Abstract
The current study was planned to investigate the potential of polysaccharides-based nanoemulsion coatings to improve postharvest storage life of apple fruit. As per experimental plan of response surface methodology (RSM), 13 coating formulations were prepared; coatings were applied to fruit and stored at 20 °C for 14 days. The effects of coating on physicochemical, antioxidant capacity (%), total phenolic content and microbial counts in apple fruit and its juice were assed. The developed model showed non-significant (p > 0.05) lack of fit for all evaluated target response variables. Coefficients of determination (R2) ranging from 92.69 to 99.65% were obtained for these responses. The experimental results investigated in this study suggested that the nanoemulsion based on fenugreek and flax polysaccharide preserved overall quality attributes of apple fruit during storage as well as extended shelf life. Based on RSM, a formulation with a blend of 1.5 g fenugreek and 1.0 g flax polysaccharides was predicted to produce the most desirable effects for target responses, and no significant (p > 0.05) difference between experimental and predicted values. Hence, such an ecofriendly natural approach towards enhancing postharvest quality of fruits was explored to promote trade of organic produce and further investigation is needed regarding scaling and pilot-scale validation trials.




Similar content being viewed by others
Explore related subjects
Discover the latest articles and news from researchers in related subjects, suggested using machine learning.References
M.L. Flores-López, M.A. Cerqueira, D.J. de Rodríguez, A.A. Vicente, Perspectives on utilization of edible coatings and nano-laminate coatings for extension of postharvest storage of fruits and vegetables. Food Eng. Rev. 8(3), 292–305 (2016)
X. He, H. Deng, H.-M. Hwang, The current application of nanotechnology in food and agriculture. J. Food Drug Anal. 27, 1 (2018)
M.J. Pitkethly, Nanomaterials–the driving force. Mater. Today 7(12), 20–29 (2004)
J. Rao, D.J. McClements, Food-grade microemulsions, nanoemulsions and emulsions: fabrication from sucrose monopalmitate & lemon oil. Food Hydrocolloids 25(6), 1413–1423 (2011)
T. Mehmood, A. Ahmed, A. Ahmad, M.S. Ahmad, M.A. Sandhu, Optimization of mixed surfactants-based β-carotene nanoemulsions using response surface methodology: an ultrasonic homogenization approach. Food Chem. 253, 179–184 (2018)
X. Li, L. Wang, B. Wang, Optimization of encapsulation efficiency and average particle size of Hohenbuehelia serotina polysaccharides nanoemulsions using response surface methodology. Food Chem. 229, 479–486 (2017)
H. Liu, R. Jian, H. Chen, X. Tian, C. Sun, J. Zhu, Z. Yang, J. Sun, C. Wang, Application of biodegradable and biocompatible nanocomposites in electronics: current status and future directions. Nanomaterials 9(7), 950 (2019)
F. Salehi, Characterization of new biodegradable edible films and coatings based on seeds gum: a review. J. Packag. Technol. Res. 3, 193–201 (2019)
R. Bernacchia, R. Preti, G. Vinci, Chemical composition and health benefits of flaxseed Austin. J. Nutr. Food Sci. 2(8), 1045 (2014)
S.O. Ozkoc, N. Seyhun, Effect of gum type and flaxseed concentration on quality of gluten-free breads made from frozen dough baked in infrared-microwave combination oven. Food Bioprocess Technol. 8(12), 2500–2506 (2015)
F. Rashid, S. Hussain, Z. Ahmed, Extraction purification and characterization of galactomannan from fenugreek for industrial utilization. Carbohydr. Polym. 180, 88–95 (2018)
F. Rashid, Z. Ahmed, S. Hussain, J.-Y. Huang, A. Ahmad, Linum usitatissimum L. seeds: flax gum extraction, physicochemical and functional characterization. Carbohydr. Polym. 215, 29–38 (2019)
AOAC, Official methods of analysis of AOAC International (17th ed.). Gaithersburg, MD, USA., (2006).
