Convergent Patterns of Body Shape Differentiation in Four Different Clades of Poeciliid Fishes Inhabiting Sulfide Springs
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We investigated replicated differentiation in four lineages of livebearing fishes (two with the genus Poecilia and two within Gambusia), which inhabit freshwater habitats and have also colonized sulfide springs in Oklahoma, Mexico, and Venezuela. Sulfide springs are characterized by extreme hypoxia and high concentrations of toxic hydrogen sulfide, which provide a strong source of divergent selection compared to adjacent non-sulfidic habitats. Using geometric morphometric analysis of body shape, we found that sulfide spring populations significantly differ from relatives from regular freshwater habitats in all four lineages investigated. Differentiation is characterized by strong patterns of convergent evolution, with sulfide spring populations consistently exhibiting an increase in head size, even though the magnitude and nature differences varied across lineages. Head size is strongly correlated with an increase in gill size in sulfide spring populations of the genus Poecilia, which facilitates efficient oxygen acquisition in the hypoxic H2S-environment and directly affects survival. The convergent patterns of differentiation support previous findings about the effects of sulfide on trait evolution.
KeywordsAdaptation Convergent evolution Divergent selection Gambusia Hydrogen sulfide Poecilia
We thank N. Franssen and C. Tobler for help in the field and D. Hendrickson for help with specimen loans. We are indebted to K. Winemiller for continuous support and for sharing information about sites in Venezuela. N. Franssen, M. Plath, R. Riesch, and C. Tobler kindly provided comments on an earlier version of the manuscript. The Mexican government (CONAPESCA: DGOPA/16986/191205/8101, DGOPA/02232/230706/1079, DGOPA.06192.240608-1562, DGOPA.00093.120110.-0018, and SGPA/DGVS/04751/08) and the United States National Park Service Chickasaw NRA (CHIC-2007-SCI-0001) kindly provided collection permits. Financial support came from the National Geographic Society.
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