Skip to main content
Springer Nature Link
Log in

Characterization, antioxidant and protective effects of edible Rumex roseus on erythrocyte oxidative damage induced by methomyl

  • Original Paper
  • Published:
Journal of Food Measurement and Characterization Aims and scope Submit manuscript

Abstract

The present work explored the characterization, antioxidant and protective effects of edible Rumex roseus on erythrocyte oxidative damage induced by methomyl in mice. In a preliminary in vitro study, luteolin and ferulic acid were detected, using HPLC–DAD, as the most abundant phenols; Rumex roseus extract showed also a promising antioxidant effect. In the in vivo experiment, mice were subjected to methomyl intoxication demonstrating a significant decrease in red blood cells, hemoglobin and hematocrit levels coupled by a significant increase in bilirubin, LDH, osmotic fragility, reticulocyte and white blood cells number. Erythrocyte specific alterations were also detected as sphercytosis, schistocytosis, dacryocytosis and Howell-jolly bodies; these features are characteristic of a normocytic normochromic regenerative anemia. The oxidative status was also altered, showing a significant increase in malondialdehyde concentration, GSH level and SOD activity while CAT and GPx activities significantly decreased. All the above-cited alterations showed signs of reversal when mice received the methanolic extract of Rumex roseus. The present data suggest that Rumex roseus could be classified as a novel functional food due to its dual role; further to being traditionally used as a flavor food, it was proven by the present work that the new Rumex was capable to alleviate mice erythrocyte oxidative stress known as one of the most critical health issues.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+
from $39.99 /Month
  • Starting from 10 chapters or articles per month
  • Access and download chapters and articles from more than 300k books and 2,500 journals
  • Cancel anytime
View plans

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

Explore related subjects

Discover the latest articles, books and news in related subjects, suggested using machine learning.

References

  1. A. Savran, G. Zengin, A. Aktumsek, A. Mocan, J. Glamoćlija, A. Ćirić, M. Soković, Phenolic compounds and biological effects of edible Rumex scutatus and Pseudosempervivum sempervivum: potential sources of natural agents with health benefits. Food Funct. 7, 3252–3262 (2016)

    CAS  PubMed  Google Scholar 

  2. A. Prakash Mishra, M. Sharifi-Rad, M.A. Shariati, Y.N. Mabkhot, S.S. Al-Showiman, A. Rauf, B. Salehi, M. Župunski, M. Sharifi-Rad, P. Gusain, J. Sharifi-Rad, H.A.R. Suleria, M. Iriti, Bioactive compounds and health benefits of edible Rumex species-a review. Cell Mol. Biol. (Noisy-le-grand) 64, 27–34 (2018)

    Google Scholar 

  3. A. Yashin, Y. Yashin, X. Xia, B. Nemzer, Antioxidant activity of spices and their impact on human health: a review. Antioxidants 6, E70 (2017)

    PubMed  Google Scholar 

  4. D.F.I. Ahmed, Effect of sodium carbonate, Eruca Sativa oil, lavender oil and Aloe vera oil on liver in breast cancer induced female rats treated with doxorubicin. Int. J. Adv. Res. 6, 1305–1317 (2018)

    CAS  Google Scholar 

  5. S.D.S. Banjarnahor, N. Artanti, Antioxidant properties of flavonoids. Med. J. Indones. 23, 239 (2014)

    Google Scholar 

  6. A. Vasas, O. Orbán-Gyapai, J. Hohmann, The genus Rumex: review of traditional uses, phytochemistry and pharmacology. J. Ethnopharmacol. 175, 198–228 (2015)

    CAS  PubMed  Google Scholar 

  7. F. An, S. Wang, D. Yuan, Y. Gong, S. Wang, Attenuation of oxidative stress of erythrocytes by plant-derived flavonoids, orientin and luteolin. Evid. Based Complement. Alternat. Med. 2016, 3401269 (2016)

