Abstract
Peanut skins are rich in procyanidins (PCs) and are shown to have bioactivity properties. It was reported that there were differences between PCs obtained from peanut skins and grape seeds. The aim of the research was to evaluate the chemical structure and antioxidant capacity of PCs gained from peanut skins compared with those from natural grape seeds. PCs from grape seeds were used as PC standard to evaluate those from peanut skins. It was found that the content of PC standard was higher than peanut skins. PCs from peanut skins were mainly composed of A-type PCs with monomeric units of catechin and epicatechin. PC standard was mainly composed of B-type PCs whose monomeric units were epicatechin, epicatechin gallate and catechin. Additionally, the result of FRAP from peanut skins was lower than PC standard (p < 0.05). However, the results of ORAC, ABTS and DPPH methods showed no significant difference between the two groups (p > 0.05), which was consistent with results of cell antioxidant activity. These researches support that peanut skins can be utilized as a potentially abundant and cheap source of PCs which can be great substitutes of grape seeds.





Similar content being viewed by others
Explore related subjects
Discover the latest articles and news from researchers in related subjects, suggested using machine learning.References
V.S. Sobolev, R.J. Cole, Note on utilisation of peanut seed testa. J. Sci. Food Agric. 84(1), 105–111 (2010)
M.L.D.L. Francisco, A.V.A. Resurreccion, Functional components in peanuts. Crit. Rev. Food Sci. Nutr. 48(8), 715 (2008)
R.R. Bansode, P. Randolph, M. Ahmedna, L.L. Williams, J. Yu, Bioavailability and hypolipidemic effects of peanut skin polyphenols. J. Med. Food 18(3), 265–272 (2015)
K. Tomochika, A. Shimizu-Ibuka, T. Tamura, K. Mura, N. Abe, J. Onose, S. Arai, Effects of peanut-skin procyanidin A1 on degranulation of RBL-2H3 cells. Biosci. Biotechnol. Biochem. 75(9), 1644–1648 (2011)
T. Tsujita, T. Shintani, H. Sato, Preparation and characterisation of peanut seed skin polyphenols. Food Chem. 151(4), 15–20 (2014)
A.C. de Camargo, R.A. Mab, G.B. Rasera, S.G. Canniattibrazaca, P.L. Do, V.O. Alvarenga, A.S. Sant’Ana, F. Shahidi, Phenolic acids and flavonoids of peanut by-products: antioxidant capacity and antimicrobial effects. Food Chem. 237, 538–544 (2017)
F. Natella, F. Belelli, V. Gentili, F. Ursini, C. Scaccini, Grape seed proanthocyanidins prevent plasma postprandial oxidative stress in humans. J. Agric. Food Chem. 50(26), 7720 (2002)
R.R. Bansode, P. Randolph, S. Hurley, M. Ahmedna, Evaluation of hypolipidemic effects of peanut skin-derived polyphenols in rats on Western-diet. Food Chem. 135(3), 1659 (2012)
S.P.P. Da, J.A. Laranjinha, V.A. de Freitas, Antioxidant protection of low density lipoprotein by procyanidins: structure/activity relationships. Biochem. Pharmacol. Biochem. Pharmacol. 66(6), 947–954 (2003)
P.D. Maldonado, I. Riverocruz, R. Mata, J. Pedrazachaverrí, Antioxidant activity of A-type proanthocyanidins from Geranium niveum (Geraniaceae). J. Agric. Food Chem. 53(6), 1996–2001 (2005)
M.M. Appeldoorn, M. Sanders, J.P. Vincken, V. Cheynier, C.L. Guernevé, P.C.H. Hollman, H. Gruppen, Efficient isolation of major procyanidin A-type dimers from peanut skins and B-type dimers from grape seeds. Food Chem. 117(4), 713–720 (2009)
M. Trad, C.L. Bourvellec, H.B. Hamda, C.M.G.C. Renard, M. Harbi, Flavan-3-ols and procyanidins in grape seeds: biodiversity and relationships among wild and cultivated vines. Euphytica 213(11), 242 (2017)
M.K. Dudek, V.B. Gliński, M.H. Davey, D. Sliva, S. Kaźmierski, J.A. Gliński, Trimeric and tetrameric A-type procyanidins from peanut skins. J. Nat. Prod. 80(2), 415 (2017)
J.M. Levy, Evaluation of peanut skin extract, grape seed extract, and grape seed extract fractions to reduce populations of select foodborne pathogens. Virginia Tech (2014)
H. Cao, J. Ou, L. Chen, Y. Zhang, T. Szkudelski, D. Delmas, M. Daglia, J. Xiao, Dietary polyphenols and type 2 diabetes: human study and clinical trial. Crit. Rev. Food Sci. Nutr. 59(20), 3371–3379 (2019)
W. Liu, S. Zhao, J. Wang, J. Shi, Y. Sun, W. Wang, G. Ning, J. Hong, R. Liu, Grape seed proanthocyanidin extract ameliorates inflammation and adiposity by modulating gut microbiota in high-fat diet mice. Mol. Nutr. Food Res. 61(9), 1601082 (2017)
W.E. Lewis, G.K. Harris, T.H. Sanders, B.L. White, L.L. Dean, Antioxidant and anti-inflammatory effects of peanut skin extracts. Food Nutr. Sci. 4(8A), 22–32 (2013)
R.H. Liu, K.L. Nehmer, Cellular antioxidant activity (CAA) assay (2011)
