Potential of hyperspectral imaging for nondestructive determination of chlorogenic acid content in Flos Lonicerae
Chlorogenic acid (CGA), as a major active component, is an important index for evaluating the quality of FlosLonicerae. Hyperspectral imaging (HSI) technology was applied for nondestructive estimating CGA content in FlosLonicerae. In order to obtain the best performance of calibration models, nine different pretreatment methods were investigated and compared based on partial least squares regression (PLSR) models. The optimal method was determined as a standard normal variable (SNV) method with RP2 of 0.9766 and RMSEP of 2.711 for further analysis. To simplify calibration models, different variables selection methods, including the uninformative variable elimination (UVE), successive projections algorithm (SPA), competitive adaptive reweighted sampling (CARS), UVE–CARS, UVE–SPA, CARS–SPA, and UVE–CARS–SPA, were used to extracted characteristic wavelengths from the full spectrum. And then PLSR and least squares support vector machine (LS-SVM) were established based on full spectrum and the selected characteristic wavelengths, respectively. The results showed that the nonlinear UVE–CARS–LS–SVM model (RP2 = 0.9785 and RMSEP = 2.496) was the optimal model for predicting CGA content in FlosLonicerae. Therefore, this study revealed that the combination of HSI with SNV preprocessing method, UVE–CARS variable selection method and LS-SVM modeling had great potential to nondestructively and rapidly determine CGA content in Flos Lonicerae during storage.
KeywordsHyperspectral imaging Flos Lonicerae Chlorogenic acid content Variables selection
The authors express their sincere appreciation to the College Scientific and Technological Innovation Talents Program of Henan province (Project 19HASTIT013), the Natural Science Foundation of Henan Province (Project 162300410100) and the Science and Technology Project of Henan Province (Project 172102310617) for supporting this study financially.
- 1.H. Jiang, L. Yang, X.D. Xing, M.L. Yan, B.Y. Yang, L. Yang, M.Y. Cui, Q.H. Wang, H.X. Kuang, Development of a newly and friendly method to evaluate of phenolic compounds from Flos Lonicerae Japonicae by ultra-high performance supercritical fluid chromatography (UHPSFC) combined with chemometrics. Anal. Methods 10, 4292–4300 (2018)CrossRefGoogle Scholar
- 10.Committee for the Pharmacopoeia of PR China, Pharmacopoeia of the PR China (China Medical Science and Technology Press, Beijing, 2015)Google Scholar
- 24.S. Khoshnoudi-Nia, M. Moosavi-Nasab, S.M. Nassiri, Z. Azimifar, Determination of total viable count in rainbow-trout fish fillets based on hyperspectral imaging system and different variable selection and extraction of reference data methods. Food Anal. Methods 11(12), 3481–3494 (2018)CrossRefGoogle Scholar