Abstract
The control of pathogenic bacteria and oxidative factors is important to keep microbiological and physicochemical traits of meat products. In this study, cold-pressed black cumin seed oil (BCSO) was incorporated (at levels of 1, 2, and 4%, w/w) into ground beef meat (GBM) to test the antimicrobial activity of BCSO on the growth inhibition of food-borne pathogens (Listeria monocytogenes Scott A and Salmonella Enteritidis PT4) artificially inoculated (~4.5 log CFU/g) in air-packed GBM during storage at 4 °C for 15 days. BCSO was analyzed for fatty acids, sterols, tocopherols, and thymoquinone using gas chromatography and high-performance liquid chromatography. BCSO induced higher inhibition zone (15–17 mm) against both pathogenic bacteria as compared with the control. The BCSO supplementation retarded the total bacterial count, while the food-borne pathogens were unable to grow in BCSO-supplemented GBM during cold storage for 15 days. After 15 days, S. Enteritidis PT4 was more resistant than L. monocytogenes Scott A in GBM supplemented with 4% BCSO. In general, the shelf life of BCSO-supplemented GBM was extended under refrigerated conditions with low microbial loads. BCSO-supplemented GBM also improved oxidative stability and sodium dodecyl sulfate polyacrylamide gel electrophoresis profile.
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J.C. Buzby, T. Roberts, C.-T.J. Lin, J. M. Macdonald, Bacterial foodborne disease: Medical costs and productivity losses. Economic Research Service/USDA. AER-741 (1996)
M. Luther, J. Parry, J. Moore, J. Meng, Y. Zhang, Z. Cheng, L. Yu, Food Chem. 104, 1065–1073 (2007)
N.S. Alzoreky, K. Nakahara, Int. J Food Microbiol. 80, 223–230 (2003)
B. Shan, Y.-Z. Cai, J.D. Brooks, H. Corke, Int. J Food Microbiol. 117, 112–119 (2007)
M. Viuda-Martos, M.A. Mohamady, J. Fernández-Lópeza, K.A. Abd ElRazik, E.A. Omer, J.A. Pérez-Alvarez, E. Sendra, Food Control 22, 1715–1722 (2011).
S. Mahgoub, A. Osman, M. Sitohy, J. Food Prot. 74, 1475–1481 (2011)
M.Z. Sitohy, S.A. Mahgoub, A.O. Osman, Int. J Food Microbiol. 154, 19–29 (2012)
M.H.M. Abdeldaiem, H.G.M. Ali, M.F. Ramadan, Food Meas. (2017). doi:10.1007/s11694-017-9520-7
M.F. Ramadan, R.M. Elsanhoty, Food Chem. 133, 1169–1176 (2012)
S. Mahgoub, M. Sitohy, A. Osman, Food Bioprocess Technol. 6, 101–109 (2013)
M.F.R. Hassanien, S.A. Mahgoub, K.M. El-Zahar, Saudi J. Biol. Sci. 21, 280–288 (2014)
T.N. Noumo, P.D. Mbougueng, L.N. Tatsadjieu, A.T. Sokamte, C.M.F. Mbofung, Food Meas. 10, 480–492 (2016)
P. Pereira, B. Huerta, C. Borge, R. Astorga, R. Romero, A. Perea, Acta Pathol. Microbiol. Immunol. Scand. 113, 1–6 (2014)
A.H. EL-Ghorab, M. Nauman, F.M. Anjum, S. Hussin, M. Nadeem, J. Agric. Food Chem. 58, 8231–8237 (2010)
S.A. Mahgoub, M.F. Ramadan, K.M. El-Zahar, J. Food Saf. 33, 470–480 (2013)
M.F. Ramadan, Int. J. Food Sci. Technol. 42, 1208–1218 (2007)
H. Lutterodt, M. Luther, M. Slavin, J.-J. Yin, J. Parry, J.-M. Gao, L. Yu, LWT-Food Sci. Technol. 43, 1409–1413 (2010)
J. Parry, L. Su, J. Moore, Z. Cheng, M. Luther, J.N. Rao, J.-Y. Wang, L. Yu, J. Agric. Food Chem. 54, 3773–3778 (2006)
M.F. Ramadan, Ind. Crops Prod. 43, 65–72 (2013)
J. Ahn, I.U. Grun, A. Mustapha, J. Food Prot. 67, 148–155 (2004)
M.F. Ramadan, M.S. Asker, M. Tadros, Eur. Food Res. Technol. 234, 833–844 (2012)
M. Kiralan, G. Özkan, A. Bayrak, M. F. Ramadan, Ind. Crops Prod. 57, 52–58 (2014).
K.A. Hammer, C.F. Carson, T.V. Riley, J. Appl. Microbiol. 86, 985–990 (1999)
Y. Pranoto, V.M. Salokhe, S.K. Rakshit, Food Res Int 38, 267–272 (2005)
AOAC, in Official Methods of Analysis, 17th edn. (Association of Official Analytical Chemists, International, Inc., Arlington, 2002)
S. Jung, J. Choe, B. Kim, H. Yun, Z.A. Kruk, C. Jo, Meat Sci. 86, 520–526 (2010)
M.S. Blois, Nature 181, 1199–1200 (1958)
W.G. Niehius, B. Samuelson, Eur. J. Biochem. 6, 126–130 (1968)
U.K. Laemmli, Nature 227, 680–685 (1970)
International Organization for Standardization, Microbiology-General Guidance on Methods for the Detection of Salmonella, 2nd edn., rev. ISO 6579. (International Organization for Standardization, Geneva, 1991)
International Organization for Standardization, Microbiology of food and animal feeding stuffs. Horizontal methods for the detection and enumeration of Listeria monocytogenes. Part 2: enumeration method. ISO 11290. (International Organization for Standardization, Geneva, 1998)
T.D. Parker, D.A. Adams, K. Zhou, M. Harris, L. Yu, J. Food Sci. 68, 1240–1243 (2003)
R. Schneider-Stock, I.H. Fakhoury, A.M. Zaki, C.O. El-Baba, H.U. Gali-Muhtasib, Drug Discov. Today 9, 18–30 (2014)
S.O. Agunbiade, O.A. Akintobi, O.M. Ighodaro, Life Sci. J. 7, 47–51 (2010)
D.D. Pawar, S.V.S. Malik, K.N. Bhilegaonkar, S.B. Barbuddhe, Meat Sci. 56, 215–219 (2000)
A. Philanto, Int. Dairy J. 16, 1306–1314 (2006)
K.L. Parkin, S. Damodaran, in Encyclopedia Food Science and Nutrition (Academic Press, San Diego, 2003), pp. 4288–4294
I. Bettaieb, S. Bourgou, W.A. Wannes, I. Hamrouni, F. Limam, B. Marzouk, J. Agric. Food Chem. 58, 10410–10418 (2010)
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Mahgoub, S.A.M., Osman, A. & Ramadan, M.F. Inhibitory effect of Nigella sativa oil against Listeria monocytogenes and Salmonella Enteritidis inoculated in minced beef meat. Food Measure 11, 2043–2051 (2017). https://doi.org/10.1007/s11694-017-9587-1
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DOI: https://doi.org/10.1007/s11694-017-9587-1