Z.Q. Liu, Q.L. Yang, C.S. Zhang, J. Sun, Y. Zhang, S.Q. Wang, Optimization of ultrasonic extraction technique of proanthocyanidin from peanut skin. Adv. Mater. Res. 156–157, 778–784 (2011)
A. Dávalos, A. Carmen Gómezcordovés, B. Bartolomé, Extending applicability of the oxygen radical absorbance capacity (ORAC – Fluorescein) Assay. J. Agric. Food Chem. 52(1), 48–54 (2004)
R. Marfil, R. Giménez, O. Martínez, P.R. Bouzas, J.A. Rufián-Henares, M. Mesías, C. Cabrera-Vique, Determination of polyphenols, tocopherols, and antioxidant capacity in virgin argan oil (Argania spinosa, Skeels). Eur. J. Lipid Sci. Technol. 113(7), 886–893 (2011)
M. Karamac, R. Amarowicz, S. Weidner, S. Abe, F. Shahidi, Antioxidant activity of rye caryopses and embryos extracts. Nahrung, submitted. Czech J. Food Sci. 20, 209–214 (2002)
M. Monagas, I. Garrido, R. Lebrónaguilar, M.C. Gómezcordovés, A. Rybarczyk, R. Amarowicz, B. Bartolomé, Comparative flavan-3-ol profile and antioxidant capacity of roasted peanut, hazelnut, and almond skins. J. Agric. Food Chem. 57(22), 10590–10599 (2009)
K.L. Wolfe, R.H. Liu, Cellular antioxidant activity (CAA) assay for assessing antioxidants, foods, and dietary supplements. J. Agric. Food Chem. 55(22), 8896–8907 (2007)
J.M. Yu, M. Ahmedna, I. Goktepe, Effects of processing methods and extraction solvents on concentration and antioxidant activity of peanut skin phenolics. Food Chem. 90(1–2), 199–206 (2005)
V. Nepote, N.R. Grosso, C.A. Guzmán, Optimization of extraction of phenolic antioxidants from peanut skins. J. Sci. Food Agric. 85(1), 33–38 (2005)
B. Labarbe, V. Cheynier, F. Brossaud, J.M. Souquet, M. Moutounet, Quantitative fractionation of grape proanthocyanidins according to their degree of polymerization. J. Agric. Food Chem. 47(7), 2719–2723 (1999)
L. Gu, M.A. Kelm, J.F. Hammerstone, Z. Zhang, G. Beecher, J. Holden, D. Haytowitz, R.L. Prior, Liquid chromatographic/electrospray ionization mass spectrometric studies of proanthocyanidins in foods. J. Mass Spectrom. 38(12), 1272 (2003)
M.M. Appeldoorn, J.P. Vincken, M. Sanders, P.C.H. Hollman, H. Gruppen, Combined normal-phase and reversed-phase liquid chromatography/ESI-MS as a tool to determine the molecular diversity of A-type procyanidins in peanut skins. J. Agric. Food Chem. 57(14), 6007 (2009)
P.J. Sarnoski, J.V. Johnson, K.A. Reed, J.M. Tanko, S.F. O’Keefe, Separation and characterisation of proanthocyanidins in Virginia type peanut skins by LC–MS n. Food Chem. 131(3), 927–939 (2012)
Y. Ma, A. Kosińska-Cagnazzo, W.L. Kerr, R. Amarowicz, R.B. Swanson, R.B. Pegg, Separation and characterization of soluble esterified and glycoside-bound phenolic compounds in dry-blanched peanut skins by liquid chromatography–electrospray ionization mass spectrometry. J. Agri. Food Chem. 62(47), 11488–11504 (2014)
M.N. Bravo, S. Silva, A.V. Coelho, L.V. Boas, M.R. Bronze, Analysis of phenolic compounds in Muscatel wines produced in Portugal. Anal. Chim. Acta 563(1–2), 84–92 (2006)
I.I. Rockenbach, E. Jungfer, C. Ritter, B. Santiagoschübel, B. Thiele, R. Fett, R. Galensa, Characterization of flavan-3-ols in seeds of grape pomace by CE, HPLC-DAD-MSn and LC-ESI-FTICR-MS. Food Res. Int. 48(2), 848–855 (2012)
A.K. Sandhu, L. Gu, Antioxidant capacity, phenolic content, and profiling of phenolic compounds in the seeds, skin, and pulp of vitis rotundifolia (Muscadine Grapes) as determined by HPLC-DAD-ESI-MS ~ n. J. Agric. Food Chem. 58(8), 4681 (2010)
X.Q. Dong, B. Zou, Y. Zhang, Z.Z. Ge, J. Du, C.M. Li, Preparation of A-type proanthocyanidin dimers from peanut skins and persimmon pulp and comparison of the antioxidant activity of A-type and B-type dimers ☆. Fitoterapia 91(10), 128–139 (2013)
S.C.D.G.C. Provost, Nicolas Vivas, Comparative study of polyphenol scavenging activities assessed by different methods. J. Agric. Food Chem. 47(2), 425–431 (1999)
Acknowledgements
This work was supported by the National Natural Science Foundation of China (Grant No. 31872895), Foundation of State Key Laboratory of Food Science and Technology (SKLF-ZZB-201907) and the national first-class discipline program of Food Science and Technology (JUFSTR20180202).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors have declared no conflict of interest.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Chang, M., Sun, X., Guo, X. et al. Composition and antioxidant study of procyanidins from peanut skins. Food Measure 14, 2781–2789 (2020). https://doi.org/10.1007/s11694-020-00523-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11694-020-00523-z


