Abstract
Electrical impedance spectroscopy (EIS) as non-destructive investigation has been conducted to study the electrical impedance variations during ripening of mandarin orange. The objective of the work is to study the electrical impedance variations and variations in weight of the orange fruit with different ripening state. Electrical equivalent circuit has been modeled relative to the Nyquist plot obtained during the ripening of orange by non-linear curve fitting technique. EIS studies on orange fruit have been conducted by applying a small amount of alternating current through an array of Ag/AgCl electrodes attached to the orange fruit. The impedance and phase angles of orange fruit are measured at frequency sweep from 50 Hz to 1 MHz for 100 frequency points. The results revealed that the impedance, real part and imaginary part of the impedance all are increased and the weight of orange are decreased with the increase in ripening state. It is observed that the electrical equivalent circuit of orange fruit contains a constant phase element.
Similar content being viewed by others
References
J.R. Macdonald, Impedance Spectroscopy. Ann. Biomed. Eng. 20, 289–305 (1992)
J.R. Macdonald, W.B. Johnson, (2005). Fundamentals of impedance spectroscopy. Impedance spectroscopy: theory, experiment, and applications, Second Edition, 1–26
A. Lasia, Electrochemical impedance spectroscopy and its applications. In Modern Aspects of Electrochemistry, (Springer, New York, 2002), pp. 143–248
B.Y. Chang, S.M. Park, Electrochemical impedance spectroscopy. Annu. Rev. Anal. Chem. 3, 207–229 (2010)
T.K. Bera, J. Nagaraju, Electrical impedance spectroscopic study of broiler chicken tissues suitable for the development of practical phantoms in multifrequency EIT. J. Electr. Bioimpedance 2, 48–63 (2011). doi:10.5617/jeb.174
T.K. Bera, Y. Mohamadou, K.H. Lee, H. Wi, T.I. Oh, E.J. Woo, M. Soleimani, J.K. Seo, Electrical impedance spectroscopy for electro-mechanical characterization of conductive fabrics. Sensors 14, 9738–9754 (2014)
T.K. Bera, Bioelectrical impedance methods for noninvasive health monitoring: a review. J. Med. Eng. 2014(381251), 28 (2014). doi:10.1155/2014/381251
J. Santos, F.M. Janeiro, M.P. Ramos, Impedance frequency response measurements with multiharmonic stimulus and estimation algorithms in embedded systems. Measurement 48, 173–182 (2014)
Patrick O. Moore, Michael W. Allgaier and Robert E. Cameron, Nondestructive Testing Handbook, Third Edition: 9, Visual Testing, The American Society for Non-destructive Testing, 2010
Bera, T. K., Nagaraju, J., & Lubineau, G. Electrical impedance spectroscopy (EIS)-based evaluation of biological tissue phantoms to study multifrequency electrical impedance tomography (Mf-EIT) systems. J Visualization 1–23. 2016.
A. Keshtkar, Virtual bladder biopsy using bio-impedance spectroscopy at 62.500 Hz–1.024 MHz. Measurement 40(6), 585–590 (2007)
P. Arpaia, F. Clemente, C. Romanucci, An instrument for prosthesis osseointegration assessment by electrochemical impedance spectrum measurement. Measurement 41(9), 1040–1044 (2008)
P.M. Ramos, F.M. Janeiro, Gene expression programming for automatic circuit model identification in impedance spectroscopy: performance evaluation. Measurement 46(10), 4379–4387 (2013)
B. Sanchez, E. Louarroudi, R. Pintelon, Time–frequency analysis of time-varying in vivo myocardial impedance. Measurement 56, 19–29 (2014)
M. Min, O. Märtens, T. Parve, Lock-in measurement of bio-impedance variations. Measurement 27(1), 21–28 (2000)
D. Bouchaala, O. Kanoun, N. Derbel, High accurate and wideband current excitation for bioimpedance health monitoring systems. Measurement 79, 339–348 (2015)
