Effect of some preservation techniques on quality parameters and antioxidant properties of banana pulp during storage

  • Rafiya Bazaz
  • Waqas N. Baba
  • Farooq Ahmad Masoodi
  • Sabeeha Yaqoob
Original Paper


Banana is a highly nutritious fruit that can be used for production of various novel foods. Although economical, its limited shelf life hinders the storage of pulp for processing purposes. Chemical (0.1 and 0.1% KMS), thermal (65 °C for 30 min) and a combination of both thermal & chemical treatment was applied to freshly prepared banana pulp and various quality parameters of treated as well as untreated (control) pulp were studied for a storage period of 90 days. All the studied parameters viz; acidity, pH, TSS, reducing and non-reducing sugars varied significantly with different treatments during storage. Color, PPO activity and antioxidant properties (DPPH, ABTS and total phenolic content) were also determined. Chemically treated pulp showed highest values of total phenolic content (0.96–0.44 mg GAE/g dfw) but lowest DPPH (IC50 = 4.42–32.42 mg/ml) and ABTS (IC50 = 3.15–21.37 mg/ml) values. Although lowest values of PPO activity (158.65 units) was seen in samples that were subjected to dual (chemical and thermal) treatment, however lowest change in color index (1.09–58.49) among all the samples during storage was seen in chemically treated samples. Dual treatment was more showed lesser total plate count than samples that were heat or chemically treated samples. All treatments resulted in improved sensory attributes during storage and prolonged the shelf life of samples in comparison to control. Chemical treatment was most effective in preserving quality parameters viz; color and antioxidant activity but samples treated with a combination of thermal and chemical treatment could be stored for longer period without deterioration than rest of the samples.


Banana Physico-chemical properties Antioxidant PPO Preservation 



The authors are thankful to the Department of Biotechnology, Govt. of India for their financial support.

Compliance with ethical standards

Conflict of interest

The authors have no conflict of interest.


