Antiproliferative and antioxidative activities of cuttlefish (Sepia pharaonis) protein hydrolysates as affected by degree of hydrolysis

  • Ali Hamzeh
  • Masoud RezaeiEmail author
  • Saber Khodabandeh
  • Ali Motamedzadegan
  • Mehrdad Noruzinia
Original Paper


Bioactivities (including antioxidative and antiproliferative properties) of cuttlefish mantle protein hydrolysates (CPH) with the degree of hydrolysis (DH) of 20.9, 25.5, 30.6, 35.3 and 40.6% (shortened as 20, 25, 30, 35 and 40%, respectively) prepared using alcalase were evaluated. The results indicated that the CPH with 20, 30 and 40% DH showed the greatest activity against DPPH radical scavenging [5.2 µmol TE (torolox equivalent)/g sample], reducing power (0.4 absorbance at 700 nm) and total antioxidant capacity (0.6 mg ascorbic acid equivalent/g sample), which were 2.5, 6.5 and 13.8 times higher than the cuttlefish mantle protein isolate (CPI), respectively. The CPH with the DH of 20% had the highest effect against MDA-231 and T47D cancer cell lines with growth inhibition of 78.2 and 66.2%, which were 6.5 and 6 times higher activities compared to the CPI, respectively. The amino acid profile of CPH indicated that glutamine (15.7%) and asparagine (10.9%) were predominant.


Antioxidant Antiproliferate Protein hydrolysate Cuttlefish Sepia pharaonis 



The authors would like to thank Tarbiat Modares University for the financial support and Prof. Joe M. Regenstein for the editing.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2017

Authors and Affiliations

  • Ali Hamzeh
    • 1
  • Masoud Rezaei
    • 1
    Email author
  • Saber Khodabandeh
    • 2
  • Ali Motamedzadegan
    • 3
  • Mehrdad Noruzinia
    • 4
  1. 1.Department of Seafood ProcessingTarbiat Modares UniversityNoorIran
  2. 2.Department of Marine BiologyTarbiat Modares UniversityNoorIran
  3. 3.Department of Food ScienceAgricultural Sciences and Natural Resources University of SariSariIran
  4. 4.Department of HematologyTarbiat Modares UniversityTehranIran

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