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Feasibility of colloidal silver SERS for rapid bacterial screening

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Abstract

This study reports the feasibility of citrate-reduced colloidal silver surface-enhanced Raman scattering (SERS) for differentiating three important food borne pathogens, E. coli, Listeria, and Salmonella. FT-Raman and SERS spectra of both silver colloids and colloid-K3PO4 mixtures were collected and analyzed to evaluate the reproducibility and stability of silver colloids fabricated in a batch-production process. The results suggest that the reproducibility of the colloids over the batch process is high and that their binding effectiveness remains consistent over a 60-day storage period. Two specific SERS bands at 712 and 390 cm−1 were identified and used to develop simple 2-band ratios for differentiating E. coli-, Listeria-, and Salmonella-colloid mixtures with a 100% success. These results indicate that colloidal silver SERS technique may be a practical alternative method suitable for routine and rapid screening of E. coli, Listeria, and Salmonella bacteria.

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Authors and Affiliations

  1. Food Safety Laboratory, Henry A. Wallace Beltsville Agricultural Research Center, USDA, Building 303, BARC-East, 10300 Baltimore Avenue, Beltsville, MD, 20705, USA

    Yongliang Liu, Kuanglin Chao, Xiangwu Nou & Yud-Ren Chen

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  1. Yongliang Liu
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  2. Kuanglin Chao
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  3. Xiangwu Nou
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  4. Yud-Ren Chen
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Correspondence to Kuanglin Chao.

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Liu, Y., Chao, K., Nou, X. et al. Feasibility of colloidal silver SERS for rapid bacterial screening. Sens. & Instrumen. Food Qual. 3, 100–107 (2009). https://doi.org/10.1007/s11694-008-9064-y

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  • DOI: https://doi.org/10.1007/s11694-008-9064-y

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