human Gut Microbiota and Weight Loss in Patients Undergoing Laparoscopic Roux-en-Y Gastric Bypass. OBES SURG 24, 1567–1571 (2014).

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