M. Grossi, M. Lanzoni, R. Lazzarini, B. Riccò, Automatic ice-cream characterization by impedance measurements for optimal machine setting. Measurement 45(7), 1747–1754 (2012)
J.M. Cruza, I.C. Fitaa, L. Sorianob, J. Payáb, M.V. Borracherob, The use of electrical impedance spectroscopy for monitoring the hydration products of Portland cement mortars with high percentage of pozzolans. Cement Concr. Res. 50, 51–61 (2013)
S.L. Zelinka, D.R. Rammer, D.S. Stone, Impedance spectroscopy and circuit modeling of Southern pine above 20% moisture content. Holzforschung 62, 737–744 (2008) Copyright © by Walter de Gruyter, Berlin, New York. doi:10.1515/HF.2008.115
J. Hoja, G. Lentka, An analysis of a measurement probe for a high impedance spectroscopy analyzer. Measurement 41(1), 65–75 (2008)
J. Huang, Z. Xu, S. Zhao, S. Li, X. Feng, P. Wang, Z. Zhang, Study on carrier mobility measurement using electroluminescence in frequency domain and electrochemical impedance spectroscopy. Measurement 43(3), 295–298 (2010)
P.M. Gomadam, J.W. Weidnern, Analysis of electrochemical impedance spectroscopy in proton exchange membrane fuel cells. Int. J. Energy Res. 29, 1133–1151 (2005)
D.A. Dean, T. Ramanathan, D. Machado, R. Sundararajan, Electrical Impedance Spectroscopy Study of Biological Tissues. J. Electrostat. 66(3–4), 165–177 (2008)
P. Héroux, M. Bourdages, Monitoring living tissues by electrical impedance spectroscopy. Ann. Biomed. Eng. 22(3), 328–337 (1994)
A. Chowdhury, T.K. Bera, D. Ghoshal, B. Chakraborty, Studying the electrical impedance variations in banana ripening using electrical impedance spectroscopy (EIS). In Computer, Communication, Control and Information Technology (C3IT), 2015 Third International Conference on (pp. 1–4). IEEE (2015)
F.R. Harker, J.H. Maindonald, Ripening of Nectarine Fruit. Plant Physiol. 106, 165–171 (1994)
F.R. Harker, J. Dunlop, Electrical impedance studies of nectraines during coolstage and fruit ripening. Postharvest Biol. Technol. 4, 125–134 (1994)
F.Roger Harker, ShelleyK. Forbes, ‘Ripening and development of chilling injury in persimmon fruit’, New Zealand. J. Crop Hortic. Sci. 25, 149–157 (1997)
P.J. Jackson, F.R. Harker, Apple bruise detection by electrical impedance measurement. HortScience 35(1), 104–107 (2000)
P. Mészáros, Relationships between electrical parameters and physical properties of cereal grains, oilseeds, and apples. Budapest. (2007)
A.D. Bauchot, F.R. Harker, W.M. Arnold, The use of electrical impedance spectroscopy to assess the physiological condition of kiwifruit. Postharvest Biol. Technol. 18, 9–18 (2000)
R.C. Bean, J.P. Rasor, G.G. Porter, Changes in electrical characteristics of avocados during ripening. California Avocado Soc. Yearbook 44, 75–78 (1960)
J. Juansah, I.W. Budhiastra, K. Dahlan, K.B. Seminnar, Electrical behavior of garut citrus fruits during ripening changes in resistance and capacitance models of internal fruits. IJET-IJENS 12(04), 1–8 (2012)
J. Juansah, I.W. Budhiastra, K. Dahlan, K.B. Seminnar, The prospect of electrical impedance spectroscopy as Non-destructive Evaluation of Citrus Fruits acidity. IJETAE 2(11), (2012)
E. Vozáry, P. Benkő, Non-destructive determination of impedance spectrum of fruit flesh under the skin. J. Phys. 224, 012142 (2010)
M. Rehman, B.A.J.A. Abu Izneid, M.Z. Abdullah, M.R. Arshad, Assessment of quality of fruits using impedance spectroscopy. Int. J. Food Sci. Technol. 46, 1303–1309 (2011)
R. Raj, N. Binoy C, Bio impedance spectroscopy for the assessment of quality of fruits by constructing the equivalent circuit. Int. J. Eng. Res. Technol. (IJERT) 2(11), (2013) ISSN: 2278-0181
X. Liu, Electrical Impedance Spectroscopy Applied in Plant, Physiology Studies, in School of Electrical and Computer Engineering (RMIT University, Melbourne, 2006), p. 102