G.-C. Yen, H.-Y. Chen, Antioxidant activity of various tea extracts in relation to their antimutagenicity. J. Agric. Food Chem. 43(1), 27–32 (1995)
C. Cheok, N. Chin, Y. Yusof, R. Talib, C. Law, Optimization of total monomeric anthocyanin (TMA) and total phenolic content (TPC) extractions from mangosteen (Garcinia mangostana Linn.) hull using ultrasonic treatments. Industr. Crops Prod. 50, 1–7 (2013)
L. Siroli, F. Patrignani, D.I. Serrazanetti, G. Tabanelli, C. Montanari, F. Gardini, R. Lanciotti, Lactic acid bacteria and natural antimicrobials to improve the safety and shelf-life of minimally processed sliced apples and lamb's lettuce. Food Microbiol. 47, 74–84 (2015)
Ö. Yaman, L. Bayoιndιrlι, Effects of an edible coating and cold storage on shelf-life and quality of cherries. LWT-Food Sci. Technol. 35(2), 146–150 (2002)
N.B. Gol, P.R. Patel, T.R. Rao, Improvement of quality and shelf-life of strawberries with edible coatings enriched with chitosan. Postharvest Biol. Technol. 85, 185–195 (2013)
A.C. Guerreiro, C.M. Gago, M.L. Faleiro, M.G. Miguel, M.D. Antunes, The effect of edible coatings on the nutritional quality of ‘Bravo de Esmolfe’fresh-cut apple through shelf-life. LWT 75, 210–219 (2017)
F. Kittur, N. Saroja, R. Tharanathan, Polysaccharide-based composite coating formulations for shelf-life extension of fresh banana and mango. Eur. Food Res. Technol. 213(4–5), 306–311 (2001)
T.L. Ohashi, L. Pilon, P.C. Spricigo, M. Miranda, D.S. Corrêa, M.D. Ferreira, Postharvest quality of ‘golden’Papayas (Carica papayal) coated with carnauba wax nanoemulsions. Rev. Iberoamericana Tecnología Postcosecha 16(2), 199–209 (2015)
M. Jaiswal, R. Dudhe, P. Sharma, Nanoemulsion: an advanced mode of drug delivery system. 3 Biotech 5(2), 123–127 (2015)
D.S. Shaker, R.A. Ishak, A. Ghoneim, M.A. Elhuoni, Nanoemulsion: a review on mechanisms for the transdermal delivery of hydrophobic and hydrophilic drugs. Sci. Pharm. 87(3), 17 (2019)
M.L. Zambrano-Zaragoza, E. Gutiérrez-Cortez, A. Del Real, R.M. González-Reza, M.J. Galindo-Pérez, D. Quintanar-Guerrero, Fresh-cut red delicious apples coating using tocopherol/mucilage nanoemulsion: effect of coating on polyphenol oxidase and pectin methylesterase activities. Food Res. Int. 62, 974–983 (2014)
I.-H. Kim, Y.A. Oh, H. Lee, K.B. Song, S.C. Min, Grape berry coatings of lemongrass oil-incorporating nanoemulsion. LWT-Food Sci. Technol. 58(1), 1–10 (2014)
M. Vargas, A. Albors, A. Chiralt, C. González-Martínez, Quality of cold-stored strawberries as affected by chitosan–oleic acid edible coatings. Postharvest Biol. Technol. 41(2), 164–171 (2006)
K.S.D. Moraes, C. Fagundes, M.C. Melo, P. Andreani, A.R. Monteiro, Conservation of Williams pear using edible coating with alginate and carrageenan. Food Sci. Technol. 32(4), 679–684 (2012)
N.S. Baraiya, N.B. Gol, T.R. Rao, Influence of polysaccharide-based edible coatings on the shelf life and nutritional quality of tomato fruit. Food 6(1), 22–27 (2012)
A.S.K. Gardesh, F. Badii, M. Hashemi, A.Y. Ardakani, N. Maftoonazad, A.M. Gorji, Effect of nanochitosan based coating on climacteric behavior and postharvest shelf-life extension of apple cv Golab Kohanz. LWT-Food Sci. Technol. 70, 33–40 (2016)
Z. Yang, X. Zou, Z. Li, X. Huang, X. Zhai, W. Zhang, J. Shi, H.E. Tahir, Improved postharvest quality of cold stored blueberry by edible coating based on composite gum Arabic/roselle extract. Food Bioprocess Technol. 12(9), 1537–1547 (2019)
