Skip to main content

Advertisement

Log in

Blastocystis Subtypes and Culture Characteristics of Isolates from Human Stools Related with the Presence of Gastrointestinal Symptoms: A Case–Control Study

  • Original Paper
  • Published:
Acta Parasitologica Aims and scope Submit manuscript

Abstract

Purpose

To stablish if Blastocystis subtypes influences gastrointestinal symptoms.

Methods

Case–control study. We obtained sequencing for Blastocysts subtyping from 13 patients with gastrointestinal symptoms (diarrhea or abdominal pain) and 12 from individuals without symptoms.

Results

12 sequences were from Subtype 2 and one from Subtype 3 in symptomatic individuals and nine samples were from Subtype 1, one from Subtype 2, and two from Subtype 3 in asymptomatic individuals. The prevalence of subtype 2 in symptomatic individuals was vastly different compared to the frequency in asymptomatic individuals (84.6% vs. 16.6%; OR 27.5 95% CI 3.2–233; Fisher exact test p = 0.0010201335). After in vitro culture, 22 isolates were obtained. Significant differences were observed for the 12 isolates from Subtype 2 that get a smaller number of total cells with dominant growth of vacuolar forms, compared with Subtypes 1 and 3, after eight days of culture.

Conclusion

Our results suggest that gastrointestinal symptoms in Colombian individuals with Blastocystis infection depend on the infecting subtype with peculiar phenotypic characteristics in in vitro culture.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Tan KSW (2008) New insights on classification, identification, and clinical relevance of Blastocystis spp. Clin Microbiol Rev 21:639–665. https://doi.org/10.1128/CMR.00022-08

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Ramírez JD, Sánchez LV, Bautista DC et al (2014) Blastocystis subtypes detected in humans and animals from Colombia. Infect Genet Evol 22:223–228. https://doi.org/10.1016/j.meegid.2013.07.020

    Article  PubMed  Google Scholar 

  3. Higuera A, Villamizar X, Herrera G et al (2020) Molecular detection and genotyping of intestinal protozoa from different biogeographical regions of Colombia. PeerJ 8:e8554. https://doi.org/10.7717/peerj.8554

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Jiménez PA, Jaimes JE, Ramírez JD (2019) A summary of Blastocystis subtypes in North and South America. Parasit Vectors 12:376. https://doi.org/10.1186/s13071-019-3641-2

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Ramírez JD, Flórez C, Olivera M et al (2017) Blastocystis subtyping and its association with intestinal parasites in children from different geographical regions of Colombia. PLoS ONE 12:e0172586. https://doi.org/10.1371/journal.pone.0172586

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. DANE. No Title. Censo Nac. Población y Vivienda (2018) 2019.https://www.dane.gov.co/index.php/estadisticas-por-tema/demografia-y-poblacion/censo-nacional-de-poblacion-y-vivenda-2018. Accessed 10 Aug 2019.

  7. Clark CG, Stensvold CR (2016) Blastocystis: isolation, xenic cultivation, and cryopreservation. Current protocols in microbiology. Wiley, Hoboken. https://doi.org/10.1002/cpmc.18

    Book  Google Scholar 

  8. Scanlan PD (2012) Blastocystis: past pitfalls and future perspectives. Trends Parasitol 28:327–334. https://doi.org/10.1016/j.pt.2012.05.001

    Article  PubMed  Google Scholar 

  9. Clark CG, van der Giezen M, Alfellani MA et al (2013) Recent developments in blastocystis research. Advances in parasitology. Academic Press, Cambridge, pp 1–32. https://doi.org/10.1016/B978-0-12-407706-5.00001-0

    Book  Google Scholar 

  10. Ajjampur SSR, Tan KSW (2016) Pathogenic mechanisms in Blastocystis spp.—Interpreting results from in vitro and in vivo studies. Parasitol Int 65:772–9. https://doi.org/10.1016/j.parint.2016.05.007

    Article  CAS  PubMed  Google Scholar 

  11. Stensvold CR, Clark CG (2016) Current status of Blastocystis: a personal view. Parasitol Int 65:763–771. https://doi.org/10.1016/j.parint.2016.05.015

    Article  PubMed  Google Scholar 

  12. de Melo GB, Roldan W, de Malta FM et al (2020) Culture isolation and molecular identification of Blastocystis sp. In Brazilian human isolates: Preliminary results. Rev Inst Med Trop Sao Paulo 62:1–5. https://doi.org/10.1590/S1678-9946202062051

  13. Zhang X, Qiao JY, Zhou XJ et al (2007) Morphology and reproductive mode of Blastocystis hominis in diarrhea and in vitro. Parasitol Res 101:43–51. https://doi.org/10.1007/s00436-006-0439-x

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This work was funded by Universidad del Quindío and COLCIENCIAS Grant Number: 111372553376.

Author information

Authors and Affiliations

Authors

Contributions

Deicy Muñoz-Sánchez: methodology, formal analysis, investigation, and data curation. Jessica Triviño-Valencia: methodology, formal analysis, and investigation. Fabiana Lora-Suarez: conceptualization, methodology, supervision, resources, writing—review and editing. Jorge Enrique Gómez-Marín: conceptualization, methodology, formal analysis, resources, data curation, writing—review and editing, and supervision.

Corresponding author

Correspondence to Jorge Enrique Gómez-Marín.

Ethics declarations

Conflict of interest

The authors declare having no conflict of interest related with the contents of this manuscript.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Muñoz-Sánchez, D., Triviño-Valencia, J., Lora-Suarez, F. et al. Blastocystis Subtypes and Culture Characteristics of Isolates from Human Stools Related with the Presence of Gastrointestinal Symptoms: A Case–Control Study. Acta Parasit. 66, 1466–1471 (2021). https://doi.org/10.1007/s11686-021-00419-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11686-021-00419-y

Keywords

Navigation