  1. 1.
    INIBAP, (International Network for the Improvement OF Banana AND Plantain). The many uses of Musa. (2002)
  2. 2.
    P. Dhar, S. Das, S. Banerjee, S. Mazumder, Production of banana alcohol and utilization of banana residue. Int. J. Res. Eng. Technol. 2, 466–470 (2013)CrossRefGoogle Scholar
  3. 3.
    H.R.P. Fernando, V. Srilaong, N. Pongprasert, P. Boonyaritthongchai, P. Jitareerat, Changes in antioxidant properties and chemical composition during ripening in banana variety ‘Hom Thong’ (AAA group) and ‘Khai’ (AA group). Int. Food Res. J. 21, 749–754 (2014)Google Scholar
  4. 4.
    D.T.P. Darsini, V. Maheshu, M. Vishnapriya, J.M. Sasikumar, In vitro antioxidant activity of banana Musa spp. ABB. CvPisanfakwak. Indian J. Biochem. Biophys. 49, 124–129 (2012)Google Scholar
  5. 5.
    P.K. Agarwal, A. Singh, K. Gaurav, S. Goel, H.D. Khanna, R.K. Goel, Evaluation of wound healing activity of extracts of plantain banana (Musa sapientum var. paradisiaca) in rats. Ind. J. Exp. Biol. 47, 322–340 (2009)Google Scholar
  6. 6.
    R.K. Goel, K. Sairam, Anti-ulcer drugs from indigenous sources with emphasis on Musa sapientum, Tamrabhasma, Asparagus racemosus and Zingiberofficinale. Indian J. Pharmacol. 34, 100–110 (2002)Google Scholar
  7. 7.
    M. Shafi, W.N. Baba, F.A. Masoodi, Composite flour blends: influence of particle size of water chestnut flour on nutraceutical potential and quality of Indian flat breads. Food Measur. 11, 1094–1105 (2016)CrossRefGoogle Scholar
  8. 8.
    S. Tewtrakul, A. Itharat, P. Thammaratwasik, B. Ooraikul, Antiallergic and anti-microbial activities of some Thai crops. Songklanakarin. J. Sci. Technol. 30, 467–473 (2008)Google Scholar
  9. 9.
    C.A. Papaharalambus, K.K. Griendling, Basic mechanisms of oxidative stress and reactive oxygen species in cardiovascular injury. Trends Cardio. Med. 17, 48–54 (2007)CrossRefGoogle Scholar
  10. 10.
    T. Stangeland, S.F. Remberg, K.A. Lye, Total antioxidant activity in 35 Ugandan fruits and vegetables. Food Chem. 113, 85–91 (2009)CrossRefGoogle Scholar
  11. 11.
    D.M. Decosta, H.R. Erabadupitiya, An integrated method to control postharvest diseases of banana using a member of the Burkholderiacepacia complex. Post. Biol. Technol. 36, 31–39 (2005)CrossRefGoogle Scholar
  12. 12.
    J.R. Chiply, Sodium benzoate and benzoic acid, in ed. by A.l. Baren, P.M. Davidson Antimicrobials in Foods. New York (1983). pp. 11–35Google Scholar
  13. 13.
    R. Bazaz, W.N. Baba, F.A. Masoodi, Development and quality evaluation of hypoallergic complementary foods from rice incorporated with sprouted green gram flour. Cogent Food Agric. 2, 1154714 (2016)Google Scholar
  14. 14.
    R. Bazaz, W.N. Baba, F.A. Masoodi, S. Yaqoob, Formulation and characterization of hypo allergic weaning foods containing potato and sprouted green gram. Food Measur. 10, 453–465 (2016)CrossRefGoogle Scholar
  15. 15.
    W.N. Baba, S. Din, H.A. Punoo, T.A. Wani, M. Ahmad, F.A. Masoodi, Comparison of cheese and paneer whey for production of a functional pineapple beverage: nutraceutical properties and shelf life. J. Food Sci. Technol. 53, 2558–2568 (2016)CrossRefGoogle Scholar
  16. 16.
    S. Rangana, Hand Book of Analysis and Quality Control for Fruit and Vegetable Products. Tata McGraw-Hill Publishing Company Ltd., New Delhi. (1991) p. 1112Google Scholar
  17. 17.
    J. Ciavano, Whiteness, yellowness, and browning in food colorimetry. J.L. Caivano, M.P. Buera, Color in Food: Technological and Psychophysical Aspects (CRC Press, Boca Raton, 2012), pp. 93–104Google Scholar
  18. 18.
    AACC In Approved Methods of American Association of Cereal Chemists, Method 10-50D, 10th edn. (American Association of Cereal Chemists, St. Paul, 2000)Google Scholar