S. Zheng, An Investigation on Electrical Properties of Major Constituents of Grape Must under Fermentation Using Electrical Impedance Spectroscopy’ RMIT University August, 2009
A.R. Varlan, W. Sansen,”Nondestructive electrical impedance analysis in fruit: normal ripening and injuries characterization”. Electro-Magnetobiology 15, 213–227 (1996)
R.I. Hayden, C.A. Moyse, F.W. Calder, D.P. Crawford, D.S. Fensom, Electrical impedance studies on potato and alfalfa tissue. J. Exp. Bot. 20(63), 177–200 (1969)
M.I.N. Zhang, J.H.M. Willison, Electrical impedance analysis in plant tissues: a double shell model. J. Exp. Bot. 42, 1465–1475 (1991)
X. Liu, Q. Fang, S. Zheng, I. Cosic, P. Cao, Electrical impedance spectroscopy investigation on Cucumber Dehydration’ International Society for Horticulture Science Acta Horticulture 804: Europe-Asia Symposium on Quality Management in Postharvest Systems—Eurasia (2007)
B.M.H. Larson, C. Spencer, H. Barrett, A comparative analysis of pollen limitation in flowering plants. Biol. J. Linnean Soc. 69, 503–520 (2000)
Fresh Fruits and Vegetables Manual, A Report from The U.S. Department of Agriculture (USDA), ependence Avenue, SW., Washington, DC 20250-9410, Second Edition Issued 2012
C.J. Brady, Fruit Ripening. Annu. Rev. Plant Physiol. 38, 155–178 (1987)
N.A.M. Eskin (ed.), Quality and Preservation of Fruits (CRC Press, Boca Raton, FL, 1991), p. 212PP
J. Gross, Pigments in Fruits (Academic Press, Inc., Orlando, FL, 1987), p. 303PP
Fruit & Vegetable Nutrition Facts Chart, © 2004 Dole 5 A Expt-Day Program/Dole Food Company, Inc
Fruits, Vegetables, and Health: a Scientific overview, 2011
F.H. Netter, Atlas of Human Anatomy (Rittenhouse Book Distributors. Inc, Philadelphia, 1997)
M.C. Martí, D. Camejo, F. Vallejo, F. Romojaro, S. Bacarizo, J.M. Palma, Jiménez, A, (2011). Influence of fruit ripening stage and harvest period on the antioxidant content of sweet pepper cultivars. Plant foods for human nutrition, 66(4), 416–423
J.J. Ackmann, Complex bioelectric impedance measurement system for the frequency range from 5 Hz to 1 MHz. Ann. Biomed. Eng. 21, 135–146 (1993)
J.J. Ackmann, M.A. Seitz, Methods of complex impedance measurements in biologic tissue. Crit. Rev. Biomed. Eng. 11(4), 281–311 (1984)
K. Cha, G.M. Chertow, J. Gonzalez, J.M. Lazarus, D.W. Wilmore, Multifrequency bioelectrical impedance estimates the distribution of body water. J. Appl. Physiol. 79, 1316–1319 (1995)
H.P. Schwann, Electrical properties of tissue and cell suspensions: mechanisms and models. Proc. IEEE Adv. Biol. Med. Soc. 1, A70–A71 (2002)
S.M.M. Islam, M.A.R. Reza, M.A. Kiber, Development of multi-frequency electrical impedance spectroscopy (EIS) system for early detection of breast cancer. Int. J. Electron. Inform. 2(1), 26–32 (2013)
S. Ouitrakul, M. Sriyudthsak, S. Charojrochkul, T. Kakizono, Impedance analysis of bio-fuel cell electrodes. Biosens. Bioelectron. 23, 721–727 (2007)
J.M. Ruiz, Sensor-Based Garments that Enable the Use of Bioimpedance Technology:Towards Personalized Healthcare Monitoring. Doctoral Thesis, Stockholm, Sweden, 2013
B.K. Van Kreel, N. Cox-Reyven, P. Soeters, Determination of total body water by multifrequency bioelectric impedance: development of several models. Med. Biol. Eng. Comput. 36, 337–345 (1998)
M. Ladanyia, M. Ladaniya, Citrus Fruit: Biology, Technology and Evaluation (Academic press, London, 2010)
S. Nagy, Vitamin C contents of citrus fruit and their products: a review. J. Agric. Food. Chem. 28(1), 8–18 (1980)
C. Economos, W.D. Clay, Nutritional and health benefits of citrus fruits. Energy (kcal) 62(78), 37 (1999)
E.A. Baldwin, (1993). Citrus Fruit. In Biochemistry of Fruit Ripening (pp. 107–149). Springer Netherlands
