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Reduction of Intestinal Electrogenic Glucose Absorption After Duodenojejunal Bypass in a Mouse Model

Obesity Surgery volume 23, pages 1361–1369 (2013)Cite this article

Abstract

Background

The role of intestinal glucose absorption in reducing glycemic levels after duodenojejunal bypass (DJB) is unclear. We hypothesized that DJB surgery decreases intestinal electrogenic glucose absorption by sodium glucose co-transporter 1 (SGLT1), leading to decreased glucose absorption and reduced blood glucose. Six groups of C57/BL6 mice were studied (n = 6 each) 2 weeks, 1 month, and 2 months after DJB or sham surgery.

Methods

Daily weight and food intake were measured for 1 month and an oral glucose tolerance test was performed 2 months after surgery. Electrogenic glucose absorption was quantified in an Ussing chamber according to the sodium-dependent increase of short-circuit current (Isc). Intestinal morphology was assessed by hematoxylin and eosin staining. Expression of SGLT1 was determined by quantitative PCR and Western blotting.

Results

The DJB mice lost weight compared with the sham group and exhibited significant adaptive changes, with increased villus height, crypt depth, and villus surface area. Oral glucose absorption was significantly reduced in the DJB mice compared with the sham group. Glucose-induced Isc was significantly lower in the DJB mice than in the sham mice 1 and 2 months after surgery, indicating that SGLT1 activity was reduced after DJB. Transcript levels and protein abundance of SGLT1 after DJB were also decreased compared with the sham group.

Conclusions

DJB surgery reduced intestinal glucose absorption by reducing the activity and expression of the glucose transporter SGLT1, which represents a potential therapeutic target for patients with diabetes.

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Acknowledgments

We thank Wenfeng Song for the technical assistance on the Ussing chamber. We also greatly thank Junjie Gao for the illustrations. This work was supported by the Foundation for Innovative Research Groups of the National Natural Science Foundation of China (no. 81121002) and National Basic Research Program of China (973 Program) (no. 2009CB522403).

Conflict of interest

All contributing authors (Sheng Yan, Fei Sun, Zhiwei Li, Jie Xiang, Yuan Ding, Zhongjie Lu, Yang Tian, Hui Chen, Jinhua Zhang, Yan Wang, Penghong Song, Lin Zhou, and Shusen Zheng) declare that they have no conflicts of interests.

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Affiliations

  1. Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery; First Affiliated Hospital, School of Medicine; Key Laboratory of Combined Multi-Organ Transplantation, Ministry of Public Health; Key Laboratory of Organ Transplantation, Zhejiang University, Hangzhou, Zhejiang Province, 310003, China

    Sheng Yan, Fei Sun, Zhiwei Li, Jie Xiang, Yuan Ding, Zhongjie Lu, Yang Tian, Hui Chen, Jinhua Zhang, Yan Wang, Penghong Song, Lin Zhou & Shusen Zheng

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  1. Sheng Yan
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  2. Fei Sun
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Correspondence to Shusen Zheng.

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Sheng Yan and Fei Sun contributed equally to this paper.

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Yan, S., Sun, F., Li, Z. et al. Reduction of Intestinal Electrogenic Glucose Absorption After Duodenojejunal Bypass in a Mouse Model. OBES SURG 23, 1361–1369 (2013). https://doi.org/10.1007/s11695-013-0954-7

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Keywords

  • Duodenojejunal bypass
  • Bariatric surgery
  • SGLT1
  • Ussing chamber
  • Glucose absorption