    PubMed  PubMed Central  Google Scholar 

  8. I. Ghorbel, S. Maktouf, C. Kallel, S. Ellouze Chaabouni, T. Boudawara, N. Zeghal, Disruption of erythrocyte antioxidant defense system, hematological parameters, induction of pro-inflammatory cytokines and DNA damage in liver of co-exposed rats to aluminium and acrylamide. Chem. Biol. Interact. 236, 31–40 (2015)

    CAS  PubMed  Google Scholar 

  9. M. Fenech, The role of folic acid and Vitamin B12 in genomic stability of human cells. Mutat. Res. 475, 57–67 (2001)

    CAS  PubMed  Google Scholar 

  10. G. Dhaliwai, P.A. Cornett, L.M. Tierney, Hemolytic anemia. Am. Fam. Phys. 69, 2599–2606 (2004)

    Google Scholar 

  11. C. Robier, D. Klescher, G. Reicht, O. Amouzadeh-Ghadikolai, F. Quehenberger, M. Neubauer, Dacryocytes are a common morphologic feature of autoimmune and microangiopathic haemolytic anaemia. Clin. Chem. Lab. Med. 53, 1073–1076 (2015)

    CAS  PubMed  Google Scholar 

  12. I.I. Abdel Aziz, B.M. Zabut, Alterations of blood indices associated with low-dose administration of methomyl insecticide for short times to albino rats. Egypt J. Biol. 16, 17–23 (2014)

    Google Scholar 

  13. J.M. Toledo, L.E. Gamalo, M.D. Maligalig, K.J. Suetos, M. Galapon, Estrous cycle and early pregnancy of white mice exposed to methomyl. Pollution 5, 279–286 (2019)

    CAS  Google Scholar 

  14. M. Araoud, F. Neffeti, W. Douki, H.B. Hfaiedh, M. Akrout, M. Hassine, M.F. Najjar, A. Kenani, Adverse effects of pesticides on biochemical and haematological parameters in Tunisian agricultural workers. J. Expos. Sci. Environ. Epidemiol. 22, 243–247 (2012)

    CAS  Google Scholar 

  15. S.L. Meng, J.Z. Chen, P. Xu, J.H. Qu, L.M. Fan, C. Song, L.P. Qiu, Hepatic antioxidant enzymes SOD and CAT of Nile tilapia (Oreochromis niloticus) in response to pesticide methomyl and recovery pattern. Bull. Environ. Contam. Toxicol. 92, 388–392 (2014)

    CAS  PubMed  Google Scholar 

  16. M.B. Ashour, O.M. Ahmed, A.M.A. Asran, M.A. Ali, Assessment of the preventive effects of salvia officinalis and Ruta graveolens ethanolic leaf extracts on chlorpyrifos- and methomyl-induced renal toxicity and oxidative stress in albino rats. Int. J. Prev. Med. Treat. 6, 34 (2017)

    Google Scholar 

  17. M. Messarah, M.S. Boulakoud, A. Boumendjel, C. Abdennour, A. El Feki, The impact of thyroid activity variations on some oxidizing-stress parameters in rats. C. R. Biol. 330, 107–112 (2007)

    CAS  PubMed  Google Scholar 

  18. A. Djeffal, M. Messarah, A. Boumendjel, L. Kadeche, A.E. Feki, Protective effects of vitamin C and selenium supplementation on methomyl-induced tissue oxidative stress in adult rats. Toxicol. Ind. Health 31, 31–43 (2015)

    CAS  PubMed  Google Scholar 

  19. M. Chaieb, M. Boukhris, Flore succinte et illustrée des zones arides et sahariennes de Tunisie. L’Or du temps (1998)

  20. A. Tomaino, M. Martorana, T. Arcoraci, D. Monteleone, C. Giovinazzo, A.A. Saija, Antioxidant activity and phenolic profile of pistachio (Pistacia vera L., variety Bronte) seeds and skins. Biochimie 92, 1115–1122 (2010)