S.K. Lee, A.A. Kader, Preharvest and postharvest factors influencing vitamin C content of horticultural crops. Postharvest biology and technology 20(3), 207–220 (2000)
D. Ramful, E. Tarnus, O.I. Aruoma, E. Bourdon, T. Bahorun, Polyphenol composition, vitamin C content and antioxidant capacity of Mauritian citrus fruit pulps. Food Res. Int. 44(7), 2088–2099 (2011)
S.S. Hassan, M.A. El Fattah, M.T.M. Zaki,, (1975). Spectrophotometric determination of vitamin C in citrus fruits using peri-naphthindan-2, 3, 4-trione. Fresenius’ Zeitschrift für analytische Chemie, 277(5), 369–371
J. Silalahi, Anticancer and health protective properties of citrus fruit components. Asia Pac. J. Clin. Nutr. 11(1), 79–84 (2002)
M.J. Wargovich, Anticancer properties of fruits and vegetables. HortScience 35(4), 573–575 (2000)
T. Hirata, M. Fujii, K. Akita, N. Yanaka, K. Ogawa, M. Kuroyanagi, D. Hongo, Identification and physiological evaluation of the components from Citrus fruits as potential drugs for anti-corpulence and anticancer. Bioorg. Med. Chem. 17(1), 25–28 (2009)
J.W. Eckert, I.L. Eaks, Postharvest disorders and diseases of citrus fruits. Citrus Ind. 5, 179–260 (1989)
A.A. Kader, Fruit maturity, ripening, and quality relationships. In International Symposium Effect of Pre-& Postharvest factors in Fruit Storage 485 pp. 203–208 (1997)
K.K. Singh, B.S. Reddy, Post-harvest physico-mechanical properties of orange peel and fruit. Journal of food engineering 73(2), 112–120 (2006)
Internet Article, Inside an Orange, http://www.citrusbr.com/en/orangejuice/?ins=03, Retrived on 04 April 2016
Y. Froelicher, D. Dambier, J.B. Bassene, G. Costantino, S. Lotfy, C. Didout, P. Ollitrault, Characterization of microsatellite markers in mandarin orange (Citrus reticulata Blanco). Mol. Ecol. Resour. 8(1), 119–122 (2008)
K. Dorji, C. Yapwattanaphun, Assessment of the genetic variability amongst mandarin (Citrus reticulata Blanco) accessions in Bhutan using AFLP markers. BMC Genet. 16(1), 1 (2015)
X. Zhang, A.P. Breksa, D.O. Mishchuk, C.E. Fake, M.A. O’Mahony, C.M. Slupsky, Fertilisation and pesticides affect mandarin orange nutrient composition. Food Chem. 134(2), 1020–1024 (2012)
R.W. Hodgson, Horticultural varieties of citrus. Division of Agricultural Sciences (1967)
Internet Article, Citrus Pages, http://citruspages.free.fr/mandarins.html#classification. Retrived on 20 August 2015
Mandarinorange Internet Article, https://en.wikipedia.org/wiki/Mandarin_orange. Retrived on 20 August 2015
Internet Article, Market Watch: The wild and elusive Dancy”. David Karp, LA Times. http://www.latimes.com/food/la-fo-marketwatch-20110128-story.html. Retrived on 20 August 2015
Internet Article, International Citrus Genomics Consortium, http://www.citrusgenome.ucr.edu/. Retrived on 20 August 2015
N. Miyazawa, A. Fujita, K. Kubota, Aroma character impact compounds in Kinokuni mandarin orange (Citrus kinokuni) compared with Satsuma mandarin orange (Citrus unshiu). Biosci. Biotechnol. Biochem. 74(4), 835–842 (2010)
E. Iwata, H. Hotta, M. Goto, Hypolipidemic and bifidogenic potentials in the dietary fiber prepared from Mikan (Japanese mandarin orange: Citrus unshiu) albedo. J. Nutr. Sci. Vitaminol. 58(3), 175–180 (2012)
J. Wang, H. Hao, R. Liu, Q. Ma, J. Xu, F. Chen, X. Deng, Comparative analysis of surface wax in mature fruits between Satsuma mandarin (Citrus unshiu) and ‘Newhall’navel orange (Citrus sinensis) from the perspective of crystal morphology, chemical composition and key gene expression. Food Chem. 153, 177–185 (2014)
T.K. Bera, J. Nagaraju, Electrical impedance spectroscopic studies on broiler chicken tissue suitable for the development of practical phantoms in multifrequency EIT. J. Electr. Bioimpedance 2(1), 48–63 (2011)
