Potential antiglycation, antioxidant and antiproliferative activities of Vicia faba peptides

Abstract

The elevated blood sugar slowly attacks reactive amino group of functional proteins and impairs its functions, generates reactive oxygen species (ROS) as byproduct and increases risk of cancer. Bioactive peptides might act as protecting agent via trapping excess sugar. In this study, the bioactivity of peptides from Vicia faba by tryptic digestion was evaluated as antiglycation, antioxidant and antiproliferative assays. Results obtained indicated that V. faba peptides fractions < 3 kDa trapped extra glucose and fructose, inhibited in vitro glycation up to 49.5%, protected bovine serum albumin from glycation induced fibril formation, neutralized DPPH free radical and exhibited a cytotoxic effect against PC3, MCF-7 and HepG2 cell lines with a significant decrease in cell viability after 48 h of incubation (IC50 0.54 mg/ml to 7.58 mg/ml). Vicia faba peptides promising consumed as nutritional supplements due to its effective biological protection against diabetic complications and anticancer agent. Peptides obtained from Vicia faba were tested as antiglycation, antioxidants and antitumor activity against different cell lines. Different techniques and methods used including separation and identification of peptides from V. faba, peptides reactivity with reducing sugars, measuring peptides, measuring free sugars, antiglycation activity, MTT assay, DPPH radical assay and anti-proliferative effect of obtained peptides. Vicia faba could have the potential use as food ingredients or nutritional supplements due to its encapsulation of bioactive peptides.

Graphic abstract