Nutritional, functional, thermal and structural characteristics of Citrullus lanatus and Limonia acidissima seed flours

  • Sachin K. Sonawane
  • Mayuri B. Bagul
  • Jean Guy LeBlanc
  • Shalini S. Arya
Original Paper


Seeds from fruits such as Citrullus (C.) lanatus (watermelon) and Limonia (L.) acidissima (wood apple) are not commonly utilized but could be suitable in numerous food formulations. It was shown that the protein content of defatted seed flours was 71.38 and 49.51 % and that these contained considerable amounts of minerals such as Na, Mn, Mg, K, Cu, Fe and Zn. The defatted L. acidissima seed flour was superior to C. lanatus in essential amino acids. The flours obtained from both seeds were also evaluated for functional properties and characterized by X-ray diffraction, differential scanning calorimeter and scanning electron microscope (SEM). Amorphous nature was observed in defatted C. lanatus and L. acidissima flours due to the low percentage of degree of crystallinity. Spherical morphologies were observed through SEM. The exothermic peak was recorded in defatted C. lanatus and L. acidissima flour.


Citrullus lanatus Limonia acidissima Functional property SEM XRD 



This work was supported by University Grant Commission (UGC), Government of India. Authors would like to thank RB-CFBP Lab, SNDT women university, Santracruz, Mumbai and Department of Biochemistry and Cell biology, Sankara Nethralaya, Chennai for providing the facility of mineral and amino acid analysis.