K.S. Cole, Permeability and impermeability of cell membranes for ions. Quant. Biol. 8, 110–122 (1940)
T. Yamamoto, Y. Yamamoto, Analysis for the change of skin impedance. Med. Biol. Eng. Comput. 15(3), 219–227 (1977)
P. Zoltowski, On the electrical capacitance of interfaces exhibiting constant phase element behaviour. Electroanal. Chem. 443, 149–154 (1998)
M.I.N. Zhang, D.G. Stout, J.H.M. Willison, Plant tissue impedance and cold acclimation: a re-analysis. J. Exp. Bot. 43, 263–266 (1992)
L. Wu, Y. Ogawa, A. Tagawa, Electrical impedance spectroscopy analysis of eggplant pulp and effects of drying and freezing–thawing treatments on its impedance characteristics. J. Food Eng. 87, 274–280 (2008)
M. Itagaki, A. Taya, K. Watanabe, K. Noda, Deviations of capacitive and inductive loops in the electrochemical impedance of a dissolving iron electrode. Anal. Sci. 18, 641–644 (2002)
S. Skale, V. Dolecˇek, M. Slemnik, Substitution of the constant phase element by Warburg impedance for protective coatings. Corros. Sci. 49, 1045–1055 (2007)
S. Ricciardi, J.C. Ruiz-Morales, P. Nunez, Origin and quantitative analysis of the constant phase element of a platinum SOFC cathode using the state-space model. Solid State Ionics 180, 1083–1090 (2009)
Y. Ando, K. Mizutani, N. Wakatsuki, Electrical impedance analysis of potato tissues during drying. J.Food Eng. 121, 24–31 (2014)
B. Hirschorn, M.E. Orazem, B. Tribollet, V. Vivier, I. Frateur, M. Musianid, Constant-Phase-Element Behavior Caused by Resistivity Distributions in Films. J. Electrochem. Soc. 157 _12_ C452-C457 _2010_0013-4651/2010/157_12_/C452/6/$28.00 © The Electrochemical Society
B. Hirschorn, M.E. Orazem, B. Tribollet, V. Vivier, I. Frateur, M. Musiani, Constant-phase-element behavior caused by resistivity distributions in films I. Theory. J. Electrochem. Soc. 157(12), C452–C457 (2010)
Acknowledgements
All the authors acknowledge the NIT Agartala (NITA), Tripura, India for providing the research facilities to conduct and complete the research work. All authors also acknowledge the BMS College of Engineering, Bangalore, India and the Bidhan Chandra Krishi Viswavidyalaya (BCKV), Mohanpur, West Bengal, India.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
There is no conflict of interest for this research work presented in this manuscript.
Additional information
Practical applications (EIS studies conducted on the mandarin orange ripening analyze the orange ripening process in terms of its bioimpedance variations and construct the equivalent circuit. EIS studies conducted on the mandarin oranges establishes the relationship between the bioimpedance variation and ripening states which can be potentially utilized to noninvasively study the orange ripening. It is observed that the results obtained from the EIS studies also indicate that the orange impedance gradually increases and the weight gradually decreases with the ripening time. Conducting an electrical impedance spectroscopic studies on orange we can analyze the ripening stages and predict the suitable ripening state with desirable taste, quality and food content for mandarin oranges noninvasively. Thus EIS studies on the mandarin orange ripening not only help us to the optimum ripening state of orange but also it will help the researchers to analyze its physiological changes, taste, and nutrient levels).
Rights and permissions
About this article
Cite this article
Chowdhury, A., Singh, P., Bera, T.K. et al. Electrical impedance spectroscopic study of mandarin orange during ripening. Food Measure 11, 1654–1664 (2017). https://doi.org/10.1007/s11694-017-9545-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11694-017-9545-y