  19. 19.
    M. Ahmad, W.N. Baba, A. Gani, T.A. Wani, A. Gani, F.A. Masoodi, Effect of extraction time on antioxidants and bioactive volatile components of green tea (Camellia sinensis), using GC/MS. Cogent Food Agric. 1, 1–11 (2015)Google Scholar
  20. 20.
    M. Shafi, W.N. Baba, F.A. Masoodi, R. Bazaz, Wheat water chestnut flour blends: effect of baking on antioxidant properties of cookies. J. Food Sci. Technol. 53, 4278–4288 (2016)CrossRefGoogle Scholar
  21. 21.
    R. Baskar, B. Shrisakthisathyapriya, R. Shyampriya, R. Nithya, P. Poongodi, Antioxidant potential of peel extracts of banana varieties (Musa sapientum). Food Nutr. Sci. 2, 1128–1133 (2011)CrossRefGoogle Scholar
  22. 22.
    S. Muzzaffar, W.N. Baba, N. Nazir, F.A. Masoodi, M.M. Munaff, R. Bazaz, Effect of storage on physicochemical, microbial and antioxidant properties of pumpkin (Cucurbita moschata) candy. Cogent Food Agric. 2, 1–12 (2016)Google Scholar
  23. 23.
    M.Z. Hossain, M.K. Hassan, G.N. Hasan, M.R. Islam, Effects of modified atmosphere packaging and low temperatures on the physico-chemical changes and shelf life of banana. Acad. J. Plant Sci. 6, 19–31 (2013)Google Scholar
  24. 24.
    A. Zewter, K. Woldetsadik, T.S. Workneh, Effect of 1-methylcyclopropene, potassium permanganate and packaging on quality of banana. Afr. J. Agri. Res. 7, 2425–2437 (2012)Google Scholar
  25. 25.
    R. Yadav, A.D. Tripathi, A. Jha, Effect of storage time on the physicochemical properties and sensory attributes of Aloe vera ready-to-serve (RTS) beverage. Int. J. Food Nutri. Public Health 6, 173–192 (2013)Google Scholar
  26. 26.
    Y. Durrani, M. Ayub, A. Muhammad, A. Ali, Physicochemical response of apple pulp to chemical preservatives and antioxidant during storage. Int. J. Food Saf. 12, 20–28 (2010)Google Scholar
  27. 27.
    M.I.F. Chitarra, A.B. Chitarra, Pós-colheita de frutas e hortaliças: fisiologia e manuseio. 3122 (UFLA, Lavras, 2005), p. 785Google Scholar
  28. 28.
    I. Hussain, A. Zeb, I. Shakir, A.S. Shah, Combined effect of potassium sorbate and sodium benzoate on individual and blended juices of apricot and apple fruits grown in Azad Jammu and Kashmir. Pak. J. Nutr. 7, 181–185 (2008)CrossRefGoogle Scholar
  29. 29.
    L. Li, X. Li, A. Wang, Y. Jiang, Z. Ban, Effect of heat treatment on physicochemical, color, antioxidant and micro structural characteristics of apples during storage. Int. J. Food Sci. Technol. 48, 727–734 (2012)CrossRefGoogle Scholar
  30. 30.
    S.L.S. Bico, M.F.J. Raposo, R.M.S.C. Morais, A.M.M.B. Morais, Combined effects of chemical dip and/or carrageenan coating and/or controlled atmosphere on quality of fresh-cut banana. Food Control 20, 508–514 (2009)CrossRefGoogle Scholar
  31. 31.
    A. Piga, I. Pinna, K.B. Ozer, M. Agabbio, U. Aksoy, Hot air dehydration of figs (Ficus carica L.): drying kinetics and quality loss. Int. J. Food Sci. Technol. 39, 793–799 (2004)CrossRefGoogle Scholar
  32. 32.
    M.A. Madrau, A. Piscopo, A.M. Sanguinetti, A. Del Caro, M. Poiana, F.V. Romeo, A. Piga, Effect of drying temperature on polyphenolic content and antioxidant activity of apricots. Eur. Food Res. Technol. 228, 441–448 (2009)CrossRefGoogle Scholar
  33. 33.
    D.S. Kumhar, S. Pareek, K.D. Ameta, Effect of antioxidants and storage temperatures on browning and quality of custard apple (Annona squamosal L.) pulp. J. Sci. Ind. Res. 73, 622–626 (2014)Google Scholar
  34. 34.
    W.H. Shah, N.A. Sufi, S.I. Zafar, Studies on the storage stability of guava fruit juice. Pak. J. Sci. Ind. Res. 18, 179–183 (1975)Google Scholar
  35. 35.
    A.S. Rathod, B.A. Shakya, K.D. Ade, Studies on effect of thermal processing on preparation of bael fruit RTS blended with Aonla. Int. J.Res. Eng. Adv. Technol. 2, 1–6 (2014)Google Scholar