  1. 1.
    M.S. Teotia, P. Ramakrishna, Chemistry and technology of melon seeds. J. Food Sci. Technol. 21, 332–340 (1984)Google Scholar
  2. 2.
    I.N.D. Godawa, M. Jalali, Studies on juice making from watermelon fruits. Indian Food Pack. 49(3), 33–41 (1995)Google Scholar
  3. 3.
    A.G. Waghmare, S.S. Arya, Use of fruit by-products in the preparation of hypoglycemic thepla: indian unleavened vegetable flat bread. J. Food Process. Preserv. 38(3), 1198–1206 (2014)CrossRefGoogle Scholar
  4. 4.
    J. Morton, Wood apple, in Fruits of Warm. (Florida Flare Books, 1987), pp 190–191Google Scholar
  5. 5.
    Y. Saima, A.K. Das, K.K. Sarkar, A.K. Sen, P. Sur, An antitumor pectic polysaccharide from Feronia limonia. Int. J. Biol. Macromol. 27(5), 333–335 (2000)CrossRefGoogle Scholar
  6. 6.
    M.M. Rahman, A.I. Gray, Antimicrobial constituents from the stem bark of Feronia limonia. Phytochemistry 59(1), 73–77 (2002)CrossRefGoogle Scholar
  7. 7.
    S.M. Ahamed, S.K. Swamy, K.N. Jayaverra, J.V. Rao, S. Kumar, Anti inflammatory, antipyretic and analgesic activity of methanolic extract of Feronia limonia. Pharmacology 3, 852–857 (2008)Google Scholar
  8. 8.
    D.K. Patel, R. Kumar, D. Laloo, S. Hemalatha, Diabetes mellitus: an overview on its pharmacological aspects and reported medicinal plants having ant diabetic activity. Asian Pac. J. Trop. Biomed. 2(5), 411–420 (2012)CrossRefGoogle Scholar
  9. 9.
    R. Vidhya, A. Narain, Formulation and evaluation of preserved products utilizing under exploited fruit, wood apple (Limonia acidissima). Am. Eurasian J. Agric. Environ. Sci. 10(1), 112–118 (2011)Google Scholar
  10. 10.
    S. Sonawane, S.S. Arya, Antioxidant activity of jambhul, wood apple, ambadi and ambat chukka: an indigenous lesser known fruits and vegetables of India. Adv. Food Sci. Technol. 5(03), 270–275 (2013)Google Scholar
  11. 11.
    S.K. Sonawane, S.S. Arya, Effect of drying and storage on bioactive components of jambhul and wood apple. J. Food Sci. Technol. 52(5), 2833–2841 (2015)CrossRefGoogle Scholar
  12. 12.
    AOAC, Official methods of analysis of Association of Official Analytical Chemists (AOAC) International 16th edn. (AOAC, Washington, DC, 1995)Google Scholar
  13. 13.
    A. Gratzfeld-Huesgen, Sensitive and reliable amino acid analysis in protein hydrolysates using the Agilent 1100 series HPLC. Technical Note. Agilent Technologies. Publication number 5968–5658 (1998). Available from:
  14. 14.
    M.I. Akpata, P.I. Akubor, Chemical composition and selected functional properties of sweet orange (Citrus sinensis) seed flour. Plant Food Hum. Nutr. 54, 353–362 (1999)CrossRefGoogle Scholar
  15. 15.
    O.J. Olayemi, A.R. Oyi, T.S. Allagh, Comparative evaluation of maize, rice and wheat starch powder as pharmaceutical excipients. Niger. J. Pharm. Sci. 7(1), 131–138 (2008)Google Scholar
  16. 16.
    H.W. Leach, L.D. McCowen, T.J. Schoch, Structure of the starch granules. I. Swelling and solubility patterns of various starches. Cereal Chem. 36, 534–544 (1959)Google Scholar
  17. 17.
    H.N. Liang, C.H. Tang, pH-dependent emulsifying properties of pea [Pisum sativum (L.)] proteins. Food Hydrocoll. 33, 309–319 (2013)CrossRefGoogle Scholar
  18. 18.
    A. Jyothi, P. Kaul, Nutritional potential, bioaccessibility of minerals and functionality of watermelon (Citrullus vulgaris) seeds. LWT 44(8), 1821–1826 (2011)CrossRefGoogle Scholar
  19. 19.
    B.L. Penuel, E.M. Khan, M.O. Maitera, Properties of proximate composition and elemental analysis of Citrullus vulgaris (Guna) seed. Bull. Environ. Pharmocol. Life Sci. 2(2), 39–46 (2013)Google Scholar
  20. 20.
    S.A. Joshi, Nutrition and Dietetics, 2nd edn. (Tata McGraw-Hill Publishing Company Ltd, New Delhi, 2007)Google Scholar
  21. 21.
    D.H. Feyssa, J.T. Njoka, Z. Asfaw, M.M. Nyangito, Wild edible fruits of importance for human nutrition in semi-arid parts of East Shewa Zone, Ethiopia: associated indigenous knowledge and implications to food security. Pak. J. Nutr. 10, 40–50 (2011)CrossRefGoogle Scholar
  22. 22.
    F.S. El-safy, R.H. Salem, M.E.A. El-ghany, Chemical and nutritional evaluation of different seed flours as novel sources of protein. World J. Dairy Food Sci. 7(1), 59–65 (2012)Google Scholar
  23. 23.
    C.S. Lin, J.F. Zayas, Functionality of defatted corn germ proteins in a model system: fat binding and water retention. J. Food Sci. 52, 1308–1311 (1987)CrossRefGoogle Scholar
  24. 24.
    S.K. Shate, D.K. Salunkhe, Functional properties of the Great Northern bean (Phaseolus vulgaris L.) proteins: emulsion, foaming, viscosity and gelation properties. J. Food Sci. 46, 71–74 (1981)CrossRefGoogle Scholar
  25. 25.
    M.S. Butt, R. Batool, Nutritional and functional properties of some promising legumes protein isolates. Pak. J Nutr. 9(4), 373–379 (2010)CrossRefGoogle Scholar
  26. 26.
    L. Kanig-Joseph, L. Leon, A.L. Herbert, The Theory and Practice of Industrial Pharmacy, 3rd edn. (Lea & Febiger, Philadelphia, 1986)Google Scholar
  27. 27.
    F.C.K. Ocloo, D. Bansa, R. Boatin, T. Adom, W.S. Agbemavor, P.O.B. Lg, Physico-chemical, functional and pasting characteristics of flour produced from Jackfruits (Artocarpus heterophyllus) seeds. Agric. Biol. J. N. Am. 1(5), 903–908 (2010)CrossRefGoogle Scholar
  28. 28.
    J.E. Rickard, J.M.V. Blanshard, M. Asaoka, Effects of cultivar and growth season on the gelatinization properties of cassava (Manihot esculenta) starch. J. Sci. Food Agric. 59, 53–58 (1992)CrossRefGoogle Scholar
  29. 29.
    J.E. Kinsella, S. Damodaran, B. German, Physicochemical and functional properties of oilseed protein with emphasis on protein, in New Protein Food Seed Storage Protein, vol. 5, ed. by A.M. Altschual, H.L. Wilcke (Academic Press, Orlando, 1985), p. 107CrossRefGoogle Scholar
  30. 30.
    K.A. Athmaselvi, C. Kumar, M. Balsubramanian, I. Roy, Thermal, structural, and physical properties of freeze dried tropical fruit powder. J. Food Process. 2014, 1–28 (2014)CrossRefGoogle Scholar
  31. 31.
    C. Zhang, X. Liu, H. Qiang, K. Li, J. Wang, D. Chen, Y. Zhuang, Inhibitory effects of rosa roxburghii tratt juice on in vitro oxidative modification of low density lipoprotein and on the macrophage growth and cellular cholesteryl ester accumulation induced by oxidized low density lipoprotein. Clin. Chim. Acta 313(1), 37–43 (2001)CrossRefGoogle Scholar
  32. 32.
    T. Theivasanthi, M. Alagar, An insight analysis of nano sized powder of jackfruit seed. Nano Biomed. Eng. 3(3), 163–168 (2011)CrossRefGoogle Scholar
  33. 33.
    U. Dharmaraj, P. Parameswara, R. Somashekar, N.G. Malleshi, Effect of processing on the microstructure of finger millet by X-ray diffraction and scanning electron microscopy. J. Food Sci. Technol. 51(3), 494–502 (2014)CrossRefGoogle Scholar
  34. 34.
    N. Sharon, H. Lis, Lectins with a sweet tooth: functions in cell recognition. Essays Biochem. 30, 59–75 (1995)Google Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Sachin K. Sonawane
    • 1
  • Mayuri B. Bagul
    • 1
  • Jean Guy LeBlanc
    • 2
  • Shalini S. Arya
    • 1
  1. 1.Food Engineering and Technology DepartmentInstitute of Chemical TechnologyMumbaiIndia
  2. 2.CERELA-CONICETSan Miguel de TucumanArgentina

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