    CAS  PubMed  Google Scholar 

  21. J. ZhiShen, T. Meng Cheng, W.J. Anming, The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 64, 555–559 (1999)

    CAS  Google Scholar 

  22. A. Kumaran, R. Karunakaran, In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India. LWT Food Sci. Technol. 40, 344–352 (2007)

    CAS  Google Scholar 

  23. A.E. Hagerman, L.G. Butler, Protein precipitation method for the quantitative determination of tannins. J. Agric. Food Chem. 26, 809–812 (1978)

    CAS  Google Scholar 

  24. R.B. Broadhurst, W.T. Jones, Analysis of condensed tannins using acidified vanillin. J. Sci. Food Agric. 29, 788–794 (1978)

    CAS  Google Scholar 

  25. A.A. Elzaawely, T.D. Xuan, H. Koyama, S. Tawata, Antioxidant activity and contents of essential oil and phenolic compounds in flowers and seeds of Alpinia zerumbet (Pers.). Food Chem. 104, 1648–1653 (2007)

    CAS  Google Scholar 

  26. L. Siracusa, A. Saija, M. Cristani, F. Cimino, M. D’Arrigo, D. Trombetta, F. Rao, G. Ruberto, Phytocomplexes from liquorice (Glycyrrhiza glabra L.) leaves—chemical characterization and evaluation of their antioxidant, anti-genotoxic and anti-inflammatory activity. Fitoterapia 82, 546–556 (2011)

    CAS  PubMed  Google Scholar 

  27. J. Buenger, H. Ackermann, A. Jentzsch, A. Mehling, I. Pfitzner, K.A. Reiffen, K.R. Schroeder, U. Wollenweber, An interlaboratory comparison of methods used to assess antioxidant potentials. Int. J. Cosmet. Sci. 28, 135–146 (2006)

    CAS  PubMed  Google Scholar 

  28. H.M. El-bendary, Biochemical effects of methomyl on experimental animals. Appl. Sci. Rep. 5, 74–78 (2014)

    Google Scholar 

  29. M. Sinha, P. Manna, P.C. Sil, A 43 kD protein from the herb, Cajanus indicus L., protects against fluoride induced oxidative stress in mice erythrocytes. Pathophysiology 14, 47–54 (2007)

    CAS  PubMed  Google Scholar 

  30. J.V. Dacie, S.M. Lewis, Practical haematology, 7th edn. (Churchil Livingstone, Edinburgh, 1991), pp. 54–79

    Google Scholar 

  31. H. Mineo, H. Hara, Structure-dependent and receptor-independent increase in osmotic fragility of rat erythrocytes by short-chain fatty acids. Biochem. Biophys. Acta. 1(713), 113–117 (2005)

    Google Scholar 

  32. W.H. Daughaday, O.H. Lowry, N.J. Roskbrough, W.S. Fields, Protein measurement with the Folin phenol reagent. J. Lab. Clin. Med. 39, 663–665 (1952)

    CAS  PubMed  Google Scholar 

  33. H.H. Draper, M. Hadley, Malondialdehyde determination as index of lipid Peroxidation. Methods Enzymol. 186, 421–431 (1990)

    CAS  PubMed  Google Scholar 

  34. L.B. Pereira, C.M.A. de Mazzanti, D. Cargnelutti, L.V. Rossato, J.F. Gonçalves, N. Calgaroto, V. Dressler, F.T. Nicoloso, L.C. Federizzi, V.M. Morsch, M.R. Schetinger, Differential responses of oat genotypes: oxidative stress provoked by aluminum. Biometals 24, 73–83 (2011)

    CAS  PubMed  Google Scholar 

  35. D.J. Jollow, J.R. Mitchell, N. Zampaglione, J.R. Gillette, Bromobenzene-induced liver necrosis. Protective role of glutathione and evidence for 3,4-bromobenzene oxide as the hepatotoxic metabolite. Pharmacology 11, 151–169 (1974)