  36. 36.
    A. Salvador, T. Sanz, S.M. Fiszman, Changes in colour and texture and their relationship with eating quality during storage of two different dessert bananas. Postharvest Biol. Technol. 43, 319–325 (2007)CrossRefGoogle Scholar
  37. 37.
    C. Benhura, T. Rukuni, C. Kadema, B. Mubvakure, R. Nazare, P.E. Gombiro, B. Tokwe, E. Matangi, A. Madzima, Preservation of mango pulp of fruit from Rusitu Valley, Chimanimani in Zimbabwe. Pak. J. Food Sci. 22, 191–196 (2012)Google Scholar
  38. 38.
    N. Kuashik, B.P. Kaur, P.S. Roa, H.N. Mishra, Effect of high pressure processing on color, biochemical and microbiological characteristics of mango pulp (Mangifera indica cv. Amrapali). Innov. Food Sci. Emerg. Technol. 22, 40–50 (2014)CrossRefGoogle Scholar
  39. 39.
    M. Abd-Elhady, Effect of citric acid, calcium lactate and low temperature prefreeze treatment on the quality of frozen strawberry. Ann. Agri. Sci. 59, 69–75 (2014)Google Scholar
  40. 40.
    D. Sirohi, S. Patel, P.L. Choudhary, C. Sahu, Studies on preparation and storage of whey-based mango herbal Pudina (Mentha arvensis) beverage. J Food Sci. Technol. 42, 157–161 (2005)Google Scholar
  41. 41.
    M.S. Ali, H. Raza, M.A. Khan, M. Hussain, Effect of different periods of ambient storage on chemical composition of apple fruit. Int. J. Agri. Biol. 6, 568–571 (2004)Google Scholar
  42. 42.
    M. Gharezi, N. Joshi, E. Sadeghian, Effect of post-harvest treatment on stored cherry tomatoes. J. Nutr. Food Sci. 2, 157–167 (2012)CrossRefGoogle Scholar
  43. 43.
    K.A. Hakim, M.A.R. Sarkar, M.Z.H. Khan, S.M. Rahman, M. Ibrahim, M.K. Islam, Effect of post-harvest treatments on physiochemical characters during storage of two banana (Musa spp. L.) cv. Sabri and Amritsagar. Int. J. Bios. 3, 168–179 (2013)Google Scholar
  44. 44.
    V.S. Barwal, S.K. Shrera, Standardization of extraction methods and preservation techniques of hill lemon juice. J. Sci. Ind. Res. 68, 608–610 (2009)Google Scholar
  45. 45.
    Hameed, Storage stability of carrot pulp at various condition and treatment. Indian Food Packer 45, 48–53 (1996)Google Scholar
  46. 46.
    A.N. Chowdhury, A. Nargis, M.Z. Rahman, A.K.M.S. Alam, M. Ibrahim, S. Akhter, Freezing adaptability and chemical composition of Strawberry (Fragaria Xananassa Duch.) in Bangladesh. IOSR JESTFT 7, 50–54 (2013)CrossRefGoogle Scholar
  47. 47.
    K.S. Wickramarachchi, S.L. Ranamukhaarachchi, Preservation of fiber-rich banana blossom as a dehydrated vegetable. Sci. Asia 31, 265–271 (2005)CrossRefGoogle Scholar
  48. 48.
    H.A. Eissa, M.M. Hassanane, H.A. Sharaf, Effect of colorant on cytogenetic, biochemical and histochemical parameters and quality changes during storage of some commercial fruit drinks. J. Nutr. Food Sci. 4, 1–11 (2014)CrossRefGoogle Scholar
  49. 49.
    A. Ibarz, J. Pagan, S. Garza, Kinetic models of non-enzymatic browning in apple puree. J. Sci. Food Agri. 80, 1162–1168 (2000)CrossRefGoogle Scholar
  50. 50.
    R. Nisar, W.N. Baba, F.A. Masoodi, Effect of chemical and thermal treatments on quality parameters and antioxidant activity of apple (pulp) grown in high Himalayan regions. Cogent Food Agric. 1, 1–13 (2015)Google Scholar
  51. 51.
    A. Landl, M. Abadias, C. Sarraga, I. Vinas, P.A. Picouet, Effect of high pressure processing on the quality of acidified Granny Smith apple puree product. Innovative Food Sci. Emerg. Technol. 11, 557–564 (2010)CrossRefGoogle Scholar
  52. 52.
    S.F. Aguilar-Rosas, M.L. Ballinas-Casarrubias, G.V. Nevarez-Moorillon, O. Martin-Belloso, E. Ortega-Rivas, Thermal and pulsed electric fields pasteurization of apple juice: Effects on physicochemical properties and flavor compounds. J. Food Eng. 83, 41–46 (2007)CrossRefGoogle Scholar