A. Eshetu, A.M. Ibrahim, S.F. Forsido, C.G. Kuyu, Effect of beeswax and chitosan treatments on quality and shelf life of selected mango (Mangifera indica L.) cultivars. Heliyon 5(1), e01116 (2019)
B.G.D.S. Medeiros, A.C. Pinheiro, M.G. Carneiro-da-Cunha, A.A. Vicente, Development and characterization of a nanomultilayer coating of pectin and chitosan–evaluation of its gas barrier properties and application on ‘Tommy Atkins’ mangoes. J. Food Eng. 110(3), 457–464 (2012)
R. Muller, A. Hommoss, J. Pardeike, C. Schmidt, Lipid nanoparticles (NLC) as novel carrier for cosmetics: special features & state of commercialisation. SÖFW-J 133(9), 40–48 (2007)
M. Radi, S. Akhavan-Darabi, H.R. Akhavan, S. Amiri, The use of orange peel essential oil microemulsion and nanoemulsion in pectin-based coating to extend the shelf life of fresh-cut orange. J. Food Process. Preserv. 42(2), e13441 (2018)
N. Azarakhsh, A. Osman, H. Ghazali, C. Tan, N. Mohd Adzahan, Optimization of alginate and gellan-based edible coating formulations for fresh-cut pineapples. Int. Food Res. J. 19(1), 279 (2012)
F. Cappai, J. Benevenuto, L. Ferrão, P. Munoz, Molecular and genetic bases of fruit firmness variation in blueberry—a review. Agronomy 8(9), 174 (2018)
A.C. Guerreiro, C.M. Gago, M.L. Faleiro, M.G. Miguel, M.D. Antunes, The effect of edible coatings on the nutritional quality of ‘Bravo de Esmolfe’fresh-cut apple through shelf-life. LWT-Food Sci. Technol. 75, 210–219 (2017)
A.M. Cavaco, P. Pinto, M.D. Antunes, J.M. da Silva, R. Guerra, ‘Rocha’pear firmness predicted by a Vis/NIR segmented model. Postharvest Biol. Technol. 51(3), 311–319 (2009)
H. Qi, W. Hu, A. Jiang, M. Tian, Y. Li, Extending shelf-life of fresh-cut ‘Fuji’apples with chitosan-coatings. Innov. Food Sci. Emerg. Technol. 12(1), 62–66 (2011)
M.P. Van, N. Chatchavanthatri, H.T. Chi, D.T. Duc, Effect of carboxyl methyl cellulose and gum arabic based edible coating on the quality of sugar apple during storage. Annals. Food Sci. Technol. 19(1), 103–110 (2018)
R. Thakur, P. Pristijono, J. Golding, C.E. Stathopoulos, C. Scarlett, M. Bowyer, S. Singh, Q. Vuong, Development and application of rice starch based edible coating to improve the postharvest storage potential and quality of plum fruit (Prunus salicina). Sci. Hortic. 237, 59–66 (2018)
S. Eshghi, M. Hashemi, A. Mohammadi, F. Badii, Z. Mohammadhoseini, K. Ahmadi, Effect of nanochitosan-based coating with and without copper loaded on physicochemical and bioactive components of fresh strawberry fruit (Fragaria x ananassa Duchesne) during storage. Food Bioprocess Technol. 7(8), 2397–2409 (2014)
C.-Y. Hsu, P.-Y. Chao, S.-P. Hu, C.-M. Yang, The antioxidant and free radical scavenging activities of chlorophylls and pheophytins. Food Nutr. Sci. 4(08), 1 (2013)
A. Mohammadi, M. Hashemi, S.M. Hosseini, Postharvest treatment of nanochitosan-based coating loaded with Zataria multiflora essential oil improves antioxidant activity and extends shelf-life of cucumber. Innov. Food Sci. Emerg. Technol. 33, 580–588 (2016)
M.K. Saba, O.B. Sogvar, Combination of carboxymethyl cellulose-based coatings with calcium and ascorbic acid impacts in browning and quality of fresh-cut apples. LWT-Food Sci. Technol. 66, 165–171 (2016)