    CAS  PubMed  Google Scholar 

  36. H. Aebi, Catalase in vitro. Methods Enzymol. 105, 121–126 (1984)

    CAS  PubMed  Google Scholar 

  37. L. Flohé, W.A. Günzler, Assays of glutathione peroxidase. Methods Enzymol. 105, 114–121 (1984)

    PubMed  Google Scholar 

  38. C. Beauchamp, I. Fridovich, Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal. Biochem. 44, 276–287 (1971)

    CAS  PubMed  Google Scholar 

  39. I. Coruh, A. Gormez, S. Ercisli, M. Sengul, Total phenolic content, antioxidant, and antibacterial activity of Rumex crispus grown wild in Turkey. Pharm. Biol. 46, 634–638 (2008)

    CAS  Google Scholar 

  40. A.A. Elzaawely, T.D. Xuan, S. Tawata, Antioxidant and antibacterial activities of Rumex japonicus H OUTT. Aerial Parts. Biol. Pharm. Bull. 28, 2225–2230 (2005)

    CAS  PubMed  Google Scholar 

  41. C. Sarikurkcu, S. Targan, M.S. Ozer, B. Tepe, Fatty acid composition, enzyme inhibitory, and antioxidant activities of the ethanol extracts of selected wild edible plants consumed as vegetables in the Aegean region of Turkey. Int. J. Food Prop. 20, 560–572 (2017)

    CAS  Google Scholar 

  42. F. Beddou, C. Bekhechi, R. Ksouri, D.C. Sari, F.A. Bekkara, Potential assessment of Rumex vesicarius L. as a source of natural antioxidants and bioactive compounds. J. Food Sci. Technol. 52, 3549–3560 (2015)

    CAS  PubMed  Google Scholar 

  43. S.M.Y. Al-Sunafi, Pharmacognostical study of Rumex nervosus Vahl. family (Polygonaceae) growing in Yemen. CU Theses (2016)

  44. D.E. Stevenson, R.D. Hurst, Polyphenolic phytochemicals—just antioxidants or much more? Cell. Mol. Life Sci. 64, 2900–2916 (2007)

    CAS  PubMed  Google Scholar 

  45. S.F. Nabavi, N. Braidy, O. Gortzi, E. Sobarzo-Sanchez, M. Daglia, K. Skalicka-Woźniak, S.M. Nabavi, Luteolin as an anti-inflammatory and neuroprotective agent: a brief review. Brain Res. Bull. 119, 1–11 (2015)

    CAS  PubMed  Google Scholar 

  46. H. Boz, Ferulic acid in cereals—a review. Czech J. Food Sci. 33, 1–7 (2015)

    CAS  Google Scholar 

  47. K. Sevgi, B. Tepe, C. Sarikurkcu, Antioxidant and DNA damage protection potentials of selected phenolic acids. Food Chem. Toxicol. 77, 12–21 (2015)

    CAS  PubMed  Google Scholar 

  48. S.M. Nabavi, M.A. Ebrahimzadeh, S.F. Nabavi, A. Hamidinia, A.R. Bekhradnia, Determination of antioxidant activity, phenol and flavonoid content of Parrotia persicamey. Pharmacologyonline 2, 560–567 (2008)

    Google Scholar 

  49. H. Bouaziz, H. Fetoui, S. Ketata, K. Jammoussi, F. Ellouze, N. Zeghal, Effects of sodium fluoride ingested by lactating mice on some haematological parameters in suckling pups and dams. Res. Rep. Fluoride. 39, 211–219 (2006)

    CAS  Google Scholar 

  50. F.M. El-Demerdash, M.I. Yousef, F.S. Kedwany, H.H. Baghdadi, Role of α-tocopherol and β-carotene in ameliorating the fenvalerate-induced changes in oxidative stress, hemato-biochemical parameters, and semen quality of male rats. J. Environ. Sci. Health B 39, 443–459 (2004)

