Optimization and analysis of microwave-assisted extraction of bioactive compounds from Mimosa pudica L. using RSM & ANFIS modeling

  • Vinothapooshan Ganesan
  • Vijaya Gurumani
  • Selvaraj Kunjiappan
  • Theivendran Panneerselvam
  • Balasubramanian Somasundaram
  • Suthendran Kannan
  • Anindita Chowdhury
  • Govindraju Saravanan
  • Chiranjib Bhattacharjee
Original Paper


The central composite rotatable design (CCRD) based response surface methodology (RSM) and adaptive neuro-fuzzy inference system (ANFIS) statistical methodology was used to design and identify highly efficient extraction process parameters to get high yield of bioactive compound from Mimosa pudica L. In general, many of the process parameters are need to be effectively involved to maximize the yield of bioactive compounds. In this relation, the independent process parameters such as methanol concentration (X 1), microwave power (X 2), irradiation temperature (X 3) and irradiation time (X 4) was chosen in the process of microwave assisted extraction (MAE). The observed studied parametes were produced effective results in the range of 60–85% methanol concentration, 15–25% microwave power, 40–60 °C irradiation temperature and irradiation time 10–15 min. Moreover the optimal yields of TPC and TFC are 635–640 mg gallic acid equivalents (GAE)/g and 61.53–61.76 mg rutin equivalents (RU)/g of extract, and their antioxidant activities are 68.7–72.6% DPPHsc, 76.1–76.86% ABTSsc and FRAB value of 65.24–66.94 µg mol (Fe (II))/g could be obtained for specific optimized process variables. Further, the bioactive compound mimopudine was identified through high performance liquid chromatography (HPLC) in the obtained extract.


Mimosa pudica L. RSM ANFIS CCRD Bioactive compounds Mimopudine 



We gratefully thank the Chancellor, Vice-chancellor and Directors of Kalasalingam University, Krishnankoil, India for research fellowships and utilizing research facilities. We thank Prof. Z. Maciej Gliwicz, Ms. Ewa Babkiwics, Dr. Piotr Maszczyk, Department of Hydrobiology, Faculty of Biology, University of Warsaw, Warszawa, Poland, for their prompt support and suggestions.


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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Vinothapooshan Ganesan
    • 1
  • Vijaya Gurumani
    • 2
  • Selvaraj Kunjiappan
    • 3
  • Theivendran Panneerselvam
    • 4
  • Balasubramanian Somasundaram
    • 3
  • Suthendran Kannan
    • 5
  • Anindita Chowdhury
    • 6
  • Govindraju Saravanan
    • 7
  • Chiranjib Bhattacharjee
    • 8
  1. 1.Department of PharmaceuticsArulmigu Kalasalingam College of PharmacyVirudhunagarIndia
  2. 2.Department of Computer Science and EngineeringKalasalingam Institute of TechnologyVirudhunagarIndia
  3. 3.Sir CV Raman-KS Krishnan International Research CentreKalasalingam UniversityVirudhunagarIndia
  4. 4.Department of Pharmaceutical ChemistryKaravali College of PharmacyMangaloreIndia
  5. 5.National Cyber Defence Research CentreKalasalingam UniversityVirudhunagarIndia
  6. 6.Department of BiotechnologyKalasalingam UniversityVirudhunagarIndia
  7. 7.MNR College of PharmacyTelanganaIndia
  8. 8.Department of Chemical EngineeringJadavpur UniversityKolkataIndia

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