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Analysis of the multicopper oxidase gene regulatory network of Aeromonas hydrophila

  • Research Article
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Systems and Synthetic Biology

Abstract

Multicopper oxidase (MCO) is an enzyme which involves in reducing the oxygen in a four electron reduction to water with concomitant one electron oxidation of reducing the substrate. We have generated the 3-D structure of MCO by homology modeling and validated on the basis of free energy while 90.4 % amino acid residues present in allowed regions of Ramachandran plot. The screening of potential hazardous aromatic compounds for MCO was performed using molecular docking. We obtained Sulfonaphthal, Thymolphthalein, Bromocresol green and Phloretin derivatives of phenol and aromatic hydrocarbon were efficient substrates for MCO. The phylogeny of MCO reveals that other bacteria restrain the homologous gene of MCO may play an important role in biodegradation of aromatic compounds. We have demonstrated the gene regulatory network of MCO with other cellular proteins which play a key role in gene regulation. These findings provide a new insight for oxidization of phenolic and aromatic compounds using biodegradation process for controlling environmental pollution.

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Acknowledgments

Authors are grateful to A.K. Singh, Satya Prakash and Pritee Singh for providing the suggestions, encouragement and fruitful discussion during preparation of this manuscript. Authors are also grateful to reviewers for their helpful suggestions.

Conflict of interest

There is no competing interest.

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Authors and Affiliations

  1. Institute of Systems and Synthetic Biology, Universite d’Evry Val d’Essonne-Genopole-CNRS UPS3201, Batiment Geneavenir 6, Genopole Campus 1, 5 rue Henri Desbruères, 91030, Evry Cedex, France

    Vijai Singh

  2. National Bureau of Fish Genetic Resources, Canal Ring Road, PO-Dilkusha, Lucknow, 226002, India

    Vijai Singh, Indra Mani & Dharmendra Kumar Chaudhary

  3. Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi, 221005, India

    Indra Mani

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  1. Vijai Singh
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  2. Indra Mani
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  3. Dharmendra Kumar Chaudhary
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Correspondence to Vijai Singh.

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Singh, V., Mani, I. & Chaudhary, D.K. Analysis of the multicopper oxidase gene regulatory network of Aeromonas hydrophila . Syst Synth Biol 6, 51–59 (2012). https://doi.org/10.1007/s11693-012-9096-9

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  • DOI: https://doi.org/10.1007/s11693-012-9096-9

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