    CAS  PubMed  Google Scholar 

  51. M.I. Yousef, Aluminium-induced changes in hemato-biochemical parameters, lipid peroxidation and enzyme activities of male rabbits: protective role of ascorbic acid. Toxicology 199, 47–57 (2004)

    CAS  PubMed  Google Scholar 

  52. H. El-Zahar, E.E. Tharwat, W. Abd El-Aal, M.A. El- Ashry, M.M. Saad, S.O. Amin, Rabbits and aflatoxins. 2. Reproductive performance of mature New Zealand white rabbit bucks treated orally with aflatoxins. Egypt J. Rabbit Sci. 6, 67–78 (1996)

    Google Scholar 

  53. S.V. Rana, R. Singh, S. Verma, Protective effects of few antioxidants on liver function in rats treated with cadmium and mercury. Indian J. Exp. Biol. 34, 177–179 (1996)

    CAS  PubMed  Google Scholar 

  54. C. Makrides, M. Koukouvas, G.A. Chillews, S.T. Sikkos, E. Vounou, M. Symeonides, P. Christodoulides, M. Ioannides, Methomyl-induced severe acute pancreatitis: possible etiological association. JOP 6, 166–171 (2005)

    PubMed  Google Scholar 

  55. F. El-Demerdash, A.A. Attia, R.H. Elmazoudy, Biochemical and histopathological changes induced by different time intervals of methomyl treatment in mice liver. J. Environ. Sci. Health A Toxic Hazard. Subst. Environ. Eng. 47, 1948–1954 (2012)

    CAS  Google Scholar 

  56. H. Fétoui, E.M. Garoui, N. Zeghal, Lambda-cyhalothrin-induced biochemical and histopathological changes in the liver of rats: ameliorative effect of ascorbic acid. Exp. Toxicol. Pathol. 61, 189–196 (2009)

    PubMed  Google Scholar 

  57. S. Mongi, M. Mahfoud, B. Amel, J. Kamel, E.F. Abdelfattah, Protective effects of vitamin C against haematological and biochemical toxicity induced by deltamethrin in male Wistar rats. Ecotoxicol. Environ. Safe 74, 1765–1769 (2011)

    CAS  Google Scholar 

  58. O. Lohitnavy, P. Sinhaseni, Increase in lactate dehydrogenase isoenzyme-4 and splenocyte toxicity in methomyl-treated rats. Arhiv za higijenu rada i toksikologiju 49, 231–238 (1998)

    CAS  PubMed  Google Scholar 

  59. N. Soudani, I. Ben Amara, M. Sefi, T. Boudawara, N. Zeghal, Effects of selenium on chromium (VI)-induced hepatotoxicity in adult rats. Exp. Toxicol. Pathol. 63, 541–548 (2011)

    CAS  PubMed  Google Scholar 

  60. H. Ben Saad, I. Nasri, A. Elwej, N. Krayem, R. Jarraya, C. Kallel, N. Zeghal, I. Ben Amara, Mineral and antioxidant-rich extract from the red marine algae Alsidium corallinum exhibits cytoprotective effects against potassium bromate-induced erythrocyte oxidative damages in mice. Biol. Trace Elem. Res. 160, 85–96 (2014)

    CAS  PubMed  Google Scholar 

  61. D.P. Garg, A.K. Bansal, A. Malhotra, R. Kiran, D.K. Dhawan, Methomyl induced hematological and biochemical alterations—protection by vitamin E. Pestic. Biochem. Phys. 93, 127–132 (2009)

    CAS  Google Scholar 

  62. B.J. Bain, Howell-Jolly bodies in acute hemolytic anemia. Am. J. Hematol. 92, 473 (2017)

    PubMed  Google Scholar 

  63. D.P. Garg, R. Kiran, A.K. Bansal, A. Malhotra, D.K. Dhawan, Role of Vitamin E in mitigating methomyl induced acute toxicity in blood of male Wistar rats. Drug Chem. Toxicol. 31, 487–499 (2008)

