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Genotyping of Toxoplasma gondii Strains from Goats in Jahrom District, Southern Iran

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Abstract

Purpose

Toxoplasma gondii is transmitted congenitally or acquired by consumption of food and water contaminated with cysts or oocysts. This study aimed at genotyping T. gondii strains from slaughtered goats in Jahrom.

Methods

A total of 561 specimens (heart, diaphragm, and tongue) from 187 slaughtered goats were collected from Jahrom slaughterhouse. After DNA extraction, the T. gondii strains were genotyped by the nested PCR–RFLP based on GRA6 and 3ʹ, and 5ʹ ends of the SAG2 gene.

Results

T. gondii infection was present in 18.2% of cases. Among the examined organs, the diaphragm was more disposed to the infection (10.2%). Furthermore, infection rates of the heart and tongue were 8.6% and 3.7%, respectively. Concurrent infection in the heart and diaphragm, tongue and diaphragm, and heart and tongue were 3.2%, 0.5%, and 0.5%, respectively. In genotyping experiments, genotype I was the most frequent genotype of T. gondii (58.8%), followed by type II (23.5%), type III (11.8%), and a combination of type I and II (5.9%).

Conclusions

The results of this study showed the presence of different genotypes of T. gondii in goats including three major and mixed genotypes. These results can be useful in toxoplasmosis control and prevention.

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Acknowledgements

The authors wish to thank Vice Canceller of Jahrom University of Medical Sciences for its financial support to accomplish this research project.

Funding

This study was supported by the Deputy of Jahrom University of Medical Sciences for its financial support to implement the project (IR.JUMS.REC.1396.097) awarded to the Hassan Rezanezhad.

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Authors and Affiliations

  1. Department of Parasitology, Jahrom University of Medical Sciences, Jahrom, Iran

    Riam Sadooni, Hassan Rezanezhad, Kavos Solhjoo & Belal Armand

  2. Zoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, Iran

    Hassan Rezanezhad & Kavos Solhjoo

  3. Research Center for Health Sciences, Institute of Health, Shiraz University of Medical Sciences, Shiraz, Iran

    Mohsen Kalantari

  4. Department of Health Education and Health Promotion, School of Health, Semnan University of Medical Sciences, Semnan, Iran

    Behrad Pourmohammadi

  5. Research Centre for Non-Communicable Diseases, Department of Advanced Medical Sciences and Technologies, School of Medicine, Jahrom University of Medical Sciences, Jahrom, Iran

    Saiedeh Erfanian

  6. Jahrom City Veterinary Office, Jahrom, Iran

    Masoud Esmi Jahromi

Authors
  1. Riam Sadooni
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  2. Hassan Rezanezhad
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  3. Kavos Solhjoo
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  4. Mohsen Kalantari
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  5. Behrad Pourmohammadi
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  6. Saiedeh Erfanian
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  7. Belal Armand
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  8. Masoud Esmi Jahromi
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Contributions

RS, HR, and MK conducted experiments, writing, and editing the figures of the first draft. HR, KS, and SE performed molecular biology and experimental setup to produce the particles. MK, BP, BA, and MEJ contributed to the design, analysis, and manuscript preparation. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Hassan Rezanezhad.

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Conflict of Interest

The authors declare that there is no conflict of interest regarding the publication of this article.

Ethics Approval

This study was approved by the Jahrom University of Medical Sciences Ethics Committee.

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Sadooni, R., Rezanezhad, H., Solhjoo, K. et al. Genotyping of Toxoplasma gondii Strains from Goats in Jahrom District, Southern Iran. Acta Parasit. 67, 454–459 (2022). https://doi.org/10.1007/s11686-021-00481-6

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  • DOI: https://doi.org/10.1007/s11686-021-00481-6

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