Abstract
Purpose
Endo-parasites of the bathypelagic king of herrings Regalecus glesne and oarfish Regalecus russelii are only known from few specimens opportunistically examined. As a consequence, there are few records of parasites from either Regalecus species. We report plerocercoid larvae of phyllobothriidean cestodes parasitizing an adult R. glesne stranded in Bahía de La Paz, Baja California Sur, Mexico.
Methods
Sixty-three plerocercoids were obtained from the intestine of R. glesne and characterized using morphological and molecular methods (nuclear 28S rDNA and mitochondrial cytochrome c oxidase I gene sequences).
Results
Following the morphological diagnostic criteria of scolex and muscle bands in the strobila, plerocercoids specimens were preliminary assigned to the genus Clistobothrium. Mitochondrial and nuclear DNA sequences indicate these plerocercoids correspond to Clistobothrium montaukensis Ruhnke, 1993.
Conclusion
Regalecus glesne is a new host known for C. montaukensis and this report is a new geographical record of C. montaukensis parasitizing species of the genus Regalecus previously known only from California and Florida, USA.
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Acknowledgements
We sincerely thank the staff of the Colección Ictiológica del Centro Interdisciplinario de Ciencias Marinas del Instituto Politécnico Nacional (CI-CICIMAR-IPN; http://coleccion.cicimar.ipn.mx/) for allowing us to obtain a sample of the parasites of their dissected R. glesne specimen. We are particularly thankful to José de la Cruz-Agüero for his permission to analyze parasites of this Regalecus specimen. We thank the financial support for the molecular analysis of parasites used in this study provided by the project Zooplankton and their parasites in Los Cabos region (iBOLD Code “ZPCR”). We deeply thank Patricia Cortés-Calva and Griselda Gallegos-Simental (Nodo del Código de Barras de la Vida, MEXBOL at CIBNOR, La Paz, Baja California Sur, Mexico, CONACYT 2017-280896 and 2018-295569) for helping us to extract, amplify and sequence cox1 gene. We also thank Ofelia Delgado, Andrea Jiménez-Marín, Laura Márquez, and Nelly López-Ortíz (Laboratorio Nacional de la Biodiversidad-IB-UNAM) for their valuable assistance with the generation of the nuclear 28S rDNA gene sequences. We also deeply thank Ariel Cruz-Villacorta for his valuable assistance with SEM observations (CIBNOR, La Paz, Baja California Sur, Mexico). We thank to two anonymous referees that greatly improved the present manuscript. This study was partially funded by the projects CONACYT CB-2012-178615-01, CB-2016-01-284201 granted to JG-G; and CONACYT 220408 and PAPIIT IN213520 granted to AO-F. HL-C was supported by a PhD CONACYT (A14618), PIFI-IPN (SIP20110012), and BEIFI-IPN (SIP20140497, 20150113, 20160495, 20171275) grants. All the authors are SNI fellows, and JG-G is also a COFAA-IPN and an EDI-IPN fellow.
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Lozano-Cobo, H., Gómez del Prado-Rosas, M.d.C., Silva-Segundo, C.A. et al. Molecular Identification of Plerocercoids of Clistobothrium montaukensis (Cestoda: Phyllobothriidea) Parasitizing the King of Herrings Regalecus glesne. Acta Parasit. 66, 1586–1592 (2021). https://doi.org/10.1007/s11686-021-00400-9
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DOI: https://doi.org/10.1007/s11686-021-00400-9