    CAS  PubMed  Google Scholar 

  64. S.A. Heleno, A. Martins, M.J. Queiroz, I.C. Ferreira, Bioactivity of phenolic acids: metabolites versus parent compounds: a review. Food Chem. 173, 501–513 (2015)

    CAS  PubMed  Google Scholar 

  65. J. Lenzi, A.F. Rodrigues, A. de Sousa Rós, A.B. de Castro, D.D. de Lima, D.D. Magro, A.L. Zeni, Ferulic acid chronic treatment exerts antidepressant-like effect: role of antioxidant defense system. Metab. Brain Dis. 30, 1453–1463 (2015)

    CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This work is part of a doctoral thesis by Meryam CHELLY. We thank the Ministry of Higher Education and Scientific Research, Tunisia, for their financial support. The authors are equally grateful to Professor Hatem REBAI, translator and English professor for having proofread the manuscript.

Author information

Authors and Affiliations

  1. Laboratory of Toxicology-Microbiology Environmental and Health, LR17ES06, Sfax Faculty of Sciences, University of Sfax, BP 1171, 3000, Sfax, Tunisia

    Meryam Chelly, Sabrine Chelly, Hanen Sellami, Radhouane Gdoura & Hanen Bouaziz-Ketata

  2. Laboratory of Organic Chemistry LR17ES08 (Natural Substances Team), Faculty of Sciences of Sfax, University of Sfax, Sfax, Tunisia

    Hichem Ben Salah & Noureddine Allouche

  3. Laboratory of Biodiversity and Aquatic Ecosystems Ecology and Planktonology, Department of Life Sciences, FSS, University of Sfax-Tunisia, Street of Soukra Km 3.5, BP 1171, CP 3000, Sfax, Tunisia

    Khaled Athmouni

  4. Department of Clinical and Experimental Medicine, University of Messina, 98125, Messina, Italy

    Alessandra Bitto

  5. Laboratory of Treatment and Valorization of Water Rejects (LTVRH), Water Researches and Technologies Center (CERTE), University of Carthage, BP 273-8020, Tourist route Soliman, Tunisia

    Hanen Sellami

  6. Hematology Laboratory, CHU Habib Bourguiba, University of Sfax, 3029, Sfax, Tunisia

    Choumous Kallel

Authors
  1. Meryam Chelly
    View author publications

    Search author on:PubMed Google Scholar

  2. Sabrine Chelly
    View author publications

    Search author on:PubMed Google Scholar

  3. Hichem Ben Salah
    View author publications

    Search author on:PubMed Google Scholar

  4. Khaled Athmouni
    View author publications

    Search author on:PubMed Google Scholar

  5. Alessandra Bitto
    View author publications

    Search author on:PubMed Google Scholar

  6. Hanen Sellami
    View author publications

    Search author on:PubMed Google Scholar

  7. Choumous Kallel
    View author publications

    Search author on:PubMed Google Scholar

  8. Noureddine Allouche
    View author publications

    Search author on:PubMed Google Scholar

  9. Radhouane Gdoura
    View author publications

    Search author on:PubMed Google Scholar

  10. Hanen Bouaziz-Ketata
    View author publications

    Search author on:PubMed Google Scholar

Corresponding author

Correspondence to Hanen Sellami.

Ethics declarations

Conflict of interest

The authors report that they have no conflict of interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chelly, M., Chelly, S., Ben Salah, H. et al. Characterization, antioxidant and protective effects of edible Rumex roseus on erythrocyte oxidative damage induced by methomyl. Food Measure 14, 229–243 (2020). https://doi.org/10.1007/s11694-019-00285-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11694-019-00285-3

Keywords

Profiles

  1. Noureddine Allouche View author profile