  53. 53.
    T.B.T. Nguyen, S. Ketsa, W.G. VanDoorn, Relationship between browning and the activities of polyphenol oxidase and phenylalanine ammonia lyase in banana peel during low temperature storage. Postharvest Biol. Technol. 30, 187–193 (2003)CrossRefGoogle Scholar
  54. 54.
    M. Pilar-Cano, B. Ancos, M.G. Lobo, Improvement of frozen banana (Musa cavendishii, cv. Enana) color by blanching: relationship between browning, phenols and polyphenol oxidase and peroxidase activities. Lebensmittel Unters Forsch A 204, 60–65 (1997)CrossRefGoogle Scholar
  55. 55.
    Y. Yu, G. Xiao, J.X.U.Y. Wu, D. Tang, Y. Chen, J. Wen, M. Fu, Comparing characteristic of banana juices from banana pulp treated by high pressure carbon dioxide and mild heat. Inn. Food Sci. Emerg. Technol. 18, 95–100 (2013)CrossRefGoogle Scholar
  56. 56.
    H. Huang, Q. Zhu, Z. Zhang, B. Yang, X. Duan, Y. Jiang, Effect of oxalic acid on antibrowning of banana (Musa spp., AAA group, cv. ‘Brazil’) fruit during storage. Sci. Hort. 160, 208–212 (2013)CrossRefGoogle Scholar
  57. 57.
    K. Saengnil, K. Lueangprasert, J. Uthaibutra, Control of enzymatic browning of harvested ‘Hong Huay’ Litchi fruit with hot water and oxalic acid dips. Sci. Asia 32, 345–350 (2006)CrossRefGoogle Scholar
  58. 58.
    A. Altunkaya, V. Gokmen, Purification and characterization of polyphenol oxidase, peroxidase and lipoxygenase from freshly cut lettuce. Food Technol. Biotechnol. 49, 249–256 (2011)Google Scholar
  59. 59.
    Y.M. Jiang, L. Pen, J. Li, Use of citric acid for shelf life and quality maintenance of fresh-cut Chinese water chestnut. J. Food Eng. 63, 325–328 (2004)CrossRefGoogle Scholar
  60. 60.
    M. Ihl, L. Aravena, E. Scheuermann, E. Uquiche, V. Bifani, Effect of immersion solutions on shelf life of minimally processed lettuce. Lebensmittel-Wissenschaft und-Technol. 36, 591–599 (2003)CrossRefGoogle Scholar
  61. 61.
    R. Lo Scalzo, M. Fibiani, G. Mennella, L. Giuseppe, G.L. Rotino, M.D. Sasso, M. Culici, A. Spallino, P.C. Braga, Thermal treatment of eggplant (Solanum melongena L.) increases the antioxidant content and the inhibitory effect on human neutrophil burst. J. Agric. Food Chem. 58, 3371–3379 (2010)CrossRefGoogle Scholar
  62. 62.
    G. Budryn, E. Nebesny, D. Rosiak-Rachwal, Stability of hydroxycinnamic acids and caffeine from green coffee extracts after heating in food model systems. Eur. Food Res. Technol. 236, 969–978 (2013)CrossRefGoogle Scholar
  63. 63.
    I. Klimczak, M. Matecka, M. Szlachta, A. Gliszczynska-Swiglo, Effect of storage on the content of polyphenols, vitamin C and the antioxidant activity of orange juices. J. Food Compos. Anal. 20, 313–322 (2007)CrossRefGoogle Scholar
  64. 64.
    P.H. Hsieh, T.F. Lien, Study of the physico-chemical properties an antioxidant activity of extracted melanins. J. Agri. Sci. 4, 217–229 (2012)Google Scholar
  65. 65.
    M.R. Manikantan, R. Sharma, R. Kasturi, N. Varadharju, Storage stability of banana chips in polypropylene nanocomposite packaging films. J. Food Sci. Technol. 51, 2990–3001 (2014)CrossRefGoogle Scholar
  66. 66.
    S. Akhtar, M. Riaz, A. Ahmad, A. Nisar, Physico-chemical, microbiological and sensory stability of chemically preserved mango pulp. Pak. J. Bot. 42, 853–862 (2010)Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Rafiya Bazaz
    • 1
  • Waqas N. Baba
    • 2
  • Farooq Ahmad Masoodi
    • 2
  • Sabeeha Yaqoob
    • 1
  1. 1.Institute of Home ScienceUniversity of KashmirSrinagarIndia
  2. 2.Department of Food Science and TechnologyUniversity of KashmirSrinagarIndia

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