Activity In Vitro of 2-Chloro-N-[4-(4-Chlorophenyl)-2-Thiazolyl]Acetamide Against Promastigotes of Leishmania mexicana: An Apoptosis Inducer



Leishmaniasis is an infectious disease transmitted by insects that proliferate mainly in impoverished environments of tropical climates. In the absence of an effective vaccine, pharmacological treatment is the main tool to combat this disease. The objective of this work was to analyze the anti-leishmanial activity of 2-chloro-N-[4-(4-chlorophenyl)-2-thiazolyl] acetamide (AT) in promastigotes of Leishmania mexicana.


The biological activity of the compound was evaluated using a sulphorhodamine B cytotoxicity test and the integrity of the erythrocytes was evaluated by a lysis test. The anti-trypanosomatid activity was evaluated in vitro, a cell death assay was performed by flow cytometry (IP/Annexin V stain) and a parasite growth recovery assay was performed.


The AT showed a CC50 value of 0.031 µM for HeLa cells after 24 h of exposure, which did not induce erythrocyte lysis. On the other hand, the AT showed an IC50 value of 0.086 µM for L. mexicana (promastigote form) after 24 h of interaction. The compound was capable of inducing apoptosis in the parasites and did not allow recovery after 24 h of exposure.


This study provides valuable information with the objective of developing new drugs for the treatment of this disease, although more research on this molecule is needed to improve its biological activity.

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Data Availability

Data generated and analyzed during this study were included in this published article.


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The authors thanks to Estefanía Ramos-López, Roberto Meza-Romero and Eric Dumonteil for her appreciation of the manuscript. Mario Daniel Caba-Flores was a recipient of a Ph.D. fellowship from CONACyT, Mexico (451898).


This research was funded by the Universidad Veracruzana (Prize for interdisciplinary research 2019–2020).

Author information




Conceptualization: AR-L, DHR; Data curation: AR-L; Formal analysis: MDC-F, VAR-C, JL-D; Funding acquisition: DHR; Investigation: VAR-C, JL-D, DCV-O, ÁT, AYLF, ES-P, MDC-F; Project administration: AR-L, DHR; Resources: DHR, ES-P; Supervision: DHR, AL-M, AR-L; Validation: MDC-F, DHR, AL-M, ES-P, DCV-O, JL-D, VAR-C, AYLF, ÁT, AR-L; Visualization: DHR, AL-M, AR-L; Writing—original draft: MDC-F, DHR; Writing—review and editing: AL-M, AR-L.

Corresponding author

Correspondence to Angel Ramos-Ligonio.

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The project was reviewed and approved by the Ethical Committee of the Faculty of Chemical Sciences (FCQ/057/08/2019).

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Caba-Flores, M.D., Hernández-Romero, D., López-Monteon, A. et al. Activity In Vitro of 2-Chloro-N-[4-(4-Chlorophenyl)-2-Thiazolyl]Acetamide Against Promastigotes of Leishmania mexicana: An Apoptosis Inducer. Acta Parasit. (2021).

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  • Neglected tropical diseases
  • Aminothiazole derivatives
  • Leishmaniasis
  • Apoptosis
  • Infectious disease