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Journal of Food Measurement and Characterization

, Volume 11, Issue 4, pp 1531–1541 | Cite as

Effect of different solvents on volatile and non-volatile constituents of red bell pepper (Capsicum annuum L.) and their in vitro antioxidant activity

  • Shruthi Prabakaran
  • Lavanya Ramu
  • Sudha Veerappan
  • Brindha Pemiah
  • Niraimathi KannappanEmail author
Original Paper

Abstract

The fresh fruit of Capsicum annuum L. (red bell pepper) was successively extracted using n-hexane, chloroform, ethyl acetate, ethanol and their percentage yield was calculated. The effectiveness of each extract on chemical composition and antioxidant activity was studied. The qualitative phytochemical evaluation of each extract of bell pepper was done by HPTLC and Gas chromatography–mass spectroscopy (GC-MS) analysis. The total content of phenols, flavonoids and carotenoids were estimated by standard chemical methods. Further, the antioxidant potential of each extract was measured via DPPH and reducing power assays. Gas chromatography–mass spectroscopy analysis showed that the majority of compounds were related to phenols and flavonoids. Further analysis of the extract by HPTLC verified the presence of different types of phenolic compounds in addition to flavonoids and carotenoids. Among the different solvent extracts analyzed, total phenolic content was higher in ethanol extract (7.136 ± 0.03%, w/w) whereas ethyl acetate extract showed the presence of higher flavonoid content (4.0521 ± 0.03%, w/w). The ethanol and ethyl acetate extracts of the fruit of C. annuum exhibited the highest radical scavenging activity with inhibition percentage of 53.66 and 49.55% at a concentration of 254 µg/ml. Based on the biochemical analysis and phytochemical screening, we conclude that C. annuum possess potent antioxidant potential and this ability of the extract is attributed to the presence of rich polyphenolic compounds.

Keywords

Antioxidant activity Bell pepper GC-MS analysis HPTLC 

Notes

Acknowledgements

The authors kindly acknowledge Honorable Vice chancellor of SASTRA University, Thanjavur for his financial support and facilities to carry out this research work.

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Shruthi Prabakaran
    • 1
  • Lavanya Ramu
    • 2
  • Sudha Veerappan
    • 2
  • Brindha Pemiah
    • 2
  • Niraimathi Kannappan
    • 2
    Email author
  1. 1.School of Chemical and BiotechnologySASTRA UniversityThanjavurIndia
  2. 2.Centre for Advanced Research in Indian System of Medicine (CARISM)SASTRA UniversityThanjavurIndia

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