Identification of Tapeworm Species in Genetically Characterised Grey Wolves Recolonising Central Europe

Abstract

Purpose

Restored role of the grey wolf in ecological networks of newly recolonized areas can be studied via surveys of parasite communities of this predator. As helminths circulating in multi-host systems, the tapeworms directly reflect wolves’ diet, while some species are also important from the One Health perspective. The Czech experienced centuries of wolves’ absence, however, now it is situated on the crossroad of recolonising wolves’ populations, which is opening questions of their role in ecological networks in this area and thus in sylvatic cycles of heteroxenous parasites.

Methods

Five wolf carcasses from this area were obtained and genetic affinity to a particular population was inspected. Tapeworms isolated from wolves’ intestines during necropsies were molecularly identified based on sequences of COI marker.

Results

Three wolf haplotypes (w1, w2, w14) correspond with the dominance of haplogroup 1 (w1, w2) within Central European lowland population and haplogroup 2 (w14) within the Carpathian population. Two Taenia spp. were revealed: T. krabbei in Central European population wolves and T. hydatigena in an individual from Carpathian population.

Conclusions

The results serve as a base for future monitoring and studies of the recolonising wolf population and its impact on ecosystems in the studied area to contribute to the hypothesis about differentiation of parasite communities in particular wolf population and higher parasite diversity and richness in established populations in comparison to newly settled ones.

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Availability of Data and Materials

All published sequential data are available in GenBank database under defined accession numbers.

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Acknowledgements

We are grateful to the Nature Conservation Agency of the Czech Republic for providing access to the wolves’ cadavers. The study was supported by the grant project CIGA 20185006 of Czech University of Life Sciences Prague, by the project IGA 104/2020/FVL provided by University of Veterinary and Pharmaceutical Sciences Brno and by the project Nr. CZ.02.1.01/0.0/0.0/16_019/0000787 “Fighting Infectious Diseases“ provided by the Ministry of Education Youth and Sports of the Czech Republic.

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J J, P H and D M designed the study. L F, J J and O D did the necropsies and collected samples. P H and B Č B did genetic analyses of wolves, K H and J J did molecular analyses of tapeworms. J J wrote the manuscript and all the authors read and approved the final manuscript.

Corresponding author

Correspondence to Jana Juránková.

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We have no conflicts of interests or competing interests.

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Juránková, J., Hulva, P., Bolfíková, B.Č. et al. Identification of Tapeworm Species in Genetically Characterised Grey Wolves Recolonising Central Europe. Acta Parasit. (2021). https://doi.org/10.1007/s11686-020-00327-7

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Keywords

  • Grey wolf
  • Recolonising
  • Haplotypes
  • Tapeworms