S. Valiathan, K. Athmaselvi, Gum arabic based composite edible coating on green chillies. Int. Agrophys. 32(2), 193–202 (2018)
X. Meng, B. Li, J. Liu, S. Tian, Physiological responses and quality attributes of table grape fruit to chitosan preharvest spray and postharvest coating during storage. Food Chem. 106(2), 501–508 (2008)
I.H. Kim, H. Lee, J.E. Kim, K.B. Song, Y.S. Lee, D.S. Chung, S.C. Min, Plum coatings of lemongrass oil incorporating carnauba wax based nanoemulsion. J. Food Sci. 78(10), E1551–E1559 (2013)
W. Li, L. Li, Y. Cao, T. Lan, H. Chen, Y. Qin, Effects of PLA film incorporated with ZnO nanoparticle on the quality attributes of fresh-cut apple. Nanomaterials 7(8), 207 (2017)
A.I. Durrani, S.H. Nasar, U. Bashir, A.K. Durrani, A. Munawar, preparation, characterization and application of vitamin-E fortified nanocoatings on fresh-cut apples. J. Fac. Eng. Technol. 21(2), 46–59 (2014)
C. Zhang, W. Li, B. Zhu, H. Chen, H. Chi, L. Li, Y. Qin, J. Xue, The quality evaluation of postharvest strawberries stored in Nano-Ag packages at refrigeration temperature. Polymers 10(8), 894 (2018)
X. Ruelas-Chacon, J. Contreras-Esquivel, J. Montañez, A. Aguilera-Carbo, M. Reyes-Vega, R. Peralta-Rodriguez, G. Sanchéz-Brambila, Guar gum as an edible coating for enhancing shelf-life and improving postharvest quality of roma tomato (Solanum lycopersicum L), J. Food Qual. 2017 (2017).
A.E. Hegazy, The effect of edible coating on the quality attributes and shelf life of persimmon fruit. Curr. Sci. Int. 06(04), 880–890 (2017)
F. Dong, S. Li, Z. Liu, K. Zhu, X. Wang, C. Jin, Improvement of quality and shelf life of strawberry with nanocellulose/chitosan composite coatings. Bangladesh J. Bot. 44(5), 709–717 (2015)
M.R. Moreira, L. Cassani, O. Martín-Belloso, R. Soliva-Fortuny, Effects of polysaccharide-based edible coatings enriched with dietary fiber on quality attributes of fresh-cut apples. J. Food Sci. Technol. 52(12), 7795–7805 (2015)
O. Chauhan, P. Raju, A. Singh, A. Bawa, Shellac and aloe-gel-based surface coatings for maintaining keeping quality of apple slices. Food Chem. 126(3), 961–966 (2011)
L. Pilon, P.C. Spricigo, M. Miranda, M.R. de Moura, O.B.G. Assis, L.H.C. Mattoso, M.D. Ferreira, Chitosan nanoparticle coatings reduce microbial growth on fresh-cut apples while not affecting quality attributes. Int. J. Food Sci. Technol. 50(2), 440–448 (2015)
L. Salvia-Trujillo, M.A. Rojas-Graü, R. Soliva-Fortuny, O. Martín-Belloso, Use of antimicrobial nanoemulsions as edible coatings: impact on safety and quality attributes of fresh-cut Fuji apples. Postharvest Biol. Technol. 105, 8–16 (2015)
Acknowledgements
We are highly thankful to Higher Education Commission of Pakistan (HEC) for providing all fundings to complete this research in the form Indigenous PhD Scholarship.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
All Authors have no conflict of interest to declare.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Rashid, F., Ahmed, Z., Ameer, K. et al. Optimization of polysaccharides-based nanoemulsion using response surface methodology and application to improve postharvest storage of apple (Malus domestica). Food Measure 14, 2676–2688 (2020). https://doi.org/10.1007/s11694-020-00514-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11694-020-00514-0


